Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Carlén, A.
Right arrow Articles by Strömberg, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carlén, A.
Right arrow Articles by Strömberg, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Agglutinin and Acidic Proline-rich Protein Receptor Patterns May Modulate Bacterial Adherence and Colonization on Tooth Surfaces

A. Carlén

Department of Cariology, Faculty of Odontology, Göteborg University, Medicinaregatan 12, S-413 90 Göteborg

P. Bratt

Department of Cariology, Faculty of Odontology, UmeÅ University, S-901 87 UmeÅ, Sweden

C. Stenudd

Department of Cariology, Faculty of Odontology, UmeÅ University, S-901 87 UmeÅ, Sweden

J. Olsson

Department of Cariology, Faculty of Odontology, Göteborg University, Medicinaregatan 12, S-413 90 Göteborg

N. Strömberg

Department of Cariology, Faculty of Odontology, UmeÅ University, S-901 87 UmeÅ, Sweden

Bacterial binding to salivary proteins may in part account for individual differences in the colonization of tooth surface. High-motecular-weight glycoproteins, agglutinins, mediate S. mutans adherence, whereas acidic proline-rich proteins mediate adherence of other early-colonizing streptococci and Actinemyces. The aim of the present study was to examine the composition of adherence-related salivary proteins and dental plaque micro-organisms in three individuals with a low, moderate, and high capacity to mediate S. mutans adherence. The S. mutans (strain Ingbritt) binding activity resided with 300-kDa agglutinin which was six-fold more prevalent in the high S. mutans binding saliva compared with the low one. Binding to all three salivas was completely by a monoclonal anti-agglutinin antibody. The moderate S. mutans binding saliva was found to contain adherence-inhibiting components. Furthermore, the low and moderate S. mutant binding salivas mediated binding of A. naeslundii strain LY7 to a greater extent the saliva with high S. mutans binding. The A. naeslundii binding activity resided with the acidic proline-rich proteins (APRPS) and paralleled the relative content of 106- and 150-residue A-PRPS. Low A. naeslundii binding coincided with an almost two-fold higher ratio of 106/150 APRPs compared the high A. naestundii binding saliva. During conventional gel filtration, a degradation of the acidic, basic, and glycosylated proline-rich proteins was evident in the saliva with high S. mutatis and low A. naeslundii binding. This saliva donor had a comparably high rate of dental plaque formation, high counts of S. mutans, and low counts of other streptococci and Actinomyces.

Key Words: saliva • adherence • acidic proline-rich proteins • agglutinins • S. mutant, A. naeslundii.

