Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gemmell, E.
Right arrow Articles by Seymour, G.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gemmell, E.
Right arrow Articles by Seymour, G.J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Cytokine Profiles of Cells Extracted from Humans with Periodontal Diseases

E. Gemmell

Immunopathology Laboratory, Oral Biology and Pathology, Department of Dentistry, The University of Queensland, Queensland 4072, Australia

G.J. Seymour

Immunopathology Laboratory, Oral Biology and Pathology, Department of Dentistry, The University of Queensland, Queensland 4072, Australia

FACS analysis was used to determine the percent interferon (IFN)-gamma-, interleukin (IL)-4-, IL-10-, and CD30-positive T-cells extracted from adult periodontitis (AP) and healthy/gingivitis (H/G) subjects. Additionally, the percentages of IL-1β-, IL-10- and IL-12-producing B-cells and macrophages were ascertained. The percent IL-10+ CD8 cells extracted from AP lesions was decreased compared with H/G tissues (p = 0.033), and the percent CD30+ CD4 (p = 0.001) and CD30+ CD8 (p = 0.028) cells was higher in AP than in H/G tissues. The percentages of IL-1β+ macrophages (p = 0.003) and IL-12+ B-cells (p = 0.034) were both higher in AP lesions than in H/G tissues. The specific effect of Porphyromonas gingivalis on the cytokine profiles of peripheral blood mononuclear cells isolated from P. gingival is-infected AP and H/G patients was also determined. While there were no significant differences in the percent cytokine-positive T-cells after stimulation with P. gingival is outer membrane antigens (OM) for 6 days compared with cells incubated in medium only, the percent CD30+ CD4 cells increased significantly (p = 0.047 and p = 0.063 for AP and H/G groups, respectively). There was also an increase in the percent IL-1β+ B-cells from AP patients (p = 0.029), and the percent IL-12+ monocytes from AP and H/G subjects was higher than the percent IL-12+ B-cells, both after stimulation with P. gingivalis OM (p = 0.005 for the AP group and p = 0.058 and therefore not quite significant for the H/G group) and when incubated in medium alone (p = 0.016 and p = 0.015 for AP and H/G groups, respectively). This study has shown that IL-10+ CD8 cells may be significant in gingival lesions, and that CD30+ T-cells indicative of Th2 or Th0 cells may play a role in progressive periodontal disease. This study has also shown that B-cells produce IL-1 in the gingival lesion and that P. gingivalis may be significant in the induction of B-cell-induced IL-1.

Key Words: cytokines • T-lymphocytes • periodontal diseases • B-lymphocytes • macrophages.

