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Expression of mRNAs Encoding for and β Integrin Subunits, MMPs, and TIMPs in Stretched Human Periodontal Ligament and Gingival Fibroblasts

Unite INSERM U 424, Federation dc Rcchcrches Odontologiques

R. Elkaim

Parogène, 11, rue Humann, 67085 Strasbourg Cedex, France

A. Abehsera

Unite INSERM U 424, Federation dc Rcchcrches Odontologiques

J.L. Fausser

Unite INSERM U 424, Federation dc Rcchcrches Odontologiques

Y. Haikel

Unite INSERM U 424, Federation dc Rcchcrches Odontologiques

H. Tenenbaum

Unite INSERM U 424, Federation dc Rcchcrches Odontologiques, h.tenenbaum{at}odonto3.u-strasbg-fr

The biological mechanisms of tooth movement result from the cellular responses of connective tissues to exogenous mechanical forces. Among these responses, the degradation of the extracellular matrix takes place, but the identification of the molecular basis as well as the components implicated in this degradation are poorly understood. To contribute to this identification, we subjected human fibroblasts obtained from the periodontal ligament (PDLs) and from the gingiva (HGFs) to a continuous stretch to quantify the mRNAs encoding for various metalloproteinases (MMPs), their tissue inhibitors (TIMPs), and a and β integrin subunits. Both cell lines reacted by inducing the expression of the mRNAs encoding for MMP-1, MMP-2, TIMP-1, and TIMP-2, while other mRNAs did not vary (MTI-MMP, TIMP-3) or were not expressed (MMP-9). PDLs expressed selectively the mRNAs encoding for a4 and av, with no difference measurable under stretching, while the mRNAs encoding for a6 and β1 were increased and the one encoding for a5 was decreased. HGFs increased the mRNAs encoding for 2, 6, β1, and β3 and decreased the one encoding for a3. Analysis of our data indicated that stretched HGFs and PDLs induced the same pattern of mRNAs encoding for MMPs and TIMPs but differed for those encoding various integrin subunits, known to act as protein receptors in mechanotransduction.

Key Words: intcgrins • metalloproteinases • HGFs • PDLs • stretching.

