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Expression and Regulation of MMP-20 in Human Tongue Carcinoma Cells
A. Väänänen
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, PO Box 5281, FIN-90014 Oulu, Finland
R. Srinivas
Faculty of Medicine, University of Helsinki, Helsinki, Finland
M. Parikka
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, PO Box 5281, FIN-90014 Oulu, Finland
H. Palosaari
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, PO Box 5281, FIN-90014 Oulu, Finland
J.D. Bartlett
Department of Biomineralization & Harvard-Forsyth Department of Oral Biology, Forsyth Institute, Boston, MA, USA
K. Iwata
Biopharmaceutical Department, Fuji Chemical Industries Ltd., Chokeiji, Takaoka, Toyama, Japan
R. Grenman
Department of Otolaryngology, Turku University Hospital, Turku, Finland
U.-H. Stenman
Department of Clinical Chemistry, Helsinki University Hospital, Helsinki, Finland
T. Sorsa
Faculty of Medicine, University of Helsinki, Helsinki, Finland
T. Salo
Department of Diagnostics and Oral Medicine, Institute of Dentistry, University of Oulu, PO Box 5281, FIN-90014 Oulu, Finland, Oulu University Hospital, Oulu, Finland, Tuula.Salo{at}oulu.fi
Human matrix metalloproteinase-20 (MMP-20, enamelysin) fragments the enamel-specific protein amelogenin and has been shown to be synthesized exclusively by odontoblasts and ameloblasts and in certain odontogenic tumors. Here we demonstrate, for the first time, the expression of MMP-20 mRNA and protein in two carcinoma cell lines originating from the tongue. Treatment of the SCC-25 and HSC-3 cells with phorbol 12-myristate 13-acetate (10 nmol/L) up-regulated MMP-20 mRNA and protein expression by up to 1.6-fold, but transforming growth factor beta (10 ng/mL) had no effect. The latent proform of recombinant (r) human MMP-20 was converted by tumor-related trypsin-2. Activated rMMP-20 did not degrade type I or type II collagen, but efficiently hydrolyzed fibronectin, type IV collagen, laminin-1 and -5, tenascin-C, and (3-casein. This implies that MMP-20 not only participates in dental matrix remodeling but is also present in tongue carcinoma cells.
Key Words: matrix metalloproteinases • enamelysin • tongue carcinoma • trypsin-2.
REFERENCES
- Bartlett JD, Simmer JP, Xue J., Margolis HC, Moreno EC (1996). Molecular cloning and mRNA tissue distribution of a novel matrix metalloproteinase isolated from porcine enamel organ. Gene 183:123-128.[CrossRef][Medline]
[Order article via Infotrieve]
- Birkedal-Hansen H., Moore WG, Bodden MK, Windsor LJ, Birkedal-Hansen B., DeCarlo A., et al. (1993). Matrix metalloproteinases: a review. Crit Rev Oral Biol Med 4:197-250.[Abstract/Free Full Text]
- Caterina J., Shi J., Sun X., Qian Q., Yamada S., Liu Y., et al. (2000). Cloning, characterization and expression analysis of mouse enamelysin. J Dent Res 79:1697-1703.
- DeCarlo AA Jr, Windsor LJ, Bodden MK, Harber GJ, Birkedal-Hansen B., Birkedal-Hansen H. (1997). Activation and novel processing of matrix metalloproteinases by a thiol-proteinase from the oral anaerobe Porphyromonas gingivalis. J Dent Res 76:1260-1270.
- Dionisi HM, Checa SK, Viale AM (1995). Protein immunoblotting of stained gels. Biotechniques 19:348-350.[Medline]
[Order article via Infotrieve]
- Fife RS, Sledge GW Jr (1998). Effects of doxycycline on cancer cells in vitro and in vivo. Adv Dent Res 12:94-96.[Free Full Text]
- Fukae M., Tanabe T., Uchida T., Lee SK, Ryu OH, Murakami C., et al. (1998). Enamelysin (matrix metalloproteinase-20): localization in the developing tooth and effects of pH and calcium on amelogenin hydrolysis. J Dent Res 77:1580-1588.
