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"In vitro" Dissolution of Coral in Peritoneal or Fibroblast Cell Cultures

INSERM-U443 - 146, Rue Leo Saignat, Universite de Bordeaux , 33076 Bordeaux, France

R. Bareille

INSERM-U443 - 146, Rue Leo Saignat, Universite de Bordeaux , 33076 Bordeaux, France

F. Rouais

INSERM-U443 - 146, Rue Leo Saignat, Universite de Bordeaux , 33076 Bordeaux, France

B. Basse-Cathalinat

INSERM-U443 - 146, Rue Leo Saignat, Universite de Bordeaux , 33076 Bordeaux, France

B. Dupuy

INSERM-U443 - 146, Rue Leo Saignat, Universite de Bordeaux , 33076 Bordeaux, France

Previous studies have shown that in vivo coral resorption involves a biphasic process: First, the edges of the coral block become powdery, then extracellular fluid and phagocytosis contribute to the dissolution of the crystals. The authors examined some types of cells that could be involved in phagocytosis, particularly the ability of both dermal fibroblasts and mouse-resident peritoneal cells to phagocytose and dissolve coral powder "in vitro". Radioactive coral was incubated for 24, 48, or 72 hrs with cells in the presence or absence of cytochalasin B (a phagocytic inhibitor) or chloroquine (a lysosomotropic agent). Furthermore, to specify the role of crystal cell contacts in the solubilization process, they incubated radioactive coral in conditioned media (obtained from two-day human fibroblastic or macrophagic cell culture in the presence or absence of non-radioactive coral) or at a distance from the cells using culture inserts. Measurements of the radioactivity in the different supernatants were performed. Transmission electron microscopy was carried out on the cells cultivated in the presence or absence of radioactive coral. The data suggest that both fibroblasts and macrophages dissolve the coral, and that the intracellular degradation in phagolysosomes is one of the mechanisms explaining coral powder dissolution.

Key Words: coral dissolution • phagocytosis • cell culture.

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Journal of Dental Research, Vol. 77, No. 2, 406-411 (1998)
DOI: 10.1177/00220345980770020901


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Fricain, J.-Ch. Articles by Dupuy, B. Search for Related Content PubMed PubMed Citation Articles by Fricain, J.-Ch. Articles by Dupuy, B. Social Bookmarking                         What's this?