REFERENCES

  • Anderson LC, Mandel ID (1982). Salivary protein polymorphisms in caries-resistant adults. J Dent Res 61:1167-1168.
  • Anderson LC, Lamberts BL, Bruton WF (1982). Salivary protein polymorphisms in caries-free and caries-active adults. J Dent Res 61:393-396.
  • Azen EA ( 1993). Genetics of salivary protein polymorphisms. Crit Rev Oral Biol Med 4:479-485.[Abstract/Free Full Text]
  • Azen EA, Denniston C. (1981). Genetic polymorphism of PIF (Parotid Isoelectric Focusing Variant) proteins with linkage to the PPP (Parotid Proline-Rich Protein) gene complex. Biochem Genet 19:475-485.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen EA, Yu PL (1984). Genetic polymorphisms of Pe and Po salivary proteins with probable linkage of their genes to the salivary protein gene complex (SPC). Biochem Genet 22:1065-1080.[CrossRef][Medline] [Order article via Infotrieve]
  • Azen E., Prakobphol A., Fisher SJ (1993). PRB3 null mutations result in absence of the proline-rich glycoprotein Gl and abolish Fusobacterium nucleatum interactions with saliva in vitro. Infect Immun 61:4434-4439.[Abstract/Free Full Text]
  • Bratthall D. (1970). Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy 21:143-152.[Medline] [Order article via Infotrieve]
  • Carlen A., Olsson J. (1995). Monoclonal antibodies against a high-molecular-weight agglutinin block adherence to experimental pellicles on hydroxyapatite and aggregation of Streptococcus mutans. J Dent Res 74:1040-1047.
  • Carlen A., Olsson J., Borjesson AC (1996). Saliva-mediated binding in vitro and prevalence in vivo of Streptococcus mutans. Arch Oral Biol 41:35-39.[Medline] [Order article via Infotrieve]
  • Carlsson P. (1988). On the epidemiology of mutans streptococci (thesis). Malmo, Sweden: Lund University.
  • Clark WB, Bammann LL, Gibbons RJ (1978). Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun 19:846-853.[Abstract/Free Full Text]
  • Emilson CG, Ciardi JE, Olsson J., Bowen WH (1989). The influence of saliva on infection of the human mouth by mutans streptococci. Arch Oral Biol 34:335-340.[CrossRef][Medline] [Order article via Infotrieve]
  • Ericson T., Pruitt K., Wedel H. (1975). The reaction of salivary substances with bacteria. J Oral Pathol 4:307-323.[CrossRef][Medline] [Order article via Infotrieve]
  • Friedman RD, Azen EA, Yu PL, Green PA, Karn RC, Merritt AD (1980). Heritable salivary proteins and dental disease. Hum Hered 30:372-375.[Medline] [Order article via Infotrieve]
  • Gahnberg L., Olsson J., Krasse B., Carlen A. (1982). Interference of salivary immunoglobulin A antibodies and other salivary fractions with adherence of Streptococcus mutans to hydroxyapatite. Infect Immun 37:401-406.[Abstract/Free Full Text]
  • Gibbons RJ (1989). Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res 68:750-760.
  • Gibbons RJ, Hay DI (1988). Human salivary acidic proline-rich proteins and statherin promote the attachment of Actinomyces viscosus LY7 to apatitic surfaces. Infect Immun 56:439-445.[Abstract/Free Full Text]
  • Gibbons RJ, Hay DI (1989). Adsorbed salivary acidic proline-rich proteins contribute to the adhesion of Streptococcus mutans JBP to apatitic surfaces. J Dent Res 68:1303-1307.
  • Gibbons RJ, van Houte J. (1975a). Dental caries. Ann Rev Med 26:121-136.[CrossRef][Medline] [Order article via Infotrieve]
  • Gibbons RJ, van Houte J. (1975b). Bacterial adherence in oral microbial ecology. Ann Rev Microbiol 29:19-44.[CrossRef][Medline] [Order article via Infotrieve]
  • Gibbons RJ, Hay DI, Schlesinger DH (1991). Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces. Infect Immun 59:2948-2954.[Abstract/Free Full Text]
  • Gillece-Castro BL, Prakobphol A., Burlingame AL, Leffler H., Fisher SJ (1991). Structure and bacterial receptor activity of a human salivary proline-rich glycoprotein. J Biol Chem 266:17358-17368.[Abstract/Free Full Text]
  • Gold OG, Jordan HV, van Houte J. (1973). A selective medium for Streptococcus mutans. Arch Oral Biol 18:1357-1364.[CrossRef][Medline] [Order article via Infotrieve]
  • Hallberg K., Holm C. Hammarstrom K-J., Kalfas S., Stromberg N. (1997). Ribotype diversity of Actinomyces with similar intraoral tropism but different GalNAcβ binding specificities. Oral Microbiol Immun (in press).
  • Hay DI, Moreno EC (1989). Statherin and the acidic proline-rich proteins. In: Human saliva: clinical chemistry and microbiology, Vol. I. Tenovuo JO, editor. Boca Raton, FL: CRC Press, Inc., pp. 131-150.
  • Hay DI, Moreno EC, Schlesinger DH (1979). Phosphoprotein-inhibitors of calcium phosphate precipitation from salivary secretions. Inorg Persp Biol Med 2:271-285.