- Grant GM, Giambemardi TA, Grant AM, Klebe RJ (1999). Overview of expression of matrix metalloproteinases (MMP-17, MMP-18, and MMP-20) in cultured human cells. Matrix Biol 18:145-148.[CrossRef][Medline]
[Order article via Infotrieve]
- Hamalainen ER, Kemppainen R., Kuivaniemi H., Tromp G., Vaheri A., Pihlajaniemi T., et al. (1995). Quantitative polymerase chain reaction of lysyl oxidase mRNA in malignantly transformed human cell lines demonstrates that their low lysyl oxidase activity is due to low quantities of its mRNA and low levels of transcription of the respective gene. J Biol Chem 270:21590-21593.[Abstract/Free Full Text]
- Hanemaaijer R., Sorsa T., Konttinen YT, Ding Y., Sutinen M., Visser H., et al. (1997). Matrix metalloproteinase-8 is expressed in rheumatoid synovial fibroblasts and endothelial cells. Regulation by tumor necrosis factor-alpha and doxycycline. J Biol Chem 272:31504-31509.[Abstract/Free Full Text]
- Juarez J., Clayman G., Nakajima M., Tanabe KK, Saya H., Nicolson GL, et al. (1993). Role and regulation of expression of 92-kDa type-IV collagenase (MMP-9) in 2 invasive squamous-cell-carcinoma cell lines of the oral cavity. Int J Cancer 55:10-18.[Medline]
[Order article via Infotrieve]
- Koivunen E., Huhtala M-L., Stenman U-H (1989). Human ovarian tumor-associated trypsin. Its purification and characterization from mucinous cyst fluid and identification as an activator of pro-urokinase. J Biol Chem 264:14095-14099.[Abstract/Free Full Text]
- Koivunen E., Saksela 0, Itkonen 0, Osman S., Huhtala M-L., Stenman U-H. (1991). Human colon carcinoma, fibrosarcoma and leukemia cell lines produce tumor-associated trypsinogen. Int J Cancer 47:592-596.[Medline]
[Order article via Infotrieve]
- Konttinen YT, Lindy 0, Suomalainen K., Ritchlin C., Saari H., Vauhkonen M., et al. (1991). Substrate specificity and activation mechanisms of collagenase from human rheumatoid synovium. Matrix 11:395-403.[Medline]
[Order article via Infotrieve]
- Lewalle JM, Munaut C., Pichot B., Cataldo D., Baramova E., Foidart JM (1995). Plasma membrane-dependent activation of gelatinase A in human vascular endothelial cells. J Cell Physiol 165:475-483.[CrossRef][Medline]
[Order article via Infotrieve]
- Llano E., Pendas AM, Knauper V., Sorsa T., Salo T., Salido E., et al. (1997). Identification and structural and functional characterization of human enamelysin (MMP-20). Biochemistry 36:15101-15108.[Medline]
[Order article via Infotrieve]
- Palosaari H., Wahlgren J., Larmas M., Ronka H., Sorsa T., Salo T., et al. (2000). The expression of MMP-8 in human odontoblasts and dental pulp cells is down-regulated by TGF-β1. J Dent Res 79:77-84.
- Ryu OH, Fincham AG, Hu CC, Zhang C., Qian Q., Bartlett JD, et al. (1999). Characterization of recombinant pig enamelysin activity and cleavage of recombinant pig and mouse amelogenins. J Dent Res 78:743-750.
- Salo T., Lyons JG, Rahemtulla F., Birkedal-Hansen H., Larjava H. (1991). Transforming growth factor-beta 1 up-regulates type IV collagenase expression in cultured human keratinocytes. J Biol Chem 266:11436-11441.[Abstract/Free Full Text]
- Smith GN Jr, Mickler EA, Hasty KA, Brandt KD (1999). Specificity of inhibition of matrix metalloproteinase activity by doxycycline: relationship to structure of the enzyme. Arthritis Rheum 42:1140-1146.[CrossRef][Medline]
[Order article via Infotrieve]
- Sorsa T., Ding Y., Salo T., Lauhio A., Teronen 0, Ingman T., et al. (1994). Effects of tetracyclines on neutrophil, gingival, and salivary collagenases. A functional and Western-blot assessment with special reference to their cellular sources in periodontal diseases. Ann NYAcad Sci 732:112-131.[Medline]
[Order article via Infotrieve]
- Sorsa T., Salo T., Koivunen E., Tyynela J., Konttinen YT, Bergmann U., et al. (1997). Activation of type IV procollagenases by human tumor-associated trypsin-2. J Biol Chem 272:21067-21074.[Abstract/Free Full Text]
- Stracke JO, Fosang AJ, Last K., Mercuri FA, Pendas AM, Llano E., et al. (2000). Matrix metalloproteinases 19 and 20 cleave aggrecan and cartilage oligomeric matrix protein (COMP). FEBS Lett 478:52-56.[CrossRef][Medline]
[Order article via Infotrieve]
- Takata T., Zhao M., Uchida T., Wang T., Aoki T., Bartlett JD, et al. (2000). Immunohistochemical detection and distribution of enamelysin (MMP-20) in human odontogenic tumors. J Dent Res 79:1608-1613.
- Teronen 0, Laitinen M., Salo T., Hanemaaijer R., Heikkila P., Konttinen YT, et al. (2000). Inhibition of matrix metalloproteinases by bisphosphonates may in part explain their effects in the treatment of multiple myeloma. Blood 96:4006-4007.[Free Full Text]
- Thomas GT, Lewis MP, Speight PM (1999). Matrix metalloproteinases and oral cancer. Oral Oncol 35:227-233.[CrossRef][Medline]
[Order article via Infotrieve]
- Uitto VJ, Firth JD, Nip L., Golub LM (1994). Doxycycline and chemically modified tetracyclines inhibit gelatinase A (MMP-2) gene expression in human skin keratinocytes. Ann NY Acad Sci 732:140-151.[Medline]
[Order article via Infotrieve]
- Uria JA, Jimenez MG, Balbin M., Freije Jmp, Lopez-Otin C. (1998). Differential effects of transforming growth factor-beta on the expression of collagenase-1 and collagenase-3 in human fibroblasts. J Biol Chem 273:9769-9777.[Abstract/Free Full Text]
- Xie B., Bucana CD, Fidler IJ (1994). Density-dependent induction of 92-kd type IV collagenase activity in cultures of A431 human epidermoid carcinoma cells. Am J Pathol 144:1058-1067.[Abstract]
Journal of Dental Research, Vol. 80, No. 10,
1884-1889 (2001)
DOI: 10.1177/00220345010800100501
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