Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Fraser, H.S.
Right arrow Articles by Scott, D.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fraser, H.S.
Right arrow Articles by Scott, D.A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Elevated Systemic Concentrations of Soluble ICAM-1 (sICAM) are Not Reflected in the Gingival Crevicular Fluid of Smokers with Periodontitis

H.S. Fraser

Departments of Periodontology and Preventive Dentistry

R.M. Palmer

Departments of Periodontology and Preventive Dentistry, richard.m.palmer{at}kcl.ac.uk

R.F. Wilson

Dental Clinical Research, Guy's, King's, and St. Thomas' Dental Institute, Schools of Medicine and Dentistry, Floor 21, Guy's Hospital Tower, King's College London, London, SE1 9RT, UK

P.Y. Coward

Dental Clinical Research, Guy's, King's, and St. Thomas' Dental Institute, Schools of Medicine and Dentistry, Floor 21, Guy's Hospital Tower, King's College London, London, SE1 9RT, UK

D.A. Scott

Dental Clinical Research, Guy's, King's, and St. Thomas' Dental Institute, Schools of Medicine and Dentistry, Floor 21, Guy's Hospital Tower, King's College London, London, SE1 9RT, UK

Raised serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in smokers could have immunomodulatory effects in periodontitis. The aim of this study was to compare serum and gingival crevicular fluid (GCF) concentrations of sICAM-1 in smokers and non-smokers with periodontal disease. sICAM-1 in serum and GCF collected from age- and gender-matched smokers (n = 14) and non-smokers (n = 14) with periodontitis were measured by ELISA. Mean serum sICAM-1 concentrations were significantly elevated in smokers (331 ng mL-1), compared with non-smokers (238 ng mL-1, p = 0.008). However, the concentration of sICAM-1 in the GCF was significantly lower in the smokers (83 ng mL -1), compared with non-smokers (212 ng mL-1, p = 0.013). The difference between concentrations of sICAM-1 in GCF and serum was significant only in smokers (p < 0.001). Since GCF is a serum-derived tissue exudate, these results suggest that, in smokers, circulating sICAM-1 molecules are affected either in their passage from the periodontal microvasculature or within the periodontal tissues.

Key Words: cotinine • human • periodontitis • soluble ICAM-1 • smoking.

