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Journal of Dental Research
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HRTEM Study of Biological Crystal Growth Mechanisms in the Vicinity of Implanted Synthetic Hydroxyapatite Crystals

J. Hemmerlé

Institut National de la Sante et de la Recherche Medicale, Unite 424, Federation de Recherches Odontologie, 11, rue Humann, 67085 Strasbourg France

F.J.G. Cuisinier

Institut National de la Sante et de la Recherche Medicale, Unite 424, Federation de Recherches Odontologie, 11, rue Humann, 67085 Strasbourg France

P. Schultz

Institut de Genetique et de Biologie Moleculaire et Cellulaire, 1 rue Laurent Fries, 67404 Illkirch, France

J.-C. Voegel

Institut National de la Sante et de la Recherche Medicale, Unite 424, Federation de Recherches Odontologie, 11, rue Humann, 67085 Strasbourg France

Calcium phosphates are widely used as biomaterials. Ultrastructural assessments are of the utmost importance in our understanding of interfacial phenomena. The aim of this study was to learn more about the newly formed crystal growth mechanisms. The interfaces between implanted synthetic hydroxyapatite crystals (HAS) and newly formed crystallites were thoroughly examined on a molecular level. The bone-grafting material (HAS) was implanted into two adult patients, and small biopsies were recovered 6 months after implantation. The raw biomaterial was analyzed by x-ray diffraction and high-resolution transmission electron microscopy (HRTEM). Six months after their implantation, the HAS aggregates were surrounded by a mineralized bone matrix. Tiny crystallites also filled the spaces between the HAS crystals within the aggregates. These newly formed crystallites growing at the surfaces of the implanted HAS crystals appeared to be apatitic. The crystallographic investigations of the nucleation and growing mechanisms of the newly formed crystallites were performed by HRTEM in association with computer simulation and mathematical processing of digitized images. A relationship was noted between the orientation axes of crystallites growing nearby and the zone axes of the implanted HAS, thus strongly suggesting a guiding or substratum role of the HAS particles.

Key Words: hydroxyapatite • bone-graftin • , high-resolution TEM • hydroxyapatite/bone interface • crystal growth. Received November 6, 1995; Accepted September 13,1996

