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Journal of Dental Research
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Metabolic Effects of Dental Resin Components in vitro Detected by NMR Spectroscopy

J. Engelmann

'Department of Organic Chemistry, University of Bremen, NW2, FB2, POB 330440, D-28334 Bremen, Germany

G. Leyhausen

Department of Conservative Dentistry and Periodontology, Medical University Hannover, D-30623 Hannover, Germany

D. Leibfritz

'Department of Organic Chemistry, University of Bremen, NW2, FB2, POB 330440, D-28334 Bremen, Germany

W. Geurtsen

Department of Conservative Dentistry and Periodontology, Medical University Hannover, D-30623 Hannover, Germany, geurtsen.werner{at}mh-hannover.de

Earlier studies have shown that the comonomer triethyleneglycol-dimethacrylate (TEGDMA) and the photostabilizer 2-hydroxy-4-methoxybenzophenone (HMBP) are cytotoxic and inhibit cell growth. It was the aim of this study to elucidate the underlying metabolic effects of TEGDMA and HMBP on immortal contact-inhibited Swiss albino mouse embryo cells (3T3 fibroblasts) by nuclear magnetic resonance (NMR) spectroscopy. Cell extracts and culture media were analyzed by NMR spectroscopy for metabolic changes after incubation for 24 hours with ED20-concentrations of TEGDMA and HMBP. TEGDMA could be detected in all fractions (cytosol, lipid fractions, and culture media) of 3T3 cells, while HMBP was found only in the lipid fraction accumulated at a maximum rate (51 nmol/mg DNA) compared with TEGDMA (27 nmol/mg DNA). TEGDMA increased the concentration of phosphomonoesters to 180 ± 36% and decreased the phosphodiesters to 65 ± 5% of controls (control = 100%). Thus, the turnover of phospholipids was enhanced, whereas content and composition of phospholipids of membranes did not alter markedly. Additionally, TEGDMA changed the metabolic state of cells, indicated by slight decreases of nucleoside triphosphates and an increase in the ratio of nucleoside diphosphates to nucleoside triphosphates, while HMBP had no effect. The most remarkable effect of TEGDMA was a nearly complete decline of the intracellular glutathione levels. Analysis of our data shows that NMR spectroscopy of cell-material interactions may reveal metabolic effects of organic test substances which are not detectable by standard in vitro assays. The comonomer TEGDMA affected the metabolism of the cells on different levels, while HMBP accumulated in the lipid fraction and induced significantly fewer effects on cell metabolism.

Key Words: NMR spectroscopy • cell metabolism • TEGDMA • fibroblasts • glutathione.

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Journal of Dental Research, Vol. 80, No. 3, 869-875 (2001)
DOI: 10.1177/00220345010800030501
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