This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Thotakura, S.R. Articles by George, A. Search for Related Content PubMed PubMed Citation Articles by Thotakura, S.R. Articles by George, A. Social Bookmarking                         What's this?

The Non-collagenous Dentin Matrix Proteins are Involved in Dentinogenesis Imperfecta Type II (DGI-II)

Department of Oral Biology, School of Dentistry, M/C 690, University of Illinois at Chicago, Chicago, IL 60612, USA

T. Mah

Department of Basic and Behavioral Sciences, Northwestern University, Chicago, IL 60611, USA

R. Srinivasan

Department of Oral Biology, School of Dentistry, M/C 690, University of Illinois at Chicago, Chicago, IL 60612, USA

Y. Takagi

Department of Pediatric Dentistry, School of Dentistry, Tokyo Medical and Dental University, 1-5-45, Yushima, Bunkyo-Ku, Tokyo 113-8549, Japan

A. Veis

Department of Basic and Behavioral Sciences, Northwestern University, Chicago, IL 60611, USA

A. George

Department of Oral Biology, School of Dentistry, M/C 690, University of Illinois at Chicago, Chicago, IL 60612, USA

Dentinogenesis Imperfecta type II (DGI-II) is a localized form of mesodermal dysplasia of the dentin affecting both the primary and permanent dentitions. This is an autosomal-dominant disease in which there is a disorder in dentin mineralization. Several studies have localized DGI-II to human chromosome 4 in the region 4q 12-21. Many ECM genes—such as OPN, DMP1, DMP2, DMP3 (DSPP), and BSP—have been mapped to the same locus. Biochemical studies indicated that dentin phosphophoryn (DMP2) might be a candidate gene in DGI-II. In this study, we have used histological and RFLP analyses of tissues from a DGI-II-affected patient, as compared with two normal controls, to determine if DMP1, 2, or 3 was linked to DGI-II. The histology of the affected tooth was very different in the DGI-II patient as compared with the normals. In particular, the dentinal tubules in the DGI-1I patient were very irregular, which could be the result of perturbations in the process of dentin formation. Patient and control DNA samples were digested with EcoRI or Pstl and Southern-hybridized with the DMP1, DMP2, and DMP3 cDNAs. Few differences in the restriction pattern were observed between affected and normal samples for DMP1 and DMP3-3' region (phosphophoryn-like sequences) probes. On the other hand, DMP2 showed a dramatic shift in the restriction pattern in DGI-II. This study suggests that the different restriction enzyme digestion profiles of the DNA from the DGI-II patient, as probed by DMP2, might be related to the defective mineralization of dentin in DGI-II.

Key Words: dentinogenesis imperfecta type II • non-collagenous proteins • dentin matrix proteins and biomineralization

