|
Sign In to gain access to subscriptions and/or personal tools.
|
Cloning, Characterization, and Expression Analysis of Mouse Enamelysin
J. Catering
Matrix Metalloproteinase Unit, National Institute of Dental and Craniofacial Research, 30 Convent Drive, MSC 4380, Bethesda, MD 20892-4380, USA, jc239h{at}nih.gov
J. Shi
Matrix Metalloproteinase Unit, National Institute of Dental and Craniofacial Research, 30 Convent Drive, MSC 4380, Bethesda, MD 20892-4380, USA
X. Sun
department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio, 78284, USA
Q. Qian
department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio, 78284, USA
S. Yamada
Matrix Metalloproteinase Unit, National Institute of Dental and Craniofacial Research, 30 Convent Drive, MSC 4380, Bethesda, MD 20892-4380, USA
Y. Liu
Craniofacial Development Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
S. Krakora
Matrix Metalloproteinase Unit, National Institute of Dental and Craniofacial Research, 30 Convent Drive, MSC 4380, Bethesda, MD 20892-4380, USA
J.D. Bartlett
Department of Biomineralization, Forsyth Institute, Boston, MA 02115, USA
Y. Yamada
Craniofacial Development Biology and Regeneration Branch, National Institute of Dental and Craniofacial Research, Bethesda, MD 20892, USA
J.A. Engler
department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, 35294, USA
H. Birkedal-Hansen
Matrix Metalloproteinase Unit, National Institute of Dental and Craniofacial Research, 30 Convent Drive, MSC 4380, Bethesda, MD 20892-4380, USA
J.P. Simmer
department of Pediatric Dentistry, University of Texas Health Science Center at San Antonio, 78284, USA
Enamelysin is a recently isolated member of the matrix metalloproteinase (MMP) family of extracellular matrix (ECM)-degrading enzymes. Here we describe the isolation and characterization of the mouse enamelysin cDNA. Expression of mouse enamelysin was detectable only in ameloblasts and odontoblasts of developing teeth. Characterization of mouse enamelysin demonstrated that it is highly conserved in both its sequence content and pattern of expression relative to the porcine, human, and bovine homologues previously described.
Key Words: MMP-20 • amelogenin • ameloblast • odontoblast.
REFERENCES
- a 21 kDa and 25 kDa bovine enamel metalloproteinase. Eur J Oral Sci 106:345-349.
- Fukae M., Tanabe T., Uchida T., Lee SK, Ryu OH, Murakami C., et al. (1998). Enamelysin (matrix metalloproteinase-20): localization in the developing tooth and effects of pH and calcium on amelogenin hydrolysis. J Dent Res 77:1580-1588.
- Llano E., Pendas AM, Knauper V., Sorsa T., Salo T., Salido E., et al. (1997). Identification and structural and functional characterization of human enamelysin (MMP-20). Biochemistry 36:15101-15108.[Medline]
[Order article via Infotrieve]
- Massova I., Kotra LP, Fridman R., Mobashery S. (1998). Matrix metalloproteinases: structures, evolution, and diversification. FASEB J 12:1075-1095.[Abstract/Free Full Text]
- Matrisian LM (1992). The matrix-degrading metalloproteinases. Bioessays 14:455-463.[CrossRef][Medline]
[Order article via Infotrieve]
- Ryu OH, Fincham AG, Hu CC, Zhang C., Qian Q., Bartlett JD, et al. (1999). Characterization of recombinant pig enamelysin activity and cleavage of recombinant pig and mouse amelogenins. J Dent Res 78:743-750.
- Sambrook J., Fritsch EF, Maniatis T. (1989). Molecular cloning-a laboratory manual. 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
- Simmer JP, Fincham AG (1995). Molecular mechanisms of dental enamel formation. Crit Rev Oral Biol Med 6:84-108.[Abstract/Free Full Text]
- Smith CE, Pompura JR, Borenstein S., Fazel A., Nanci A. (1989). Degradation and loss of matrix proteins from developing enamel. Anat Rec 224:292-316.[CrossRef][Medline]
[Order article via Infotrieve]
- Snead ML, Bringas P. Jr, Bessem C., Slavkin HC (1984). De novo gene expression detected by amelogenin gene transcript analysis. Dev Biol 104:255-258.[CrossRef][Medline]
[Order article via Infotrieve]
- Stetler-Stevenson WG, Aznavoorian S., Liotta LA (1993). Tumor cell interactions with the extracellular matrix during invasion and metastasis. Ann Rev Cell Biol 9:541-573.[CrossRef]
- Woessner JF Jr (1991). Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J 5:2145-2154.[Abstract]
- Woessner JF (1998). Role of matrix proteases in processing enamel proteins. Connect Tissue Res 39:373-377.
Journal of Dental Research, Vol. 79, No. 9,
1697-1703 (2000)
DOI: 10.1177/00220345000790091001
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
J-W Kim, J P Simmer, T C Hart, P S Hart, M D Ramaswami, J D Bartlett, and J C-C Hu
MMP-20 mutation in autosomal recessive pigmented hypomaturation amelogenesis imperfecta
J. Med. Genet.,
March 1, 2005;
42(3):
271 - 275.
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Bourd-Boittin, D. Septier, R. Hall, M. Goldberg, and S. Menashi
Immunolocalization of Enamelysin (Matrix Metalloproteinase-20) in the Forming Rat Incisor
J. Histochem. Cytochem.,
April 1, 2004;
52(4):
437 - 445.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Caterina, Z. Skobe, J. Shi, Y. Ding, J. P. Simmer, H. Birkedal-Hansen, and J. D. Bartlett
Enamelysin (Matrix Metalloproteinase 20)-deficient Mice Display an Amelogenesis Imperfecta Phenotype
J. Biol. Chem.,
December 13, 2002;
277(51):
49598 - 49604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