REFERENCES

  • Andersson U., Andersson J., Lindfors A., Wagner K., Moller G., Heusser CH (1990). Simultaneous production of interleukin 2, interleukin 4 and interferon-{gamma} by activated human blood lymphocytes. Eur J Immunol 20:1591-1596.[Medline] [Order article via Infotrieve]
  • Bird PS ( 1992). The production and characterization of monoclonal antibodies to Fusobacterium nucleatum FDC 263 and Porphyromonas gingivalis FDC 381, and the nature of the epitopes (PhD thesis). Queensland, Australia: The University of Queensland.
  • Bird PS, Seymour GJ (1987). Production of monoclonal antibodies that recognize specific and cross-reactive antigens of Fusobacterium nucleatum. Infect Immun 55:771-777.[Abstract/Free Full Text]
  • Birkedal-Hansen H. (1993). Roles of cytokines and inflammatory mediators in tissue destruction. J Periodont Res 28:500-510.[CrossRef][Medline] [Order article via Infotrieve]
  • Chang TL, Shea CM, Urioste S., Thompson RC, Boom WH, Abbas AK (1990). Heterogeneity of helper/inducer T lymphocytes. 3. Responses of IL-2- and IL-4-producing (Thl and Th2) clones to antigens presented by different accessory cells. J Immunol 145:2803-2808.[Abstract]
  • Cole KL, Seymour GJ, Powell RN (1987). Phenotypic and functional analysis of T cells extracted from chronically inflamed human periodontal tissues. J Periodontol 58:569-573.[Medline] [Order article via Infotrieve]
  • Daly CG, Cripps AW, Clancy RL (1983). Lymphocytes from chronically inflamed human gingiva. 2. Immunoglobulin production in vitro. J Periodont Res 8:132-138.
  • Del Prete G., Maggi E., Pizzolo G., Romagnani S. (1995). CD30, Th2 cytokines and HIV infection: a complex and fascinating link. Immunol Today 16:76-80.[CrossRef][Medline] [Order article via Infotrieve]
  • Deslauriers M., ni Eidhin D., Lamonde L., Mouton C. (1990). SDS-PAGE analysis of protein and lipopolysaccharide of extracellular vesicules and sarkosyl-insoluble membranes from Bacteroides gingivalis. Oral Microbiol Immunol 5:1-7.[Medline] [Order article via Infotrieve]
  • de Waal Malefyt R., Yssel H., Roncarolo MG, Spits H., de Vries JE (1992). Interleukinl-10. Curr Opin Immunol 4:314-320.[CrossRef][Medline] [Order article via Infotrieve]
  • de Waal Malefyt R., Yssel H., de Vries JE (1993). Direct effects of IL-10 on subsets of human CD4+ T cell clones and resting T cells. Specific inhibition of IL-2 production and proliferation. J Immunol 150(11):4754-4765.[Abstract]
  • Durkop H., Latza U., Hummel M., Eitelbach F., Seed B., Stein H. (1992). Molecular cloning and expression of a new member of the nerve growth factor receptor family that is characteristic for Hodgkin's disease. Cell 68:421-427.[CrossRef][Medline] [Order article via Infotrieve]
  • Ebersole JL, Taubman MA (1994). The protective nature of host responses in periodontal diseases. Periodontol 2000 5:112-141.
  • Evans RI, Mikulecky M., Seymour GJ (1989). Effect of initial treatment of chronic inflammatory periodontal disease in adults on spontaneous peripheral blood lymphocyte proliferation. J Clin Periodontol 16:271-277.[Medline] [Order article via Infotrieve]
  • Fujihashi K., Yamamoto M., Hiroi T., Bamberg TV, McGhee JR, Kiyono H. (1996). Selected Th1 and Type 2 cytokine mRNA expression by CD4(+) T cells isolated from inflamed human gingival tissues. Clin Exp Immunol 103:422-428.[CrossRef][Medline] [Order article via Infotrieve]
  • Gemmell E., Seymour GJ (1991). Phenotypic analysis of B-cells extracted from human periodontal disease tissue. Oral Microbiol Immunol 6:356-362.[Medline] [Order article via Infotrieve]
  • Gemmell E., Seymour GJ (1994). Modulation of immune responses to periodontal bacteria. Curr Opin Periodont (2nd ed):28-38.
  • Gemmell E., Kjeldsen M., Yamazaki K., Nakajima T., Aldred MJ, Seymour GJ (1995a). Cytokine profiles of Porphyromonas gingivalis-reactive T lymphocyte lines and clones derived from P. gingivalis-infected subjects. Oral Diseases 1:139-146.[Medline] [Order article via Infotrieve]
  • Gemmell E., Polak B., Reinhardt RA, Eccleston J., Seymour GJ (1995b). Antibody responses of Porphyromonas gingivalis infected gingivitis and periodontitis subjects. Oral Diseases 1:63-69.[Medline] [Order article via Infotrieve]
