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Maximum Shortening Velocity and Myosin Heavy-chain Isoform Expression in Human Masseter Muscle Fibers

The Department of Orthodontics & Dentofacial Orthopedics, University of Pittsburgh, 3501 Terrace Street, Pittsburgh, PA 15261-1932, jjs6{at}pitt.edu

C.A. Branden

The Department of Orthodontics & Dentofacial Orthopedics, University of Pittsburgh, 3501 Terrace Street, Pittsburgh, PA 15261-1932

M.J. Horton

The Department of Orthodontics & Dentofacial Orthopedics, University of Pittsburgh, 3501 Terrace Street, Pittsburgh, PA 15261-1932

D.S. Carlson

Baylor College of Dentistry, Texas A&M University System Health Science Center

J.J. Sciote

The Department of Orthodontics & Dentofacial Orthopedics, University of Pittsburgh, 3501 Terrace Street, Pittsburgh, PA 15261-1932

While human masseter muscle is known to have unusual co-expression of myosin heavy-chain proteins, cellular kinetics of individual fibers has not yet been tested. Here we examine if myosin heavy-chain protein content is closely correlated to fiber-shortening speed, as previously reported in other human muscles, or if these proteins do not correlate well to shortening speeds, as has been demonstrated previously in rat muscle. Slack-test recordings of single, skinned human masseter fibers at 15°C revealed maximum shortening velocities generally slower and much more variable than those recorded in human limb muscle. The slowest fiber recorded had a maximum shortening velocity (V₀) value of 0.027 muscle lengths ·s^-1, several times slower than the slowest type I fibers previously measured in humans. By contrast, human limb muscle controls produced V₀ measurements comparable with previously published results. Analysis by gel electrophoresis found 63% of masseter fibers to contain pure type I MyHC and the remainder to co-express mostly type I in various combinations with IIA and IIX isoforms. Vo in masseter fibers forms a continuum in which no clear relationship to MyHC isoform content is apparent.

Key Words: V0 • slack test • skeletal muscle.

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Journal of Dental Research, Vol. 80, No. 9, 1845-1848 (2001)
DOI: 10.1177/00220345010800091401


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Morris, T.J. Articles by Sciote, J.J. Search for Related Content PubMed PubMed Citation Articles by Morris, T.J. Articles by Sciote, J.J. Social Bookmarking                         What's this?