Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Molina, T.
Right arrow Articles by Tomakidi, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Molina, T.
Right arrow Articles by Tomakidi, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Topographic Changes of Focal Adhesion Components and Modulation of p125FAK Activation in Stretched Human Periodontal Ligament Fibroblasts

T. Molina

Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany

K. Kabsch

German Cancer Research Center, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany

A. Alonso

German Cancer Research Center, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany

A. Kohl

Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany

G. Komposch

Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany

P. Tomakidi

Department of Orthodontics and Dentofacial Orthopedics, Dental School, University of Heidelberg, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany, Pascal_Tomakidi{at}med.uni-heidelberg.de

Mechanical stress has been shown in vitro to modulate integrin-β1-mediated activation of p125FAK/FAK. To test the hypothesis whether this also applies to periodontal ligament fibroblasts (PDLs), we subjected human PDLs to mechanical stretch and analyzed stress-induced changes of p125FAK activation by quantitative immunoprecipitation of p125FAK and changes in the topography of molecules localizing in focal adhesions by indirect immunofluorescence. Generally, all components of focal contacts under study-including detection of phosphotyrosine, i.e., integrin-β1, p125FAK, and paxillin-revealed a relative co-localization during stretch application. Under stretch, we observed a re-distribution of all components from the cell periphery to the cytoplasm following the main axes. Tyrosine phosphorylation of p125 FAK was monitored up to 72 hours under stretch. While the amount of p125FAK remained essentially constant, the activation of p125 FAK was clearly modulated. Tyrosine phosphorylation of p125FAK increased from 15 minutes up to 1 hour and declined after stretching periods of 24, 48, and 72 hours. The analysis of our data indicated a stretch-induced re-distribution of focal adhesion components and a modulation of p125 FAK activation, suggesting alterations in focal adhesions and their associated signal cascade.