REFERENCES

  • Bagambisa FB, Joos U., Schilli W. (1993). Mechanisms and structure of the bond between bone and hydroxyapatite ceramics. J Biomed Mater Res 27:1047-1055.[CrossRef][Medline] [Order article via Infotrieve]
  • Bay BK, Martin RB, Sharkey NA, Chapman MW (1993). Repair of large cortical defects with block coralline hydroxyapatite. Bone 14:225-230.
  • Daculsi G., LeGeros RZ, Nery E., Lynch K., Kerebel B. (1989). Transformation of biphasic calcium phosphate ceramics in vivo: Ultrastructural and physicochemical characterization. J Biomed Mater Res 23:883-894.[Medline] [Order article via Infotrieve]
  • Damen JJ, Ten Cate JM, Ellingsen JE (1991). Induction of calcium phosphate precipitation by titanium dioxide. J Dent Res 70:1346-1349.
  • De Bruijn JD, van Blitterswijk CA, Davies JE (1995). Initial bone matrix formation at the hydroxyapatite interface in vivo. J Biomed Mater Res 29:89-99.[Medline] [Order article via Infotrieve]
  • De Lange GL, De Putter C., De Wijs FL (1990). Histological and ultrastructural appearance of the hydroxyapatite-bone interface. J Biomed Mater Res 24:829-845.[Medline] [Order article via Infotrieve]
  • Ducheyne P., Radin S., King L. ( 1993). The effect of calcium phosphate ceramic composition and structure on in vitro behavior. 1. Dissolution. J Biomed Mater Res 27:25-34.
  • Frank RM, Klewansky P., Hemmerle J., Tenenbaum H. (1991a). Ultrastructural demonstration of the importance of crystal size of bioceramic powders implanted into human periodontal lesions. J Clin Periodontol 18:669-680.[Medline] [Order article via Infotrieve]
  • Frank RM, Wiedemann P., Hemmerle J., Freymann M. (1991b). Pulp capping with synthetic hydroxyapatite in human premolars. J Appl Biomater 2:243-250.
  • Garcia R., Doremus RH (1992). Electron microscopy of the bone-hydroxyapatite interface from a human dental implant. J Mater Sci Mater Med 3:154-156.
  • Hayashi K., Matsuguchi N., Uenoyama K., Kanemaru T., Sugioka Y. (1989). Evaluation of metal implants coated with several types of ceramics as biomaterials. J Biomed Mater Res 23:1247-1259.[Medline] [Order article via Infotrieve]
  • Inadome T., Hayashi K., Nakashima Y., Tsumura H., Sugioka Y. (1995). Comparison of bone-implant interface shear strength of hydroxyapatite-coated and alumina-coated metal implants. J Biomed Mater Res 29:19-24.[Medline] [Order article via Infotrieve]
  • Ivarsson BA, Hegg PO, Lundstrom KI, Jonsson U. (1985). Adsorption of proteins on metal surfaces sudied by ellipsometric and capacitance measurements. Coll and Surf 13:169-192.
  • Jansen JA, van der Waerden JP, Wolke Jgc (1993). Histologic investigation of the biologic behavior of different hydroxyapatite plasma-sprayed coatings in rabbits. J Biomed Mater Res 27:603-610.[Medline] [Order article via Infotrieve]
  • Kasemo B., Lausmaa J. (1991). The biomaterial-tissue interface and its analogues in surface science and technology. In: The bone-biomaterial interface. Davies JE, editor. Toronto: University of Toronto Press, pp. 19-29.
  • Kay MI, Young RA, Posner AS (1964). Crystal structure of hydroxyapatite. Nature 204:1050-1052.[CrossRef][Medline] [Order article via Infotrieve]
  • Kotani S., Fujita Y., Kitsugi T., Nakamura T., Yamamuro T., Ohtsuki C., et al. (1991). Bone bonding mechanism of β-tricalcium phosphate. J Biomed Mater Res 25:1303-1315.[CrossRef][Medline] [Order article via Infotrieve]
  • Mura MJ, Behr S., Bres EF, Voegel JC (1990). Dynamic processes at the liquid/solid interface in the albumin/apatite system. Clin Mater 5:285-295.
  • Nelson DG, Barry JC (1989). High resolution electron microscopy of nonstoichiometric apatite crystals. Anat Rec 224:265-276.[Medline] [Order article via Infotrieve]
  • Ogilvie A., Frank RM, Benque EP, Gineste M., Heughebaert M., Hemmerle J. (1987). The biocompatibility of hydroxyapatite implanted in the human periodontium. J Periodont Res 22:270-283.[Medline] [Order article via Infotrieve]
  • Ogiso M., Tabata T., Ichijo T., Borgese D. (1992). Bone calcification on the hydroxyapatite dental implant and the bone-hydroxyapatite interface. J Long-Term Effects of Med Impl 2:137-148.
  • Oguchi H., Ishikawa K., Mizoue K., Seto K., Eguchi G. (1995). Long-term histological evaluation of hydroxyapatite ceramics in human. Biomaterials 16:33-38.
  • Orly I., Gregoire M., Menanteau J., Heughebaert M., Kerebel B. (1989). Chemical changes in hydroxyapatite biomaterial under in vivo and in vitro biological conditions. Calcif Tissue Int 45:20-26.[Medline] [Order article via Infotrieve]
  • Radin SR, Ducheyne P. (1993). The effect of calcium phosphate ceramic composition and structure on in vitro behavior. II. Precipitation. J Biomed Mater Res 27:35-45.[CrossRef][Medline] [Order article via Infotrieve]
  • Stadelmann P. (1987). EMSA software package for electron diffraction analysis and HREM image simulation in material science. Ultramicroscopy 21:131-146.[CrossRef]
  • Trombe JC (1972). Contribution à l'étude de la decomposition et de la reactivite de certaines apatites hydroxylees ou fluorees alcalino-terreuse (PhD thesis). University of Toulouse, France.
  • van Heel M., Keegstra W. (1981). Imagic: a fast, flexible and friendly image analysis sofware system. Ultramicroscopy 7:113-130.[CrossRef]
  • Yamaguchi K., Hirano T., Yoshida G., Iwasaki K. (1995). Degradation-resistant character of synthetic hydroxyapatite blocks filled in bone defects. Biomaterials 16:983-985.
  • Yoshinari M., Ohtsuka Y., Derand T. (1994). Thin hydroxyapatite coating produced by the ion beam dynamic mixing method. Biomaterials 15:529-535.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 76, No. 2, 682-687 (1997)
DOI: 10.1177/00220345970760020901
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This Article
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What's this?