Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Oates, T.W.
Right arrow Articles by Cochran, D.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oates, T.W.
Right arrow Articles by Cochran, D.L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

PDGF-{alpha} Receptor Subunit Expression Down-regulated by IL-1β in Human Periodontal Ligament Cells

T.W. Oates

Department of Periodontics, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284

J.-E. Xie

Division of Oral Biology, Boston University School of Graduate Dentistry

S. Clinton

Department of Periodontics, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284

A.M. Hoang

Department of Periodontics, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284

D.T. Graves

Division of Oral Biology, Boston University School of Graduate Dentistry, Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts 02118

D.L. Cochran

Department of Periodontics, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78284

The responses of cells to the distinct PDGF isoforms have been correlated directly to the relative numbers of specific PDGF receptor subunits on the cell surface. The modulation of PDGF-a receptor subunits, the major subunit expressed in human periodontal ligament (PDL) cells, by cytokines present in the periodontal wound site, such as interleukin-1 (IL-1), may be an important factor influencing regenerative outcomes. The purpose of the present study was to examine the effects of IL-1β on PDGF-a receptor subunit expression in human PDL cells. Primary cultures of human PDL cells were treated with IL-1β over a range of concentrations. We assessed PDGF-a receptor subunits by examining the mitogenic responses of cells to PDGF-AA, specific binding of 125I-labeled PDGF-AA, immunofluorescent analysis of PDGF-a receptor subunits, and PDGF-a receptor subunit mRNA levels using Northern blot analysis. The results demonstrate a significant concentration-dependent decrease in 3H-thymidine incorporation in response to PDGF-AA following IL-1β treatment (p < 0.001). This decreased response correlated directly with IL-1-induced decreases in 125I-labeled PDGF-AA binding (p < 0.01), the numbers of immunolabeled PDGF-a receptor subunits, and in PDGF-a receptor subunit mRNA levels. However, when combined with TGF-β, IL-1β did not show additional down-regulation in proliferative response to PDGF-AA or PDGF-a receptor subunits beyond that achieved with these factors individually. These experiments identify IL-1 (3, along with TGF-β, as significant inhibitors of PDGF stimulation in human PDL cells, acting through the down-regulation of PDGF-a receptor subunit expression.

Key Words: periodontal ligament cells • PDGF receptors • interleukin-1.