Key Words: integrins • p125FAK/FAK • paxillin • PDL fibroblasts • stretching

REFERENCES

  • Aplin AE, Howe AK, Juliano RL (1999). Cell adhesion molecules, signal transduction and cell growth. Curr Opin Cell Biol 11:737-744.[CrossRef][Medline] [Order article via Infotrieve]
  • Bellis SL, Perrotta JA, Curtis MS, Turner CE (1997). Adhesion of fibroblasts to fibronectin stimulates both serine and tyrosine phosphorylation of paxillin. Biochem J 325:375-381.[Medline] [Order article via Infotrieve]
  • Bolcato-Bellemin AL, Elkaim R., Abehsera A., Fausser JL, Haikel Y., Tennenbaum H. (2000). Expression of mRNAs encoding for a and P integrin subunits, MMPs, and TIMPs in stretched human periodontal ligament and gingival fibroblasts. J Dent Res 79:1712-1716.
  • Carvalho RS, Scott JE, Yen EH (1995). The effects of mechanical stimulation on the distribution of beta 1 integrin and expression of beta 1-integrin mRNA in TE-85 human osteosarcoma cells. Arch Oral Biol 40:257-264.[CrossRef][Medline] [Order article via Infotrieve]
  • Carvalho RS, Bumann A., Schwarzer C., Scott E., Yen Ehk (1996). A molecular mechanism of integrin regulation from bone cells stimulated by orthodontic forces. Eur J Orthodont 18:227-235.[Abstract/Free Full Text]
  • Chen HC ( 1995). Interaction of focal adhesion kinase with cytoskeletal protein talin. J Biol Chem 270:16995-16999.[Abstract/Free Full Text]
  • Danker K., Gabriel B., Heidrich C., Reutter W. (1998). Focal Adhesion Kinase ppl25FAK and the β1 integrin subunit are constitutively complexed in HaCaT cells. Exp Cell Res 239:326-331.[CrossRef][Medline] [Order article via Infotrieve]
  • Galbraith CG, Sheetz MP (1998). Forces and/or adhesive contacts affect on cell function. Curr Opin Cell Biol 10:566-571.[CrossRef][Medline] [Order article via Infotrieve]
  • Gates RE, King LE Jr, Hanks SK, Nanney LB (1994). Potential role for focal adhesion kinase in migrating and proliferating keratinocytes near epidermal wounds and in culture. Cell Growth Diff 5:891-899.[Abstract]
  • Guan JL, Trevithick JE, Hynes RO (1991). Fibronectin/integrin interaction induces tyrosine phosphorylation of 120-kDa protein. Cell Regul 2:951-964.[Medline] [Order article via Infotrieve]
  • Howard PS, Kucich U., Taliwal R., Korostoff JM (1998). Mechanical forces alter extracellular matrix synthesis by human periodontal ligament fibroblasts. J Periodontal Res 33:500-508.[Medline] [Order article via Infotrieve]
  • Hynes RO (1992). Integrins: versatility modulation and signaling in cell adhesion. Cell 69:11-25.[CrossRef][Medline] [Order article via Infotrieve]
  • Juliano RL, Haskill S. (1993). Signal transduction from the extracellular matrix. J Cell Biol 120:577-585.[Free Full Text]
  • Ni H., Simonsen N., Wilkins JA (1998). Integrin activation by Dithiothreitol or Mn2+ induces a ligand-occupied conformation and exposure of a novel NH2-terminal regulatory site on the β1-integrin chain. J Biol Chem 273:7981-7987.[Abstract/Free Full Text]
  • Ruhl M., Johannsen M., Atkinson J., Manski D., Sahin E., Somasundaram R., et al. (1999). Soluble collagen VI induces tyrosine phosphorylation of paxillin and focal adhesion kinase and activates the MAP kinase erk2 in fibroblasts. Exp Cell Res 250:548-557.[CrossRef][Medline] [Order article via Infotrieve]
  • Sai X., Naruse K., Sokabe M. (1999). Activation of pp60(src) is critical for stretch-induced orienting response in fibroblasts. J Cell Sci 112:1365-1373.[Abstract]
  • Saito M., Saito S., Ngan PW, Shanfeld J., Davidovitch Z. (1991). I1-1β and prostaglandin E are involved in the response of periodontal cells to mechanical stress in vivo and in vitro. Am J Orthod Dentofac Orthop 99:226-240.[Medline] [Order article via Infotrieve]
  • Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT (1992). ppl25FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc Natl Acad Sci USA 89:5192-5196.[Abstract/Free Full Text]
  • Schlaepfer DD, Hauck CR, Sieg DJ ( 1999). Signaling through focal adhesion kinase. Prog Biophys Mol Biol 71:435-478.[CrossRef][Medline] [Order article via Infotrieve]
  • Schwartz MA, Schaller MD, Ginsberg MH (1995). Integrins: emerging paradigms of signal transduction. Annu Rev Cell Dev Biol 11:549-599.[CrossRef][Medline] [Order article via Infotrieve]
  • Sieg DJ, Hauck CR, Ilic D., Klingbeil CK, Schaefer E., Damsky CH, et al. (2000). FAK integrates growth-factor and integrin signals to promote cell migration. Nature Cell Biol 2:249-257.[CrossRef][Medline] [Order article via Infotrieve]
  • Sokabe M., Naruse K., Sai S., Yamasda T., Kawakami K., Inoue M., et al. (1997). Mechanotransduction and intracellular signaling mechanisms of stretch-induced remodeling in endothelial cells. Heart Vessels 12:191-193.[Medline] [Order article via Infotrieve]
  • Yano Y., Geibel J., Sumpio BE (1996). Tyrosine phosphorylation of ppl25FAK and paxillin in aortic endothelial cells induced by mechanical strain. Am J Physiol 271:635-649.

Journal of Dental Research, Vol. 80, No. 11, 1984-1989 (2001)
DOI: 10.1177/00220345010800110701
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Molina, T.
Right arrow Articles by Tomakidi, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Molina, T.
Right arrow Articles by Tomakidi, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?