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Localization of Estrogen-receptor-related Antigen in Human Odontoblasts

Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland

M. Larmas

Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland, Oulu University Hospital, Oulu, Finland

T. Salo

Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland

Estrogen receptors have been demonstrated in many osteogenic cell lines. Recently, we showed that estrogen deficiency induced by ovariectomy caused enhanced dentin formation in adult rats, suggesting that estrogen receptors may be present in dental tissues. Nothing is known about estrogen receptors in human teeth. We used immunohistochemical staining and immuno-blotting to demonstrate the presence of estrogen receptors in human pulp and/or the pulpo-dentinal border. Unerupted human wisdom teeth were surgically removed, frozen in liquid nitrogen, and prepared for immunological studies. Western blot analysis with monoclonal antibodies specific for human estrogen-receptor-related antigens demonstrated an approximately 29-kDa clear double band in the material scraped from the predentin-odontoblast border and in the fluid that emerged into the pulpal chamber, evidently from the odontoblasts. A weaker double band was also present in pulpal tissue samples. By immunohistochemical staining, estrogen-receptor-related antigens were visualized in the predentinal-odontoblast region and in the pulpal blood vessels. Our results suggest the presence of estrogen receptors in human teeth, and thus the previously reported enhancement of the dentin formation in rats after ovariectomy may be mediated via these receptors.

Key Words: estrogen receptor • pulp • odontoblast • Western blot

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Journal of Dental Research, Vol. 77, No. 6, 1384-1387 (1998)
DOI: 10.1177/00220345980770060201


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hietala, E.-L. Articles by Salo, T. Search for Related Content PubMed PubMed Citation Articles by Hietala, E.-L. Articles by Salo, T. Social Bookmarking                         What's this?