  • Gemmell E., Woodford V., Seymour GJ (1996). Characterization of T lymphocyte clones derived from Porphyromonas gingivalis infected subjects. J Periodont Res 31:47-56.[Medline] [Order article via Infotrieve]
  • Genco RJ, Slots J. (1984). Host responses in periodontal diseases. J Dent Res 63:441-451.
  • Germann T., Gately MK, Schoenhaut DS, Lohoff M., Mattner F., Fischer S., et al. (1993). Interleukin-12/T cell stimulating factor, a cytokine with multiple effects of T helper type 1 (Thl) but not on Th2 cells. Eur J Immunol 23:1762-1770.[Medline] [Order article via Infotrieve]
  • Honig J., Rordorf-Adam C., Siegmund C., Wiedemann W., Erard F. (1989). Increased interleukin-1 beta (IL-1β) concentration in gingival tissue from periodontitis patients. J Periodont Res 24:362-367.[CrossRef][Medline] [Order article via Infotrieve]
  • Jeannin P., Delneste Y., Life P., Gauchat JF, Kaiserlian D., Bonnefoy JY (1995). Interleukin-12 increases interleukin-4 production by established human Th0 and Th2-like T cell clones. Eur J Immunol 25:2247-2252.[Medline] [Order article via Infotrieve]
  • Kelso A. (1995). Th1 and Th2 subsets: paradigms lost? Immunol Today 16:374-379.[CrossRef][Medline] [Order article via Infotrieve]
  • Lamont AG, Adorini L. (1996). IL-12: a key cytokine in immune regulation. Immunol Today 17:214-217.[CrossRef][Medline] [Order article via Infotrieve]
  • Mahanonda R., Seymour GJ, Powell LW, Good MF, Halliday JW (1989). Limit dilution analysis of peripheral blood T lymphocytes specific to periodontopathic bacteria. Clin Exp Immunol 75:245-251.[Medline] [Order article via Infotrieve]
  • Manhart SS, Reinhardt RA, Payne JB, Seymour GJ, Gemmell E., Dyer JK, et al. (1994). Gingival cell IL-2 and IL-4 in early-onset periodontitis. J Periodontol 65:807-813.[Medline] [Order article via Infotrieve]
  • Matsuki Y., Yamamoto T., Hara K. ( 1993). Localization of interleukin-1 (IL-1) mRNA-expressing macrophages in human inflamed gingiva and IL-1 activity in gingival crevicular fluid. J Periodont Res 28:35-42.[CrossRef][Medline] [Order article via Infotrieve]
  • Mayrand D., Holt SC ( 1988). Biology of asaccharolytic Bacteroides species. Microbiol Rev 2:134-152.
  • Modlin RL, Nutman TB (1993). Type 2 cytokines and negative immune regulation in human infections. Curr Opin Immunol 5:511-517.[CrossRef][Medline] [Order article via Infotrieve]
  • Morris AG (1988). Interferons. Immunol l(Suppl):43-45.
  • Mosmann TR, Coffman RL (1989). TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Ann Rev Immunol 7:145-173.[CrossRef][Medline] [Order article via Infotrieve]
  • Mosmann TR, Sad S. (1996). The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 17:138-146.[CrossRef][Medline] [Order article via Infotrieve]
  • Ni Eidhin D., Mouton C. (1993). A rapid method for preparation of rough and smooth lipopolysaccharide from Bacteroides, Porphyromonas and Prevotella. FEMS Microbiol Lett 110:133-138.[CrossRef][Medline] [Order article via Infotrieve]
  • O'Garra A. (1989). Peptide regulatory factors. Interleukins and the immune system 2. The Lancet 1:1003-1005.
  • Okuda K., Takazoe I. (1988). The role of Bacteroides gingivalis in periodontal disease. Adv Dent Res 2:260-268.[Abstract/Free Full Text]
  • Orange JS, Wolf SF, Biron CA (1994). Effects of IL-12 on the response and susceptibility to experimental viral infections. J Immunol 152:1253-1264.[Abstract]
  • Page RC ( 1991). The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodont Res 26:230-242.[CrossRef][Medline] [Order article via Infotrieve]
  • Prabhu A., Michalowicz BS, Mathur A. (1996). Detection of local and systemic cytokines in adult periodontitis. J Periodontol 67:515-522.[Medline] [Order article via Infotrieve]
  • Roodman GD (1992). Interleukin-6: An osteotropic factor? J Bone Miner Res 7:475-478.[Medline] [Order article via Infotrieve]
  • Rousset F., Garcia E., Defrance T., Peronne C., Vezzio N., Hsu DH, et al. (1992). Interleukin 10 is a potent growth and differentiation factor for activated human B lymphocytes. Proc Natl Acad Sci USA 89:1890-1893.[Abstract/Free Full Text]
