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Cell-surface Proteoglycan Expression by Lymphocytes from Peripheral Blood and Gingiva in Health and Periodontal Disease
J.F. Manakil
School of Dentistry, The University of Queensland, Brisbane QLD 4000, Australia
P.B. Sugerman
School of Dentistry, The University of Queensland, Brisbane QLD 4000, Australia
H. Li
School of Dentistry, The University of Queensland, Brisbane QLD 4000, Australia
G.J. Seymour
School of Dentistry, The University of Queensland, Brisbane QLD 4000, Australia
P.M. Bartold
School of Dentistry, The University of Queensland, Brisbane QLD 4000, Australia, p.bartold{at}mailbox.uq.edu.au
Cell-surface proteoglycans are involved in lymphocyte migration and activation. This study investigated the expression of syndecan-1, syndecan-4, and glypican in peripheral blood lymphocytes and by lymphocytes in variously inflamed periodontal tissues. Gingival specimens from healthy, gingivitis, or chronic periodontitis sites were stained by means of antibodies against B- and T-lymphocytes and also syndecan-1, syndecan-4, and glypican. Syndecan-1 expression by peripheral blood mononuclear cells (PBMC) from healthy, gingivitis, and chronic periodontitis subjects was assessed by flow cytometry. Syndecan-1 was expressed by B-cells/plasma cells but not T-cells in both gingivitis and chronic periodontitis lesions. Both B-cells/plasma cells and T-cells in gingivitis and chronic periodontitis expressed syndecan-4. Glypican was expressed only by macrophages. Stimulation of PBMC with mitogens and growth factors modulated syndecan-1 expression in both the T- and B-cells. Thus, cell-surface proteoglycan expression by lymphocytes in periodontal inflammation is cell-type-specific and may be modulated by inflammation.
Key Words: cell-surface proteoglycans • lymphocytes • periodontal disease
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Journal of Dental Research, Vol. 80, No. 8,
1704-1710 (2001)
DOI: 10.1177/00220345010800080501
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