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Journal of Dental Research
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Reversal of Compromised Bonding to Oxidized Etched Dentin

S.C.N. Lai

'Conservative Dentistry, Faculty of Dentistry, University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, SAR, China

Y.F. Mak

'Conservative Dentistry, Faculty of Dentistry, University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, SAR, China

G.S.P. Cheung

'Conservative Dentistry, Faculty of Dentistry, University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, SAR, China

R. Osorio

Department of Dental Materials, University of Granada, Spain

M. Toledano

Department of Dental Materials, University of Granada, Spain

R.M. Carvalho

Bauru School of Dentistry, University of São Paulo, Brazil

F.R. Tay

'Conservative Dentistry, Faculty of Dentistry, University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Hong Kong, SAR, China, kfctay{at}hknet.com

D.H. Pashley

Department of Oral Biology and Maxillofacial Pathology, School of Dentistry, Medical College of Georgia, Augusta, GA, USA

The mechanism responsible for hydrogenperoxide- or sodium-hypochlorite-induced reductions in dentin bond strength is unknown. This in vitro study tested the hypothesis that these oxidizing agents were responsible by attempting to reverse the effect with sodium ascorbate, a reducing agent. Human dentin was treated with these oxidants before or after being acid-etched and with or without post-treatment with sodium ascorbate. They were bonded with either Single Bond or Excite. Hydrogen peroxide reduced the bond strengths of both adhesives, while sodium hypochlorite produced reduction in adhesion of only Single Bond (p < 0.05). Following treatment with sodium ascorbate, reductions in bond strength were reversed. Transmission and scanning electron microscopy showed partial removal of the demineralized collagen matrix only by sodium hypochlorite. The observed compromised bond strengths cannot be attributed to incomplete deproteinization and may be related to changes in the redox potential of the bonding substrates.

Key Words: sodium ascorbate • sodium hypochlorite • hydrogen peroxide • microtensile bond strength • ultrastructure.