  • Sander B., Andersson J., Andersson U. (1991). Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev 119:65-93.[CrossRef][Medline] [Order article via Infotrieve]
  • Schwab U., Stein H., Gerdes J., Lemke H., Kirchner H., Schaadt M., et al. (1982). Production of a monoclonal antibody specific for Hodgkin and Sternberg-Reed cells of Hodgkin's disease and a subset of normal lymphoid cells. Nature 299(5878):65-67.[CrossRef][Medline] [Order article via Infotrieve]
  • Scott P. (1993). Selective differentiation of CD4+ T helper cell subsets. Curr Opin Immunol 5:391-397.[CrossRef][Medline] [Order article via Infotrieve]
  • Seymour GJ (1987). Possible mechanisms involved in the immunoregulation of chronic inflammatory periodontal disease. J Dent Res 66:2-9.
  • Seymour GJ (1991). Importance of the host response in the periodontium. J Clin Periodontol 18:421-426.[CrossRef][Medline] [Order article via Infotrieve]
  • Seymour GJ, Greenspan JS (1979). The phenotypic characterization of lymphocyte subpopulations in established human periodontal disease. J Periodont Res 14:39-46.[CrossRef][Medline] [Order article via Infotrieve]
  • Seymour GJ, Powell RN, Aitken JF (1983). Experimental gingivitis in humans: a clinical and histologic investigation. J Periodontol 54:522-528.[Medline] [Order article via Infotrieve]
  • Seymour GJ, Gemmell E., Walsh LJ, Powell RN (1988). Immunohistological analysis of experimental gingivitis in humans. Clin Exp Immunol 71:132-137.[Medline] [Order article via Infotrieve]
  • Smith CA, Davis T., Anderson D., Solam L., Beckmann MP, Jerzy R., et al. (1990). A receptor for tumour necrosis factor defines an unusual family of cellular and viral proteins. Science 248(4958):1019-1023.[Abstract/Free Full Text]
  • Stashenko P., Dewhirst FE, Peros WJ, Kent RL, Ago JM (1987). Synergistic interactions between interleukin 1, tumor necrosis factor, and lymphotoxin in bone resorption. J Immunol 138:1464-1468.[Abstract]
  • Stein H., Mason DY, Gerdes J., O'Connor N., Wainscoat J., Pallesen G., et al. (1985). The expression of the Hodgkin's disease associated antigen Ki-1 in reactive and neoplastic lymphoid tissue: evidence that Reed-Sternberg cells and histiocytic malignancies are derived from activated lymphoid cells. Blood 66:848-858.[Abstract/Free Full Text]
  • Street NE, Mosmann TR (1991). Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J 5:171-177.[Abstract]
  • Takeichi 0, Taubman MA, Haber J., Smith DJ, Moro I. ( 1994). Cytokine profiles of CD4 and CD8 T cells isolated from adult periodontitis gingivae (abstract). J Dent Res 73:205.
  • Taubman MA, Stoufi ED, Ebersole JL, Smith DJ (1984). Phenotypic studies of cells from periodontal disease tissues. J Periodont Res 19:587-590.[CrossRef][Medline] [Order article via Infotrieve]
  • Tew J., Engel D., Mangan D. (1989). Polyclonal B-cell activation in periodontitis. J Periodont Res 24:225-241.[CrossRef][Medline] [Order article via Infotrieve]
  • Trinchieri G. (1993). Interleukin-12 and its role in the generation of TH1 cells. Immunol Today 14:335-338.[CrossRef][Medline] [Order article via Infotrieve]
  • Tsicopoulos A., Hamid Q., Varney V., Ying S., Moqbel R., Durham SR, et al. (1992). Preferential messenger RNA expression of Thl-type cells (IFN-gamma+, IL-2+) in classical delayed-type (tuberculin) hypersensitivity reactions in human skin. J Immunol 148:2058-2061.[Abstract]
  • Watanabe H., Marsh PD, Ivanyi L. (1989). Detection of immunodominant antigens of periodontopathic bacteria in human periodontal disease. Oral Microbiol Immunol 4:159-164.[Medline] [Order article via Infotrieve]
  • Yamamoto M., Fujihashi K., Hiroi T., McGhee JR, Van Dyke TE, Kiyono H. (1997). Molecular and cellular mechanisms for periodontal diseases: Role of Th1 and Th2 type cytokines in induction of mucosal inflammation. J Periodont Res 32:115-119.[CrossRef][Medline] [Order article via Infotrieve]
  • Yamamura M., Wang XH, Ohmen JD, Uyemura K., Rea TH, Bloom BR, et al. (1992). Cytokine patterns of immunologically mediated tissue damage. J Immunol 149:1470-1475.[Abstract]

