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Local and Chronic Application of PTH Accelerates Tooth Movement in Rats

Ogo Dental Clinic, 2-19-16, Tsukamoto, Yodogawa, Osaka 532-0026. Japan

S. Matsumoto

Ogo Dental Clinic, 2-19-16, Tsukamoto, Yodogawa, Osaka 532-0026. Japan

Y. Higuchi

Chugai Pharmaceutical Company, 2-1-9, Kyobashi, Chuo-ku, Tokyo, 104, Japan

T. Takano-Yamamoto

Department of Orthodontics, Faculty of Dentistry, Okayama University, 2-5-1, Shikada, Okayama, Okayama, 700-7825, Japan

K. Yamashita

Department of Orthodontics, Faculty of Dentistry, Okayama University, 2-5-1, Shikada, Okayama, Okayama, 700-7825, Japan

K. Kurisu

Department of Oral Anatomy and Developmental Biology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan

M. Iwamoto

Department of Oral Anatomy and Developmental Biology, Faculty of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka, 565-0871, Japan

We previously reported that whereas systemic continuous infusion of parathyroid hormone (PTH) accelerated orthodontic tooth movement, systemic but intermittent injection of PTH did not increase the rate of tooth movement. Analysis of these data suggested that continuous administration of PTH could be applicable for orthodontic therapy. In the present study, we investigated whether local and chronic application of PTH(1-34) would accelerate orthodontic tooth movement. To increase the residence time of PTH in the injected area, we used methylcellulose (MC) gel (2% w/v) for a slow-release formulation of PTH. MC gel containing PTH (PTH-MC) continuously released biologically active PTH into the acceptor medium for more than 72 hrs in vitro. When male rats received a local injection of PTH-MC into the subpcriosteum in the mesio-palatal region of the maxillary first molar (M¹) every other day, M¹ movement, which was mesially drawn by an orthodontic coil spring attached to the maxillary incisors, was accelerated in a dose-dependent manner. PTH-MC injection at 1 µg/400 g body weight caused a 1.6-fold increase in the rate of tooth movement. The acceleration of tooth movement by PTH-MC injection was marked on days 6, 9, and 12. Local injection of PTH dissolved in saline without MC did not significantly accelerate tooth movement on day 6 or later. Histological examination revealed active osteoclastic bone resorption and a widened periodontal space on the compression side of the periodontal tissue in the PTH-MC-injected rats. These results suggest that local injection of PTH in a slow-release formulation is applicable to orthodontic therapy.

Key Words: PTH • local injection • methylcellulose • slow release • orthodontic tooth movement.

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Journal of Dental Research, Vol. 79, No. 9, 1717-1724 (2000)
DOI: 10.1177/00220345000790091301


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Soma, S. Articles by Iwamoto, M. Search for Related Content PubMed PubMed Citation Articles by Soma, S. Articles by Iwamoto, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB PARATHYROID HORMONE Social Bookmarking                         What's this?