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Incipient Analysis of Mesenchymal Stem-cell-derived Osteogenesis

Dental Research Center, University of North Carolina School of Dentistry, 404 Brauer Hall CB7450, Chapel Hill, NC 27599-7450

C.T. Harris

Dental Research Center, University of North Carolina School of Dentistry, 404 Brauer Hall CB7450, Chapel Hill, NC 27599-7450

S.P. Bruder

Osiris Therapeutics, Inc., Baltimore, MD 21231

R. Kowalski

Osiris Therapeutics, Inc., Baltimore, MD 21231

S. Kadiyala

Osiris Therapeutics, Inc., Baltimore, MD 21231

Tissue regeneration strategies invoke cell-based therapies for effective tissue formation. Current assessment of mesenchymal stem cell (MSC) directed bone regeneration during in vivo assays is dependent on histologic determination of bone formation. It was the aim of this study to determine the relationship between bone sialoprotein (BSP) expression and osteocalcin expression with subsequent osteogenesis occurring in MSC-based implants. RT-PCR assessment of human actin, collagen type I, BSP, and osteocalcin indicated that undifferentiated cells did not express BSP or osteocalcin. Three weeks following implantation, human BSP could be identified in RNAs isolated from the retrieved implants. For every implant from which human BSP cDNA was amplified, parallel implants harvested at 6 weeks demonstrated bone formation at the histologic level. This study confirms that, in the context of the severe combined immunodeficiency disease (SCID) mouse model, culture-expanded, cryopreserved human MSCs have osteogenic potential and demonstrates that implanted cell gene expression can reveal the early onset of bone formation.

Key Words: mesenchymal stem cell • bone regeneration • bone sialoprotein.

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Journal of Dental Research, Vol. 80, No. 1, 314-320 (2001)
DOI: 10.1177/00220345010800010401


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