  • Hay DI, Ahern JM, Schluckebier SK, Schlesinger DH (1994). Human salivary acidic proline-rich protein polymorphisms and biosynthesis studied by high-performance liquid chromatography. J Dent Res 73:1717-1726.
  • Hsu SD, Cisar JO, Sandberg AL, Kilian M. (1994). Adhesive properties of Viridans streptoccocal species. Microb Ecol Hlth Dis 7:125-137.
  • Jalil RA, Ashley FP, Wilson RF (1992). The relationship between 48-h dental plaque accumulation in young human adults and the concentrations of hypothiocyanite, "free" and "total" lysozyme, lactoferrin and secretory immunoglobulin A in saliva. Arch Oral Biol 37:23-28.[CrossRef][Medline] [Order article via Infotrieve]
  • Jalil RA, Ashley FP, Wilson RF, Wagaiyu EG (1993). Concentrations of thiocyanate, hypothiocyanite, "free" and "total" lysozyme, lactoferrin and secretory IgA in resting and stimulated whole saliva of children aged 12-14 years and the relationship with plaque accumulation and gingivitis. J Periodont Res 28:130-136.[CrossRef][Medline] [Order article via Infotrieve]
  • Kishimoto E., Hay DI, Kent R. ( 1990). Polymorphism of submandibular-sublingual salivary proteins which promote adhesion of Streptococcus mutans serotype-c strains to hydroxyapatite. J Dent Res 69:1741-1745.
  • Krasse B. (1966). Human streptococci and experimental caries in hamsters. Arch Oral Biol 11:429-436.[CrossRef][Medline] [Order article via Infotrieve]
  • Lamkin MS, Oppenheim FG (1993). Structural features of salivary function. Crit Rev Oral Biol Med 4:251-259.[Free Full Text]
  • Leffler H., Svanborg-Eden C. (1990). Host epithelial glycoconjugates and pathogenic bacteria. Am J Resp Cell Mol Biol 2:409-411.[Medline] [Order article via Infotrieve]
  • Lenander-Lumikari M., Tenovuo J., Emilson CG, Vilja P. (1992). Viability of Streptococus mutans and Streptococcus sobrinus in whole saliva with varying concentrations of indigenous antimicrobial agents. Caries Res 26:371-378.[Medline] [Order article via Infotrieve]
  • Lindstedt R., Larson G., Falk P., Jodal U., Leffler H., Svanborg C. (1991). The receptor repertoire defines the host range for attaching Escherichia coli strains that recognize globo-A. Infect Immun 59:1086-1092.[Abstract/Free Full Text]
  • Madapallimattam G., Bennick A. (1990). Phosphopeptides derived from human salivary acidic proline-rich proteins. Biochem J 270:297-304.[Medline] [Order article via Infotrieve]
  • Magnusson I., Ericson T., Pruitt K. (1976). Effect of salivary agglutinins on bacterial colonization of tooth surfaces. Caries Res 10:113-122.[Medline] [Order article via Infotrieve]
  • Marsh PD (1994). Microbial ecology of dental plaque and its significance in health and disease. Adv Dent Res 8:263-271.[Abstract/Free Full Text]
  • Moller AJ (1966). Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidsk 74:1-380.
  • Moreno EC, Kresak M., Hay DI (1982). Adsorption thermodynamics of acidic proline-rich human salivary proteins onto calcium apatites. J Biol Chem 257:2981-2989.[Abstract/Free Full Text]
  • Nyvad B., Kilian M. (1987). Microbiology of the early colonization of human enamel and root surfaces in vivo. Scand J Dent Res 95:369-380.[Medline] [Order article via Infotrieve]
  • Perch B., Kjems E., Ravn T. ( 1974). Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta Path Microbiol Scand 82(Sect B):357-370.
  • Perinpanayagam Her, van Wuyckhuyse BC, Ji ZS, Tabak LA (1995). Characterization of low-molecular-weight peptides in human parotid saliva. J Dent Res 74:345-350.
  • Rosan B., Appelbaum B., Golub E., Malamud D., Mandel ID (1982). Enhanced saliva-mediated bacterial aggregation and decreased bacterial adhesion in caries-resistant versus caries-susceptible individuals. Infect Immun 38:1056-1059.[Abstract/Free Full Text]
  • Rundegren J. (1986). Calcium-dependent salivary agglutinin with reactivity to various oral bacterial species. Infect Immun 53:173-178.[Abstract/Free Full Text]
  • Scannapieco FA (1994). Saliva-bacterium interactions in oral microbial ecology. Crit Rev Oral Biol Med 5:203-248.[Abstract/Free Full Text]
  • Sharon N., Lis H. (1989). Lectins as cell recognition molecules. Science 246:227-234.[Abstract/Free Full Text]
  • Silness J., Loe H. (1964). Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 22:121-135.[Medline] [Order article via Infotrieve]
  • Simonsson T., Ronstrom A., Rundegren J., Birkhed D. (1987). Rate of plaque formation-some clinical and biochemical characteristics of "heavy" and "light" plaque formers. Scand J Dent Res 95:97-103.[Medline] [Order article via Infotrieve]