REFERENCES

  • Alavi AL, Palmer RM, Odell EW, Coward PY, Wilson RF (1995). Elastase in gingival crevicular fluid from smokers and non-smokers with chronic inflammatory periodontal disease. Oral Dis 1:110-114.[Medline] [Order article via Infotrieve]
  • Barnett CC, Moore EE, Mierau GW, Partrick DA, Biffl WL, Elzi DJ, et al. (1998). ICAM-1-CD18 interaction mediates neutrophil cytotoxicity through protease release. Am J Physiol 274:C1634-1644.[Medline] [Order article via Infotrieve]
  • Blann AD, Seigneur M., Steiner M., Miller JP, McCollum CN (1998). Circulating ICAM-1 and VCAM-1 in peripheral artery disease and hypercholesterolaemia: relationship to the location of atherosclerotic disease, smoking, and in the prediction of adverse events. Thromb Haemost 79:1080-1085.[Medline] [Order article via Infotrieve]
  • Duperray A., Languino L., Plescia J., McDowall A., Hogg N., Craig AG, et al. (1997). Molecular identification of a novel fibrinogen binding site on the first domain of ICAM-1 regulating leukocyte-endothelium bridging. J Biol Chem 272:435-441.[Abstract/Free Full Text]
  • Dustin ML, Rothlein R., Bhan AK, Dinarello CA, Springer TA (1986). Induction by IL-1 and interferon-gamma: tissue distribution, biochemistry, and function of a natural adherence molecule (ICAM-1). J Immunol 137:245-254.[Abstract]
  • Feyerabend C., Russell MA (1990). A rapid gas-liquid chromatographic method for the determination of cotinine and nicotine in biological fluids. J Pharm Pharmacol 42:450-452.[Medline] [Order article via Infotrieve]
  • Heatherton TF, Kozlowski LT, Frecker RC, Fagerstrom KO (1991). The Fagerstrom Test for Nicotine Dependence: a revision of the Fagerstrom Tolerance Questionnaire. Br J Addict 86:1119-1127.[CrossRef][Medline] [Order article via Infotrieve]
  • Jarvis MJ, Tunstall-Pedoe H., Feyerabend C., Vesey C., Saloojee Y. (1987). Comparison of tests used to distinguish smokers from nonsmokers. Am J Public Health 77:1435-1438.[Abstract/Free Full Text]
  • Kalra VK, Ying Y., Deemer K., Natarajan R., Nadler JL, Coates TD (1994). Mechanism of cigarette smoke condensate induced adhesion of human monocytes to cultured endothelial cells. J Cell Physiol 160:154-162.[CrossRef][Medline] [Order article via Infotrieve]
  • Koundouros E., Odell E., Coward PY, Wilson RF, Palmer RM (1996). Soluble adhesion molecules in serum of smokers and non-smokers, with and without periodontitis. J Periodont Res 31:596-599.[CrossRef][Medline] [Order article via Infotrieve]
  • Lehr HA, Frei B., Arfors KE (1994). Vitamin C prevents cigarette smoke-induced leukocyte aggregation and adhesion to endothelium in vivo. Proc Natl Acad Sci USA 91:7688-7692.[Abstract/Free Full Text]
  • MacNee W., Wiggs B., Belzberg AS, Hogg JC ( 1989). The effect of cigarette smoking on neutrophil kinetics in the lungs. N Engl J Med 321:924-928.[Abstract]
  • McGuire JR, McQuade MJ, Rossman JA, Garnick JJ, Sutherland DE, Scheidt MJ, et al. (1989). Cotinine in saliva and gingival crevicular fluid of smokers with periodontal disease. J Periodontol 60:176-181.[Medline] [Order article via Infotrieve]
  • Meekin TN, Wilson RF, Scott DA, Ide M., Palmer RM (2000). Laser Doppler flowmeter measurement of relative gingival and forehead skin blood flow in light and regular smokers during and after smoking. J Clin Periodontol 27:236-242.[Medline] [Order article via Infotrieve]
  • Miller J., Knorr R., Ferrone M., Houdei R., Carron CP, Dustin ML (1995). Intercellular adhesion molecule-1 dimerization and its consequences for adhesion mediated by lymphocyte function associated antigen-1. J Exp Med 182:1231-1241.[Abstract/Free Full Text]
  • Palmer RM, Scott DA, Meekin TN, Wilson RF, Poston RN, Odell EW (1999). Potential mechanisms of susceptibility to periodontitis in tobacco smokers. J Periodont Res 34:363-369.[CrossRef][Medline] [Order article via Infotrieve]
  • Pitzer JE, Del Zoppo GJ, Schmid-Schonbein GW (1996). Neutrophil activation in smokers. Biorheology 33:45-58.[CrossRef][Medline] [Order article via Infotrieve]
  • Reilly PL, Woska JR Jr, Jeanfavre DD, McNally E., Rothlein R., Bormann BJ (1995). The native structure of intercellular adhesion molecule-1 (ICAM-1) is a dimer. Correlation with binding to LFA-1. J Immunol 155:529-532.[Abstract]
  • Rezavandi K., Palmer RM, Odell EW, Scott DA, Wilson RF Expression of ICAM-1 and E-Selectin in gingival tissues of smokers and non-smokers. J Oral Pathol (in press).
  • Rothlein R., Mainolfi EA, Czajkowski M., Marlin SD (1991). A form of circulating ICAM-1 in human serum. J Immunol 147:3788-3793.[Abstract]
  • Russell Mah, Feyerabend C., Cole PV ( 1976). Plasma nicotine levels after cigarette smoking and chewing nicotine gum. BMJ 1(6017):1043-1046.[Abstract/Free Full Text]
  • Russell MA, Jarvis M., Iyer R., Feyerabend C. (1980). Relation of nicotine yield of cigarettes to blood nicotine concentrations in smokers. BMJ 280:972-976.[Abstract/Free Full Text]
  • Ryder MI, Fujitaki R., Lebus S., Mahboub M., Faia B., Muhaimin D., et al. (1998). Alterations of neutrophil L-selectin and CD 18 expression by tobacco smoke: implications for periodontal diseases. J Periodont Res 33:359-368.[CrossRef][Medline] [Order article via Infotrieve]
  • Schwartz J., Weiss ST (1994). Cigarette smoking and peripheral blood leukocyte differentials. Ann Epidemiol 4:236-242.[Medline] [Order article via Infotrieve]
  • Scott DA, Todd DH, Wilson RF, Coward PY, Odell EW, Poston RN, et al. (2000a). The acute influence of tobacco smoking on adhesion molecule expression on monocytes and neutrophils and on circulating adhesion molecule levels in vivo. Addiction Biol 5:195-205.
  • Scott DA, Stapleton JA, Wilson RF, Sutherland G., Palmer RM, Coward PY, et al. (2000b). Dramatic decline in circulating intercellular adhesion molecule-1 concentration on quitting tobacco smoking. Blood Cells Mol Dis 26:255-258.[CrossRef][Medline] [Order article via Infotrieve]
  • Van de Stolpe A., Van der Saag PT (1996). Intercellular adhesion molecule-1. J Mol Med 74:13-33.[Medline] [Order article via Infotrieve]
  • Woodward M., Lowe GD, Rumley A., Tunstall-Pedoe H. (1998). Fibrinogen as a risk factor for coronary heart disease and mortality in middle-aged men and women. The Scottish Heart Health Study. Eur Heart J 19:55-62.[Abstract/Free Full Text]
  • Woska JR Jr, Morelock MM, Jeanfavre DD, Caviness GO, Bormann BJ, Rothlein R. (1998). Molecular comparison of soluble intercellular adhesion molecule (sICAM)-l and sICAM-3 binding to lymphocyte function-associated antigen-1. J Biol Chem 273:4725-4733.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 80, No. 7, 1643-1647 (2001)
DOI: 10.1177/00220345010800070901
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Fraser, H.S.
Right arrow Articles by Scott, D.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fraser, H.S.
Right arrow Articles by Scott, D.A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?