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Antifungal Resistance of Candidal Biofilms Formed on Denture Acrylic in vitro
J. Chandra
Center for Medical Mycology, University Hospitals of Cleveland and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-5028
P.K. Mukherjee
Center for Medical Mycology, University Hospitals of Cleveland and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-5028
S.D. Leidich
Center for Medical Mycology, University Hospitals of Cleveland and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-5028
F.F. Faddoul
Department of Restorative Dentistry, School of Dentistry, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106-4905
L.L. Hoyer
Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign, 2522 VMBSB, 2001 S. Lincoln Ave., Urbana, IL 61802
L.J. Douglas
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom
M.A. Ghannoum
Center for Medical Mycology, University Hospitals of Cleveland and Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106-5028, mag3{at}po.cwru.edu
Denture biofilms represent a protective reservoir for oral microbes. The study of the biology of Candida in these biofilms requires a reliable model. A reproducible model of C. albicans denture biofilm was developed and used to determine the susceptibility of two clinically relevant C. albicans isolates against 4 antifungals. C. albicans, growing as a biofilm, exhibited resistance to amphotericin B, nystatin, chlorhexidine, and fluconazole, with 50% reduction in metabolic activity (50% RMA) at concentrations of 8, 16, 128, and > 64 µg/mL, respectively. In contrast, planktonically cultured C. albicans were susceptible (50% RMA for the same antifungals was obtained at 0.25, 1.0, 4.0, and 0.5 µg/mL, respectively). In conclusion, results obtained by means of our biofilm model show that biofilm-associated C. albicans cells, compared with cells grown in planktonic form, are resistant to antifungals used to treat denture stomatitis.
Key Words: Candida -associated denture stomatitis • C. albicans biofilm • denture acrylic strips
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Journal of Dental Research, Vol. 80, No. 3,
903-908 (2001)
DOI: 10.1177/00220345010800031101
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