REFERENCES

  • Carr AC, Tijerina T., Frei B. ( 2000). Vitamin C protects against and reverses specific hypochlorous acid- and chloramine-dependent modifications of low-density lipoprotein. Biochem J 346(Pt 2):491-499.
  • Cox CF, Hafez AA, Akimoto N., Otsuki M., Suzuki S., Tarim B. (1998). Biocompatibility of primer, adhesive and resin composite systems on non-exposed and exposed pulps of non-human primate teeth. Am J Dent 11(Spec No):S55-63.[Medline] [Order article via Infotrieve]
  • Daumer KM, Khan AU, Steinbeck MJ (2000). Chlorination of pyridinium compounds. Possible role of hypochlorite, n-chloramines, and chlorine in the oxidation of pyridinoline cross-links of articular cartilage collagen type II during acute inflammation. J Biol Chem 275:34681-34692.[Abstract/Free Full Text]
  • Frankenberger R., Kramer N., Oberschachtsiek H., Petschelt A. (2000). Dentin bond strength and marginal adaption after NaOCI pretreatment. Oper Dent 25:40-45.[Medline] [Order article via Infotrieve]
  • Gutteridge JM (1994). Biological origin of free radicals, and mechanisms of antioxidant protection. Chem Biol Interact 91:133-140.[CrossRef][Medline] [Order article via Infotrieve]
  • Haak R., Wicht MJ, Noack MJ (2000). Does chemomechanical caries removal affect dentine adhesion? Eur J Oral Sci 108:449-455.[Medline] [Order article via Infotrieve]
  • Hawkins CL, Davies MJ (1997). Oxidative damage to collagen and related substrates by metal ion/hydrogen peroxide systems: random attack or site-specific damage? Biochem Biophys Acta 1360:84-96.[Medline] [Order article via Infotrieve]
  • Hawkins CL, Davies MJ (1998a). Hypochlorite-induced damage to proteins: formation of nitrogen-centred radicals from lysine residues and their role in protein fragmentation. Biochem J 332(Pt 3):617-625.
  • Hawkins CL, Davies MJ (1998b). Degradation of hyaluronic acid, poly- and monosaccharides, and model compounds by hypochlorite: evidence for radical intermediates and fragmentation. Free Radic Biol Med 24:1396-1410.[CrossRef][Medline] [Order article via Infotrieve]
  • Hawkins CL, Davies MJ (1999). Hypochlorite-induced oxidation of proteins in plasma: formation of chloramines and nitrogen-centred radicals and their role in protein fragmentation. Biochem J 340(Pt 2):539-548.
  • Heling I., Chandler NP (1998). Antimicrobial effect of irrigant combinations within dentinal tubules. Int Endod J 31:8-14.[Medline] [Order article via Infotrieve]
  • Ikemura K., Shoda K., Endo T. ( 1998). An ATR-FTIR spectroscopic study on the chemical interaction of a new monomer bearing carboxylic moiety with dentin apatite and collagen. J Adhes Soc Jpn 35:9-20 (in Japanese).
  • Inai N., Kanemura N., Tagami J., Watanabe LG, Marshall SJ, Marshall GW (1998). Adhesion between collagen depleted dentin and dentin adhesives. Am J Dent 11:123-127.[Medline] [Order article via Infotrieve]
  • Kato Y., Uchida K., Kawakishi S. (1992). Oxidative fragmentation of collagen and prolyl peptide by Cu(II)/H2O2. Conversion of proline residue to 2-pyrrolidone. J Biol Chem 267:23646-23651.[Abstract/Free Full Text]
  • Komsa-Penkova R., Koynova R., Kostov G., Tenchov B. (2000). Discrete reduction of type I collagen thermal stability upon oxidation. Biophys Chem 83:185-195.[Medline] [Order article via Infotrieve]
  • Nikaido T., Takano Y., Sasafuchi Y., Burrow MF, Tagami J. (1999). Bond strengths to endodontically-treated teeth. Am J Dent 12:177-180.[Medline] [Order article via Infotrieve]
  • Nordbø H., Brown G., Tjan AH ( 1996). Chemical treatment of cavity walls following manual excavation of carious dentin. Am J Dent 9:67-71.[Medline] [Order article via Infotrieve]
  • Perdigao J., Lopes M., Geraldeli S., Lopes GC, Garcia-Godoy F. (2000). Effect of a sodium hypochlorite gel on dentin bonding. Dent Mater 16:311-323.[Medline] [Order article via Infotrieve]
  • Pioch T., Kobaslija S., Schagen B., Gotz H. ( 1999). Interfacial micromorphology and tensile bond strength of dentin bonding systems after NaOCI treatment. J Adhes Dent 1:135-142.[Medline] [Order article via Infotrieve]
  • Prati C., Chersoni S., Pashley DH (1999). Effect of removal of surface collagen fibrils on resin-dentin bonding. Dent Mater 15:323-321.[Medline] [Order article via Infotrieve]
  • Rose RC, Bode AM ( 1993). Biology of free radical scavengers: an evaluation of ascorbate. FASEB J 7:1135-1142.[Abstract]
  • Rueggeberg FA, Margeson DH (1990). The effect of oxygen inhibition on an unfilled/filled composite system. J Dent Res 69:1652-1658.
  • Sano H., Shono T., Sonoda H., Takatsu T., Ciucchi B., Carvalho RM, et al. (1994). Relationship between surface area for adhesion and tensile bond strength-evaluation of a micro-tensile bond test. Dent Mater 10:236-240.[Medline] [Order article via Infotrieve]
  • Schiller J., Arnhold J., Zachaus A., Arnold K. (1997). Reaction of hypochlorous acid with bovine nasal cartilage comparison to pig articular cartilage. Z Naturforsch 52[C]:694-701.
  • Smit MJ, Anderson R. (1992). Biochemical mechanisms of hydrogen peroxide- and hypochlorous acid-mediated inhibition of human mononuclear leukocyte functions in vitro: protection and reversal by anti-oxidants. Agents Actions 36:58-65.[CrossRef][Medline] [Order article via Infotrieve]
  • Tay FR, Moulding KM, Pashley DH (1999). Distribution of nanofillers from a simplified-step adhesive in acid-conditioned dentin. J Adhes Dent 2:103-117.
  • Titley KC, Torneck CD, Ruse ND, Krmec D. (1993). Adhesion of a resin composite to bleached and unbleached human enamel. J Endod 19:112-115.[Medline] [Order article via Infotrieve]
  • Torneck CD, Titley KC, Smith DC, Adibfar A. (1990). Adhesion of light-cured composite resin to bleached and unbleached bovine dentin. Endod Dent Traumatol 6:97-103.[Medline] [Order article via Infotrieve]
  • VanDuijn MM, Tijssen K., VanSteveninck J., Van Den Broek PJ, Van Der Zee J. (2000). Erythrocytes reduce extracellular ascorbate free radicals using intracellular ascorbate as an electron donor. J Biol Chem 275:27720-27725.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 80, No. 10, 1919-1924 (2001)
DOI: 10.1177/00220345010800101101
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This Article
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