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Effects of Dental Resins on TNF- -induced ICAM-1 Expression in Endothelial Cells
E.L. Kostoryz
University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA
P.Y. Tong
University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA
A.F. Strautman
William Jewell College, Liberty, MO, USA
A.G. Glaros
University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA
J.D. Eick
University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA
D.M. Yourtee
University of Missouri-Kansas City, Schools of Pharmacy and Dentistry, 2411 Holmes Street, M4-209, Kansas City, MO 64108, USA
Many reports have demonstrated inflammation after the placement of dental restorations. To explain this side-effect, we studied a biomarker in the inflammatory response. The intercellular adhesion molecule-1 (ICAM-1) is a key mediator for recruitment of leukocytes to the site of inflammation. Therefore, we investigated whether methacrylates (a BISGMA-based dental resin, BISGMA, and MAA) and CyracureTM UVR 6105, an epoxy monomer, could alter ICAM-1 expression in unstimulated and TNF-a-stimulated endothelial cells. Six-well plates with monolayers of human umbilical vein cells, ECV 304 (ATCC CRL 1998), were exposed to TNF-a (1 ng/mL) in the presence and absence of subtoxic and TC50 doses of chemicals for 24 hrs at 37°C/5% CO2. Several doses of TNF-a (0.5-2 ng/mL) were co-incubated with 100 µL of undiluted aqueous dental resin extracts. Cells were harvested and stained with mAB FITC-conjugated anti-human ICAM-1 (CD54). ICAM-1 expression was measured by flow cytometry. Cells expressed basal levels of ICAM-1, which was up-regulated by TNF-a but was not changed by all samples studied. Except for UVR 6105, the methacrylates significantly decreased ICAM-1 expression in TNF- -stimulated cells. These findings suggest that methacrylates may decrease the recruitment of leukocytes to sites of inflammation.
Key Words: endothelial cells • ICAM-1 expression • methacrylates • dental materials • TNF-a
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Journal of Dental Research, Vol. 80, No. 9,
1789-1792 (2001)
DOI: 10.1177/00220345010800090301
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