  • Socransky SS, Haffajee AD (1991). Microbial mechanisms in the pathogenesis of destructive periodontal disease: a critical assessment. J Periodont Res 26:195-212.[CrossRef][Medline] [Order article via Infotrieve]
  • Stromberg N., Boren T. (1992). Actinomyces tissue specificity may depend on differences in receptor specificity for GalNAcβ-containing glycoconjugates. Infect Immun 60:3268-3277.[Abstract/Free Full Text]
  • Stromberg N., Marklund BI, Lund B., Ilver D., Hamers A., Gaastra W., et al. (1990). Host-specificity of uropathogenic Escherichia coli depends on differences in binding specificity to Galal-4Gal-containing isoreceptors. EMBO J 9:2001-2010.[Medline] [Order article via Infotrieve]
  • Stromberg N., Nyholm PG, Pascher I., Normark S. (1991). Saccharide orientation at the cell surface affects glycolipid receptor function. Proc Natl Acad Sci USA 88:9340-9344.[Abstract/Free Full Text]
  • Stromberg N., Boren T., Carlen A., Olsson J. (1992). Salivary receptors for GalNAcβ-sensitive adherence of Actinomyces spp.: Evidence for heterogeneous GalNAcβ and proline-rich protein receptor properties. Infect Immun 60:3278-3286.[Abstract/Free Full Text]
  • Stromberg N., Ahlfors S., Boren T., Bratt P., Hallberg K., Hammarstrom K-J., et al. (1996). Anti-adhesion and diagnostic strategies for oro-intestinal bacterial pathogens. In: Toward anti-adhesion therapy for microbial diseases. Kahane I, Ofek I, editors. Advances in experimental medicine and biology. Vol. 408. New York: Plenum Press, pp. 9-24.
  • Tenovuo J., Jentsch H., Soukka T., Karhuvaara L. (1992). Antimicrobial factors of saliva in relation to dental caries and salivary levels of mutans streptococci. J Biol Buccale 20:85-90.[Medline] [Order article via Infotrieve]
  • Thornton DJ, Holmes DF, Sheehan JK, Carlstedt I. (1989). Quantitation of mucus glycoproteins blotted onto nitrocellulose membranes. Anal Biochem 182:160-164.[CrossRef][Medline] [Order article via Infotrieve]
  • Wong RS, Bennick A. (1980). The primary structure of a salivary calcium-binding proline-rich phosphoprotein (protein C), a possible precursor of a related salivary protein A. J Biol Chem 255:5943-5948.[Abstract/Free Full Text]
  • Wong RS, Madapallimattam G., Bennick A. (1983). The role of glandular kallikrein in the formation of a salivary proline-rich protein A by cleavage of a single bond in salivary protein C. Biochem J 211:35-44.[Medline] [Order article via Infotrieve]
  • Xu T., Levitz SM, Diamond RD, Oppenheim FG (1991). Anticandidal activity of major human salivary histatins. Infect Immun 59:2549-2554.[Abstract/Free Full Text]
  • Yu PL, Bixler D., Goodman PA, Azen EA, Karn RC ( 1986). Human parotid proline-rich proteins: Correlation of genetic polymorphisms to dental caries. Genet Epidemiol 3:147-152.[Medline] [Order article via Infotrieve]
  • Zylber LJ, Jordan HV (1982). Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque. J Clin Microbiol 15:253-259.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 77, No. 1, 81-90 (1998)
DOI: 10.1177/00220345980770011301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M.-R. Sarrias, S. Rosello, F. Sanchez-Barbero, J. M. Sierra, J. Vila, J. Yelamos, J. Vives, C. Casals, and F. Lozano
A Role for Human SP{alpha} as a Pattern Recognition Receptor
J. Biol. Chem., October 21, 2005; 280(42): 35391 - 35398.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. J. Bikker, A. J. M. Ligtenberg, C. End, M. Renner, S. Blaich, S. Lyer, R. Wittig, W. van't Hof, E. C. I. Veerman, K. Nazmi, et al.
Bacteria Binding by DMBT1/SAG/gp-340 Is Confined to the VEVLXXXXW Motif in Its Scavenger Receptor Cysteine-rich Domains
J. Biol. Chem., November 12, 2004; 279(46): 47699 - 47703.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Oho, F. J. Bikker, A. V. Nieuw Amerongen, and J. Groenink
A Peptide Domain of Bovine Milk Lactoferrin Inhibits the Interaction between Streptococcal Surface Protein Antigen and a Salivary Agglutinin Peptide Domain
Infect. Immun., October 1, 2004; 72(10): 6181 - 6184.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
T. Li, M. K. Khah, S. Slavnic, I. Johansson, and N. Stromberg
Different Type 1 Fimbrial Genes and Tropisms of Commensal and Potentially Pathogenic Actinomyces spp. with Different Salivary Acidic Proline-Rich Protein and Statherin Ligand Specificities
Infect. Immun., December 1, 2001; 69(12): 7224 - 7233.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
R. L. Gregory
Modified Immunogenicity of a Mucosally Administered Antigen
Clin. Vaccine Immunol., May 1, 2001; 8(3): 540 - 544.
[Abstract] [Full Text] [PDF]