Journal of Dental Research, Vol. 77, No. 1, 16-26 (1998)
DOI: 10.1177/00220345980770010101
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Journal of the American Dental AssociationHome page
C. S. Miller, C. P. King Jr., M. C. Langub, R. J. Kryscio, and M. V. Thomas
Salivary biomarkers of existing periodontal disease: A cross-sectional study
J Am Dent Assoc, March 1, 2006; 137(3): 322 - 329.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
P. Ford, E. Gemmell, P. Walker, M. West, M. Cullinan, and G. Seymour
Characterization of Heat Shock Protein-Specific T Cells in Atherosclerosis
Clin. Vaccine Immunol., February 1, 2005; 12(2): 259 - 267.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
Y.-T. A. Teng
THE ROLE OF ACQUIRED IMMUNITY AND PERIODONTAL DISEASE PROGRESSION
Critical Reviews in Oral Biology & Medicine, July 1, 2003; 14(4): 237 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
Y.-T. A. Teng
Mixed Periodontal Th1-Th2 Cytokine Profile in Actinobacillus actinomycetemcomitans-Specific Osteoprotegerin Ligand (or RANK-L)- Mediated Alveolar Bone Destruction In Vivo
Infect. Immun., September 1, 2002; 70(9): 5269 - 5273.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
R. Tamai, T. Sakuta, K. Matsushita, M. Torii, O. Takeuchi, S. Akira, S. Akashi, T. Espevik, S. Sugawara, and H. Takada
Human Gingival CD14+ Fibroblasts Primed with Gamma Interferon Increase Production of Interleukin-8 in Response to Lipopolysaccharide through Up-Regulation of Membrane CD14 and MyD88 mRNA Expression
Infect. Immun., March 1, 2002; 70(3): 1272 - 1278.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
E. Gemmell, K. Yamazaki, and G.J. Seymour
DESTRUCTIVE PERIODONTITIS LESIONS ARE DETERMINED BY THE NATURE OF THE LYMPHOCYTIC RESPONSE
Critical Reviews in Oral Biology & Medicine, January 1, 2002; 13(1): 17 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. L. W. Yun, A. A. Decarlo, C. Collyer, and N. Hunter
Hydrolysis of Interleukin-12 by Porphyromonas gingivalis Major Cysteine Proteinases May Affect Local Gamma Interferon Accumulation and the Th1 or Th2 T-Cell Phenotype in Periodontitis
Infect. Immun., September 1, 2001; 69(9): 5650 - 5660.
[Abstract] [Full Text] [PDF]

Home page
 CROBMHome page
M.A. Taubman and T. Kawai
Involvement of T-Lymphocytes in Periodontal Disease and in Direct and Indirect Induction of Bone Resorption
Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(2): 125 - 135.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. L. W. Yun, A. A. DeCarlo, and N. Hunter
Modulation of Major Histocompatibility Complex Protein Expression by Human Gamma Interferon Mediated by Cysteine Proteinase-Adhesin Polyproteins of Porphyromonas gingivalis
Infect. Immun., June 1, 1999; 67(6): 2986 - 2995.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Christgau, R. G. Caffesse, J. R. Newland, G. Schmalz, and R. N. D'Souza
Characterization of Immunocompetent Cells in the Diseased Canine Periodontium
J. Histochem. Cytochem., December 1, 1998; 46(12): 1443 - 1454.
[Abstract] [Full Text]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Gemmell, E.
Right arrow Articles by Seymour, G.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gemmell, E.
Right arrow Articles by Seymour, G.J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?