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Autoantibodies to Human  6 Integrin in Patients with Oral Pemphigoid
K.C. Bhol
Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115
L. Goss
Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115
S. Kumari
Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115
J.E. Colon
Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115
A.R. Ahmed
Department of Oral Medicine and Diagnostic Sciences, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, razzaque_ahmed{at}hms.harvard.edu
Mucous membrane pemphigoid or cicatricial pemphigoid is a mucocutaneous blistering disease characterized by autoantibodies to different molecules in the basement membrane zone. Our objectives were to identify the target antigen recognized by sera from 20 untreated patients with pemphigoid disease limited to the oral cavity, and to determine the pathogenicity of autoantibodies in oral pemphigoid, with an organ culture model. We conducted indirect immunofluorescence, immunoblot, and immunoprecipitation assays, with accompanying absorption experiments, using normal human skin, conjunctiva and gingiva, bovine gingiva and a tumor cell line, which were reacted with sera from patients with oral pemphigoid, anti- 6 antibody, and control sera. Sera of oral pemphigoid patients selectively and specifically bound to human a6 integrin, a 120-kDa protein present in gingiva and the tumor cell line. Oral pemphigoid sera and anti-a6 antibody produced separation of epithelium from basement membrane (blister formation) of normal human buccal mucosa, after 48 hours, in organ culture.
Key Words: oral pemphigoid • human 6 integrin • cicatricial pemphigoid • anti-BMZ antibodies • organ culture model.
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Journal of Dental Research, Vol. 80, No. 8,
1711-1715 (2001)
DOI: 10.1177/00220345010800080601
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K. A. Rashid, J. N. H. Stern, and A. R. Ahmed
Identification of an Epitope within Human Integrin {alpha}6 Subunit for the Binding of Autoantibody and Its Role in Basement Membrane Separation in Oral Pemphigoid
J. Immunol.,
February 1, 2006;
176(3):
1968 - 1977.
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