Home page
 ADRHome page
J.D. Rudney
Saliva and Dental Plaque
Advances in Dental Research, December 1, 2000; 14(1): 29 - 39.
[Abstract] [PDF]

Home page
 Infect. Immun.Home page
T. Li, P. Bratt, A. P. Jonsson, M. Ryberg, I. Johansson, W. J. Griffiths, T. Bergman, and N. Stromberg
Possible Release of an ArgGlyArgProGln Pentapeptide with Innate Immunity Properties from Acidic Proline-Rich Proteins by Proteolytic Activity in Commensal Streptococcus and Actinomyces Species
Infect. Immun., September 1, 2000; 68(9): 5425 - 5429.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
P. Bratt, D. Boren, T. Boren, and N. Stromberg
Secretory Immunoglobulin A Heavy Chain Presents Gal{beta}1-3GalNAc Binding Structures for Actinomyces naeslundii genospecies 1
Journal of Dental Research, June 1, 1999; 78(6): 1238 - 1244.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. Prakobphol, F. Xu, V. M. Hoang, T. Larsson, J. Bergstrom, I. Johansson, L. Frangsmyr, U. Holmskov, H. Leffler, C. Nilsson, et al.
Salivary Agglutinin, Which Binds Streptococcus mutans and Helicobacter pylori, Is the Lung Scavenger Receptor Cysteine-rich Protein gp-340
J. Biol. Chem., December 15, 2000; 275(51): 39860 - 39866.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Carlén, A.
Right arrow Articles by Strömberg, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carlén, A.
Right arrow Articles by Strömberg, N.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?