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Expression of Bone Matrix Protein mRNAs by Primary and Cloned Cultures of the Regenerative Phenotype of Human Periodontal Fibroblasts

Department of Dentistry, University of Queensland, Turbot Street, Brisbane, Queensland, 4000, Australia, s302219{at}student.uq.edu.au

H.R. Haase

Department of Dentistry, University of Queensland, Turbot Street, Brisbane, Queensland, 4000, Australia

P.M. Bartold

Department of Dentistry, University of Queensland, Turbot Street, Brisbane, Queensland, 4000, Australia

The successful regeneration of periodontal tissues is dependent, in part, on the ability of cells to reconstitute the mineralized tissues of cementum and bone. The aim of the present study was to characterize regeneration-associated cells in terms of their ability to express mineralized tissue macromolecules. Following guided tissue regeneration, cell cultures were established from regenerating tissue, periodontal ligament, and gingiva. Additionally, these cells were transfected, and single-cell-derived clones were established. Following treatment with platelet-derived growth factor-BB and insulin-derived growth factor-1, the presence of mRNA for alkaline phosphatase, osteocalcin, bone sialoprotein, osteopontin, and bone morphogenetic proteins-2 and -4 was assessed. The three cell types expressed similar mRNA levels for alkaline phosphatase, bone morphogenetic protein-2, and bone morphogenetic protein-4, whereas the expression of osteopontin, osteocalcin, and bone sialoprotein was greater in the periodontal ligament and regenerating tissue fibroblasts compared with the gingival fibroblasts. The two growth factors did not affect the expression of any of the genes. This study has identified markers that correlate with the known ability of periodontal ligament and regenerating tissue-derived fibroblasts to facilitate regeneration of the mineralized tissues of the periodontium.

Key Words: osteopontin • osteocalcin • bone sialoprotein • periodontal regeneration • fibroblast heterogeneity.

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Journal of Dental Research, Vol. 80, No. 7, 1665-1671 (2001)
DOI: 10.1177/00220345010800071301


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ivanovski, S. Articles by Bartold, P.M. Search for Related Content PubMed PubMed Citation Articles by Ivanovski, S. Articles by Bartold, P.M. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?