REFERENCES

  • Blanco FJ, Geng Y., Lotz M. ( 1995). Differentiation-dependent effects of IL-1 and TGF-beta on human articular chondrocyte proliferation are related to inducible nitric oxide synthase expression. J Immunol 154:4018-4026.[Abstract]
  • Bolander ME (1992). Regulation of fracture repair by growth factors. Proc Soc Exp Biol Med 200:165-170.[CrossRef][Medline] [Order article via Infotrieve]
  • Boyce BF, Aufdemorte TB, Garrett IR, Yates AJ, Mundy GR (1989). Effects of interleukin-1 on bone turnover in normal mice. Endocrinol 125:1142-1150.[Abstract/Free Full Text]
  • Centrella M., McCarthy TL, Kusmik WF, Canalis E. (1992). Isoform-specific regulation of platelet-derived growth factor activity and binding in osteoblast-enriched cultures from fetal rat bone. J Clin Invest 89:1076-1084.[Medline] [Order article via Infotrieve]
  • Cho MI, Lin W.-L, Genco RJ (1995). Platelet-derived growth factor-modulated guided tissue regenerative therapy. J Periodontol 66:522-530.[Medline] [Order article via Infotrieve]
  • Chomczynski P., Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 162:156-159.[Medline] [Order article via Infotrieve]
  • Collins JM, Grogan WM (1989). Comparison between flow cytometry and fluorometry for the kinetic measurement of membrane fluidity parameters. Cytometry 10:44-49.[Medline] [Order article via Infotrieve]
  • Giannobile WV, Finkelman RD, Lynch SE (1994). Comparison of canine and non-human primate animal models for periodontal regenerative therapy: Results following a single administration of PDGF/IGF-I. J Periodontol 65:1158-1168.[Medline] [Order article via Infotrieve]
  • Gilardetti RS, Chaibi MS, Stroumza J., Williams SR, Antoniades HN, Carnes DC, et al. (1991). High-affinity binding of PDGF-AA and PDGF-BB to normal human osteoblastic cells and modulation by interleukin-1. Am J Physiol 261:C980-C985.
  • Graves DT, Cochran DL (1990). Mesenchymal cell growth factors. Crit Rev Oral Biol Med 1:17-36.[Free Full Text]
  • Heldin CH, Backstrom G., Ostman A., Hammacher A., Ronnstrand L., Rubin K., et al. (1988). Binding of different dimeric forms of PDGF to human fibroblasts: Evidence for two separate receptor types. EMBO J 7:1387-1397.[Medline] [Order article via Infotrieve]
  • Jandinski JJ, Stashenko P., Feder LS, Leung CC, Peros WJ, Rynar JE, et al. (1991). Localization of interleukin-1β in human periodontal tissue. J Periodontol 62:36-43.[Medline] [Order article via Infotrieve]
  • Kose KN, Xie JF, Carnes DL, Graves DT (1996). Pro-inflammatory cytokines down regulate platelet-derived growth factor-a receptor gene expression in human osteoblastic cells. J Cell Physiol 166:188-197.[CrossRef][Medline] [Order article via Infotrieve]
  • Lynch SE, Williams RC, Polson AM, Howell TH, Reddy MS, Zappa VE, et al. (1989). A combination of platelet-derived and insulin-like growth factors enhance periodontal regeneration. J Clin Periodontol 16:545-548.[CrossRef][Medline] [Order article via Infotrieve]
  • Lynch SE, deCastilla GR, Williams RC, Kiritsy CP, Howell TH, Reddy MS, et al. (1991). The effects of short-term application of a combination of platelet-derived and insulin-like growth factors on periodontal wound healing. J Periodontol 62:458-467.[Medline] [Order article via Infotrieve]
  • Majack RA, Grieshaber NA, Cook CL, Weiser MC, McFall RC, Grieshaber SS, et al. (1996). Smooth muscle cells isolated from the neointima after vascular injury exhibit altered responses to platelet-derived growth factor and other stimuli. J Cell Physiol 167:106-112.[CrossRef][Medline] [Order article via Infotrieve]
  • Matsuda N., Lin WL, Kumar NM, Cho MI, Genco RJ (1992). Mitogenic, chemotactic, and synthetic responses of rat periodontal ligament fibroblastic cells to polypeptide growth factors in vitro. J Periodontol 63:515-525.[Medline] [Order article via Infotrieve]
  • Matsui T., Heideran M., Miki T., Popescu N., La Rochelle W., Kraus M., et al. (1989). Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes. Science 243:800-802.[Abstract/Free Full Text]
  • Mundy GR (1993). Cytokines and growth factors in the regulation of bone remodeling. J Bone Miner Res 8:S505-S510.[Medline] [Order article via Infotrieve]
  • Oates TW, Rouse CA, Cochran DL (1993). Mitogenic effects of growth factors on human periodontal ligament cells in vitro. J Periodontol 64:142-148.[Medline] [Order article via Infotrieve]
  • Oates TW, Kose KN, Xie JF, Graves DT, Collins JM, Cochran DL (1995). Receptor binding of PDGF-AA and PDGF-BB, and the modulation of PDGF receptors by TGF-β, in human periodontal ligament cells. J Cell Physiol 162:359-366.[Medline] [Order article via Infotrieve]
  • Park J.-B, Matsuura M., Han K.-Y. Norderyd 0, Lin W.-L, Genco RJ, et al. (1995). Periodontal regeneration in class III furcation defects of beagle dogs using guided tissue regenerative therapy with platelet-derived growth factor. J Periodontol 66:462-477.[Medline] [Order article via Infotrieve]
  • Rutherford RB, Niekrash CE, Kennedy JE, Charette MF (1992). Platelet-derived and insulin-like growth factors stimulate regeneration of periodontal attachment in monkeys. J Periodont Res 27:285-290.[CrossRef][Medline] [Order article via Infotrieve]
  • Rutherford RB, Ryan ME, Kennedy JE, Tucker MM, Charette MF (1993). Platelet-derived growth factor and dexamethasone combined with a collagen matrix induce regeneration of the periodontium in monkeys. J Clin Periodontol 20:537-544.[CrossRef][Medline] [Order article via Infotrieve]
  • Seifert RA, Hart CE, Phillips PE, Forstrom JW, Ross R., Murray MJ, et al. (1989). Two different subunits associate to create isoform-specific platelet-derived growth factor receptors. J Biol Chem 264:8771-8778.[Abstract/Free Full Text]
  • Seifert RA, vanKoppen A., Bowen-Pope DF (1993). PDGF-AB requires PDGF receptor a-subunits for high-affinity, but not for low affinity, binding and signal transduction. J Biol Chem 268:4473-4480.[Abstract/Free Full Text]
  • Somerman MJ, Archer SY, Imm GR, Foster RA (1988). A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 67:66-70.
  • Stashenko P., Jandinski JJ, Fujiyoshi P., Rynar J., Socransky SS (1991a). Tissue levels of bone resorptive cytokines in periodontal disease. J Periodontol 62:504-509.[Medline] [Order article via Infotrieve]
  • Stashenko P., Fujiyoshi P., Obernesser MS, Prostak L., Haffajee AD, Socransky SS (1991b). Levels of interleukin-1 beta in tissue from sites of active periodontal disease. J Clin Periodontol 18:548-554.[CrossRef][Medline] [Order article via Infotrieve]
  • Taichman RS, Hauschka PV (1992). Effects of interleukin-1 beta and tumor necrosis factor-alpha on osteoblastic expression of osteocalcin and mineralized extracellular matrix in vitro. Inflammation 16:587-601.[CrossRef][Medline] [Order article via Infotrieve]
  • Takeshita A., Nui ZG, Hanazawa S., Takara I., Higushi H., Katayama I., et al. (1992). Effect of interleukin-1 β on gene expressions and functions of fibro-blastic cells derived from human periodontal ligament. J Periodont Res 27:250-255.[Medline] [Order article via Infotrieve]
  • Tsukamoto T., Matsui T., Nakata H., Ito M., Natazuka T., Fukase M., et al. (1991). Interleukin-1 enhances the response of osteoblasts to platelet-derived growth factor through the alpha receptor-specific up-regulation. J Biol Chem 266:10143-10147.[Abstract/Free Full Text]
  • Wilton JM, Bampton JL, Griffiths GS, Curtis MA, Life JS, Johnson NW, et al. (1992). Interleukin-1 beta (IL-1β) levels in gingival crevicular fluid from adults with previous evidence of destructive periodontitis; a cross sectional study. J Clin Periodontol 19:53-57.[CrossRef][Medline] [Order article via Infotrieve]
  • Xie JF, Stroumza J., Graves DT (1994). 11-1 down-regulates PDGF-a receptor gene expression at the transcriptional level in human osteoblastic cells. J Immunol 153:378-383.[Abstract]

Journal of Dental Research, Vol. 77, No. 10, 1791-1798 (1998)
DOI: 10.1177/00220345980770100601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Oates, T.W.
Right arrow Articles by Cochran, D.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Oates, T.W.
Right arrow Articles by Cochran, D.L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?