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Growth-hormone-stimulated Dentinogenesis in Lewis Dwarf Rat Molars

Department of Oral Biology, School of Dentistry, The University of Queensland, Brisbane, QLD 4072, Australia

H. Li

Department of Oral Biology, School of Dentistry, The University of Queensland, Brisbane, QLD 4072, Australia

Y. Xiao

Department of Oral Biology, School of Dentistry, The University of Queensland, Brisbane, QLD 4072, Australia

M.J. Waters

Department of Physiology & Pharmacology, The University of Queensland, Brisbane, QLD 4072, Australia

P.M. Bartold

Department of Oral Biology, School of Dentistry, The University of Queensland, Brisbane, QLD 4072, Australia, p.bartold{at}mailbox.uq.edu.au

In dentinogenesis, certain growth factors, matrix proteoglycans, and proteins are directly or indirectly dependent on growth hormone. The hypothesis that growth hormone up-regulates the expression of enzymes, sialoproteins, and other extracellular matrix proteins implicated in the formation and mineralization of tooth and bone matrices was tested by the treatment of Lewis dwarf rats with growth hormone over 5 days. The molar teeth were processed for immunohistochemical demonstration of bone-alkaline phosphatase, bone morphogenetic proteins-2 and -4, osteocalcin, osteopontin, bone sialoprotein, and Ell protein. Odontoblasts responded to growth hormone by more cells expressing bone morphogenetic protein, alkaline phosphatase, osteocalcin, and osteopontin. No changes were found in bone sialoprotein or E11 protein expression. Thus, growth hormone may stimulate odontoblasts to express several growth factors and matrix proteins associated with dentin matrix biosynthesis in mature rat molars.

Key Words: growth hormone • dentin matrix • teeth.

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Journal of Dental Research, Vol. 80, No. 8, 1742-1747 (2001)
DOI: 10.1177/00220345010800081201


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Young, W.G. Articles by Bartold, P.M. Search for Related Content PubMed PubMed Citation Articles by Young, W.G. Articles by Bartold, P.M. Social Bookmarking                         What's this?