REFERENCES

• Ball SP, Cook PJ, Mars M., Buckton KE (1982). Linkage between dentinogenesis imperfecta and Gc. Ann Hum Genet 46:35-40.[Medline] [Order article via Infotrieve]
• Butler WT (1987). Dentin specific proteins. Meth Enzymol 145:290-303.[Medline] [Order article via Infotrieve]
• Crosby AH, Scherpbier-Heddema T., Wijmenga C., Altherr MR, Murray JC, Buetow KH, et al. (1995a). Genetic mapping of the dentinogenesis imperfecta type II locus. Am J Hum Genet 57:832-839.[Medline] [Order article via Infotrieve]
• Crosby AH, Edwards SJ, Murray JC, Dixon MJ (1995b). Genomic organization of the human osteopontin gene: exclusion of the locus from a causative role in the pathogenesis of dentinogenesis imperfecta type II. Genomics 27:155-160.[CrossRef][Medline] [Order article via Infotrieve]
• D'Souza RN, Cavender A., Sunavala G., Alvarez J., Ohshima T., Kulkami AB, et al. (1997). Gene expression patterns of murine dentin matrix protein 1 (DMP1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. J Bone Miner Res 12:2040-2049.[CrossRef][Medline] [Order article via Infotrieve]
• Dickson IR, Dimuzio MT, Volpin D., Ananthanarayanan S., Veis A. ( 1975). The extraction of phosphoproteins from bovine dentin. Calcif Tissue Res 19:51-61.[Medline] [Order article via Infotrieve]
• Feng JQ, Luan X., Wallace J., Jing D., Ohshima T., Kulkami AB, et al. (1998). Genomic organization, chromosomal mapping and promoter analysis of the mouse dentin sialophosphoprotein (DSPP) gene, which codes for both dentin sialoprotein and dentin phosphoprotein. J Biol Chem 273:9457-9464.[Abstract/Free Full Text]
• George A., Sabsay B., Simonian PA, Veis A. ( 1993). Characterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralization. J Biol Chem 268:12624-12630.[Abstract/Free Full Text]
• George A., Bannon L., Sabsay B., Dillon JW, Malone J., Veis A., et al. (1996). The carboxyl-terminal domain of phosphophoryn contains unique extended triplet amino acid repeat sequences forming ordered carboxyl-phosphate interaction ridges that may be essential in the biomineralization process. J Biol Chem 271:32869-32873.[Abstract/Free Full Text]
• George A., Srinivasan R., Thotakura SR, Liu K., Veis A. ( 1999). Rat dentin matrix protein 3 (DMP3) is a compound protein of rat dentin sialoprotein (DSP) and phosphophryn (PP). Connect Tissue Res 40:49-57.[Medline] [Order article via Infotrieve]
• Hirst KL, Simmons D., Feng J., Aplin H., Dixon MJ, MacDougall M. (1997). Elucidation of the sequence and the genomic organization of the human dentin matrix acidic phosphoprotein 1 (DMP1) gene: exclusion of the locus from the causative role in the pathogenesis of dentinogenesis imperfecta type II. Genomics 42:38-45.[CrossRef][Medline] [Order article via Infotrieve]
• Hursey RJ Jr, Witkop CJ Jr, Miklashek D., Sackett LM (1956). Dentinogenesis imperfecta in a racial isolate with multiple hereditary defects. Oral Surg 9:641-658.
• Kocher MS, Shapiro F. (1998). Osteogenesis imperfecta. J Am Acad Orthop Surg 6:225-236.[Abstract]
• Komorowska A., Rozynkowa D., Lee KW, Renouf DV, Nicholls AC, MacKenzie J., et al. (1989). A Polish variant of isolated dentinogenesis imperfecta with a generalised connective tissue defect. Br Dent J 167:239-243.[Medline] [Order article via Infotrieve]
• MacDougall M., Zeichner-David M., Murray J., Crall N., Davis A., Slavkin H. (1992). Dentin phosphoprotein gene locus is not associated with dentinogenesis imperfecta types II and III. Am J Hum Genet 50:190-194.[Medline] [Order article via Infotrieve]
• MacDougall M., Simmons D., Luan X., Nydegger J., Feng J., Gu TT (1997). Dentin phosphoprotein and dentin sialoprotein are cleavage products expressed from a single transcript coded by a gene on human chromosome 4. Dentin phosphoprotein DNA sequence determination. J Biol Chem 272:835-842.[Abstract/Free Full Text]
• Ritchie HH, Wang LH ( 1996). Sequence determination of an extremely acidic rat dentin phosphoprotein. J Biol Chem 271:21695-21698.[Abstract/Free Full Text]
• Shields ED, Bixler D., el-Kafrawy AM (1973). A proposed classification for heritable human dentine defects with a description of a new entity. Arch Oral Biol 18:543-553.[CrossRef][Medline] [Order article via Infotrieve]
• Takagi Y., Sasaki S. (1986). Histological distribution of phosphophoryn in normal and pathological human dentins. J Oral Pathol 15:463-467.[Medline] [Order article via Infotrieve]
• Takagi Y., Sasaki S. (1988). A probable common disturbance in the early stage of odontoblast differentiation in dentinogenesis imperfecta type I and type II. J Oral Pathol 17:208-212.[Medline] [Order article via Infotrieve]
• Takagi Y., Veis A. ( 1981). Matrix protein differences between human normal and dentinogenesis imperfecta dentin. In: The chemistry and biology of mineralized connective tissues. Veis A, editor. New York: Elsevier /North-Holland, pp. 233-243.
• Takagi Y., Veis A., Sauk JJ ( 1983). Relation of mineralization defects in collagen matrices to noncollagenous protein components. Identification of a molecular defect in dentinogenesis imperfecta. Clin Orthop 176:282-290.
• Veis A., Perry A. (1967). The phosphoprotein of the dentin matrix. Biochemistry 6:2409-2416.[Medline] [Order article via Infotrieve]
• Vikkula M., Metsaranta M., Syvanen AC, Ala-Kokko L., Vuorio E., Peltonen L. (1992). Structural analysis of the regulatory elements of the type-II procollagen gene. Conservation of promoter and first intron sequences between human and mouse. Biochem J 285:287-294.[Medline] [Order article via Infotrieve]
• Witkop CJ (1957). Hereditary defects in enamel and dentin. Acta Genet 7:236-239.
• Wright JT, Gantt DG (1985). The ultrastructure of the dental tissues in dentinogenesis imperfecta in man. Arch Oral Biol 30:201-206.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 79, No. 3, 835-839 (2000)
DOI: 10.1177/00220345000790030901


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K. Narayanan, S. Gajjeraman, A. Ramachandran, J. Hao, and A. George Dentin Matrix Protein 1 Regulates Dentin Sialophosphoprotein Gene Transcription during Early Odontoblast Differentiation J. Biol. Chem., July 14, 2006; 281(28): 19064 - 19071. [Abstract] [Full Text] [PDF]

				B. M. Steiglitz, M. Ayala, K. Narayanan, A. George, and D. S. Greenspan Bone Morphogenetic Protein-1/Tolloid-like Proteinases Process Dentin Matrix Protein-1 J. Biol. Chem., January 9, 2004; 279(2): 980 - 986. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Thotakura, S.R. Articles by George, A. Search for Related Content PubMed PubMed Citation Articles by Thotakura, S.R. Articles by George, A. Social Bookmarking                         What's this?