REFERENCES

• Basdra EK, Komposch G., Huber LA, Papavassiliou AG (1996). Mechanically-stretched human periodontal ligament fibroblasts: identifying components of the mechanotransduction cascade. In: Biological mechanisms of tooth movement and craniofacial adaptation. Davidovitch Z, editor. Boston, MA, USA: Harvard Society for the Advancement of Orthodontics, pp. 41-49.
• Binderman I., Zor U., Kaye AM, Shimshoni Z., Harell A., Somjen D. (1988). The transduction of mechanical force into biochemical events in bone cells may involve activation of phospholipase A2. Calcif Tissue Int 42:261-266.[Medline] [Order article via Infotrieve]
• Carano A., Siciliani G. (1996). Effects of continuous and intermittent forces on human fibroblasts in vitro. Eur J Orthod 18:19-26.[Abstract/Free Full Text]
• Carvalho RS, Scott JE, Yen Ehk (1994). Cytoskeletal changes in human osteoblastic-like cells induced by mechanical strain. In: The biological mechanisms of tooth eruption, resorption and replacements by implants. Davidovitch Z, editor. Boston, MA, USA: Harvard Society for the Advancement of Orthodontics, pp. 135-146.
• Carvalho RS, Bumann A., Schwarzer C., Scott E., Yen EH (1996). A molecular mechanism of integrin regulation from bone cells stimulated by orthodontic forces. Eur J Orthod 18:227-235.[Abstract/Free Full Text]
• Corpet F. (1988). Multiple sequence alignment with hierarchical clustering. Nucleic Acids Res 16:10881-10890.[Abstract/Free Full Text]
• Davidovitch Z. (1991). Tooth movement. Crit Rev Oral Biol Med 2:411-450.[Abstract/Free Full Text]
• Domon S., Shimokawa H., Matsumoto Y., Yamaguchi S., Soma K. ( 1999). In situ hybridization for matrix metalloproteinase-1 and cathepsin K in rat root-resorbing tissue induced by tooth movement. Arch Oral Biol 44:907-915.[CrossRef][Medline] [Order article via Infotrieve]
• Fausser JL, Lesot H., Zidan G., Ruch JV ( 1990). Characterization of a mouse monoclonal antibody to vimentin by indirect immunofluorescence microscopy and immunoblotting. J Biol Buccale 18:29-33.[Medline] [Order article via Infotrieve]
• Giancotti FG (1997). Integrin signaling: specificity and control of cell survival and cell cycle progression. Curr Opin Cell Biol 9:691-700.[CrossRef][Medline] [Order article via Infotrieve]
• Jones DB, Nolte H., Scholubbers JG, Turner E., Veltel D. (1991). Biochemical signal transduction of mechanical strain in osteoblast-like cells. Biomaterials 2:101-110.
• MacKenna DA, Dolfi F., Vuori K., Ruoslahti E. (1998). Extracellular signal-regulated kinase and c-Jun NH2-terminal kinase activation by mechanical stretch is integrin-dependent and matrix-specific in rat cardiac fibroblasts. JClin Invest 101:301-310.[Medline] [Order article via Infotrieve]
• Mariotti A. (1993). The extracellular matrix of the periodontium: dynamic and interactive tissues. Periodontol 2000 3:39-63.
• Marshall JF, Nesbitt SA, Helfrich MH, Horton MA, Polakova K., Hart IR ( 1991). Integrin expression in human melanoma cell lines: heterogeneity of vitronectin receptor composition and function. Int J Cancer 49:924-931.[Medline] [Order article via Infotrieve]
• Masaka T., Hayashi J., Ishikawa I. (1999). Soluble CD14-dependent intercellular adhesion molecular-1 induction by Porphyromonas gingivalis lipopolysaccharide in human gingival fibroblasts. J Periodontol 70:772-778.[CrossRef][Medline] [Order article via Infotrieve]
• Miyajima K., Iwata T., Yoshida K., Yamashita Y., Hayakawa T., Iizuka T. (1994). Mechanical stress stimulates collagenolysis of gingival cells. In: The biological mechanisms of tooth eruption, resorption and replacements by implants. Davidovitch Z, editor. Boston, MA, USA: Harvard Society for the Advancement of Orthodontics, pp. 599-606.
• Ragnarsson B., Carr G., Daniel JC (1985). Isolation and growth of human periodontal ligament cells in vitro. J Dent Res 64:1026-1030.
• Redlich M., Shoshan S., Palmon A. (1999). Gingival response to orthodontic force. Am J Orthod Dentofac Orthop 116:152-158.[Medline] [Order article via Infotrieve]
• Reynolds JJ (1996). Collagenases and tissue inhibitors of metalloproteinases: a functional balance in tissue degradation. Oral Dis 2:70-76.[Medline] [Order article via Infotrieve]
• Saito M., Saito S., Ngan PW, Shanfeld J., Davidovitch Z. (1991). Interleukin 1 beta and prostaglandin E are involved in the response of periodontal cells to mechanical stress in vivo and in vitro. Am J Orthod Dentofac Orthop 99:226-240.[Medline] [Order article via Infotrieve]
• Sandy JR, Farndale RW, Meikle MC (1993). Recent advances in understanding mechanically induced bone remodeling and their relevance to orthodontic theory and practice. Am J Orthod Dentofac Orthop 103:212-222.[Medline] [Order article via Infotrieve]
• Schlaepfer DD, Hunter T. (1998). Integrin signaling and tyrosine phosphorylation: just the FAKs? Trends Cell Biol 8:151-157.[CrossRef][Medline] [Order article via Infotrieve]
• Tamkun TW, DeSimone DW, Fonda D., Patel RS, Buck C., Horwitz AF, et al. (1986). Structure of integrin, a glycoprotein involved in transmembrane linkage between fibronectin and actin. Cell 46:271-282.[CrossRef][Medline] [Order article via Infotrieve]
• Werb Z. ( 1997). ECM and cell surface proteolysis: regulating cellular ecology. Cell 91:439-442.[CrossRef][Medline] [Order article via Infotrieve]
• Yamada KM, Miyamoto S. (1995). Integrin transmembrane signaling and cytoskeletal control. Curr Opin Cell Biol 7:681-689.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 79, No. 9, 1712-1716 (2000)
DOI: 10.1177/00220345000790091201


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