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New in vitro Model for the Acquired Enamel Pellicle: Pellicles Formed from Whole Saliva Show Inter-subject Consistency in Protein Composition and Proteolytic Fragmentation Patterns
M.S. Lamkin
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118
D. Migliari
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118
Y. Yao
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118
R.F. Troxler
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118, Department of Biochemistry, Boston University School of Medicine, 80 E. Concord Street, Boston, MA 02118
F.G. Oppenheim
Department of Periodontology and Oral Biology, Boston University Goldman School of Dental Medicine, 700 Albany Street, Boston, MA 02118, Department of Biochemistry, Boston University School of Medicine, 80 E. Concord Street, Boston, MA 02118, fropp{at}bu.edu
The present investigation was undertaken to investigate the variability of proteins in whole saliva which adsorb to hydroxyapatite and are thus likely to be precursors of the acquired enamel pellicle. Whole-saliva proteins from 18 subjects were absorbed to hydroxyapatite, and the gel filtration patterns of released proteins revealed four major peaks and three minor peaks eluting between the major peaks. Amino acid analysis indicated that minor peaks contained fragments of proteins in major peaks, and this was confirmed by sequence analysis. Major peaks comprised 95% and minor peaks comprised 5% of protein absorbed to hydroxyapatite, suggesting a limited proteolytic capacity of whole saliva. HPLC elution patterns of components in minor peaks suggested that proteolysis is not totally random but is an orderly and consistent process. These studies suggest that whole saliva may be suitable as a model system for the investigation of post-secretory modifications of salivary proteins important for the formation of the acquired enamel pellicle.
Key Words: pellicle • whole saliva • adsorption • hydroxyapatite • proteolysis
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Journal of Dental Research, Vol. 80, No. 1,
385-388 (2001)
DOI: 10.1177/00220345010800011501
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Y. Yao, E. A. Berg, C. E. Costello, R. F. Troxler, and F. G. Oppenheim
Identification of Protein Components in Human Acquired Enamel Pellicle and Whole Saliva Using Novel Proteomics Approaches
J. Biol. Chem.,
February 7, 2003;
278(7):
5300 - 5308.
[Abstract]
[Full Text]
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