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An FT-Raman Spectroscopic Investigation of Dentin and Collagen Surfaces Modified by 2-Hydroxyethylmethacrylate

Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, Quebec, Canada H3A 2K6

I. Stangel

Faculty of Dentistry, McGill University, 740 Docteur Penfield, Montreal, Quebec, Canada H3A 1A4

I.S. Butler

Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, Quebec, Canada H3A 2K6

D.F.R. Gilson

Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, Quebec, Canada H3A 2K6

Although 2-hydroxyethylmethacrylate (HEMA) is commonly used for adhesive bonding to dentin, its role in promoting adhesion is not completely understood. Here, we use FT-Raman spectroscopy to elucidate further the nature of the interaction of HEMA with dentin. Ground dentin was exposed to 2.5% (w/w) nitric acid, washed, dried in air, and treated with HEMA. The samples were then sequentially washed with distilled water, with FT-Raman spectra being obtained after different wash times. Hydroxyapatite and bovine type I collagen were similarly treated with HEMA except for the acid exposure. The FT-Raman spectra of these samples were also recorded. The spectra of HEMA-treated, water-washed dentin and collagen revealed the following changes: (1) The band intensities of HEMA absorbed on dentin and collagen decreased with increasing wash times; (2) the v(C=O) and v(CCO) modes of HEMA at 1718 and 607 cm ^-1, respectively, either disappeared or decreased after extensive washing; (3) the v(C=C) (1640 cm^-1) and (=CH₂) (1403 cm ^-1) bands exhibited minor variations in band position and relative intensity. These results demonstrate that HEMA interacts with dentin both physically and chemically. The chemical interaction can be interpreted by either hydrogen bonding or the formation of a new bond to the ester group of HEMA.

Key Words: adhesion • dentin • FT-Raman • spectroscopy • HEMA.

REFERENCES

• Bertoluzza A., Monti P., Garcia-Ramos JV, Simoni R., Caramazza R., Calzavara A. (1986). Applications of Raman spectroscopy to the ophthalmological field: Raman spectra of soft contact lenses made of poly-2-hydroxyethylmethacrylate (HEMA). J Mol Struct 143:469-472.
• Bellamy LJ (1975). The infrared spectra of complex molecules. New York: John Wiley & Sons.
• Blakeslee KC, Condrate RA (1971). Vibrational spectra of hydrothermally prepared hydroxyapatites. J Am Ceram Soc 54:560-563.
• Di Renzo M., Ellis TH, Domingue A., Bertrand L., Sacher E., Stangel I. (1994). Chemical reactions between dentin and bonding agents. J Adhesion 74:115-121.
• Di Renzo M., Ellis T., Sacher E., Stangel I. (1995). Adhesion to mineralized tissue: Bonding to human dentin. Progr Surf Sci 50:407-418.
• Dollish FR, Fateley WG, Bentley FF (1974). Amids, amino acids, peptides, and proteins. In: Characteristic Raman frequencies of organic compounds. Fately WG, Bentley FF, editors. New York: John Wiley & Sons, p. 123.
• Eick JD, Robinson SJ, Chappell RP, Cobb CM, Spencer P. (1993). The dentinal surface: Its influence on dentinal adhesion. Part III. Quintessence Int 24:571-582.[Medline] [Order article via Infotrieve]
• Eliades G., Palaghias G., Vougiouklakis G. (1990). Surface reactions of adhesives on dentin. Dent Mater 6:208-216.[Medline] [Order article via Infotrieve]
• Erickson RL (1989). Mechanism and clinical implication of bond formation for two dentin bonding agents. Am J Dent 2:117-123.
• Frushour BG, Koenig JL (1975). Raman scattering of collagen, gelatin and elastin. Biopolymers 14:379-391.[CrossRef][Medline] [Order article via Infotrieve]
• Fusayama T., Nakamura M., Kurosaki N., Iwaku M. (1979). Non-pressure adhesion of a new adhesive restorative resin. J Dent Res 58:1364-1370.
• Goheen SC, Lis LJ, Kauffman JW (1978). Raman spectroscopy of intact feline comeal collagen. Biochim Biophys Acta 536:197-204.[Medline] [Order article via Infotrieve]
• Nakabayashi N., Takarada K. (1992). Effect of HEMA on bonding to dentin. Dent Mater 8:125-130.[Medline] [Order article via Infotrieve]
• Nakabayashi N., Kojima K., Masuhara E. (1982). The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 16:265-273.[CrossRef][Medline] [Order article via Infotrieve]
• O'Shea DC, Bartlett ML, Young RA (1974). Compositional analysis of apatites with laser-Raman spectroscopy: (OH, F, Cl) apatites. Arch Oral Biol 19:995-1006.[Medline] [Order article via Infotrieve]
• Pashley DH, Ciucchi B., Sano H., Homer JA (1993). Permeability of dentin to adhesive agents. Quintessence Int 24:618-631.[Medline] [Order article via Infotrieve]
• Spencer P., Byerley TJ, Eick JD, Witt JD ( 1992). Chemical characterization of the dentin/adhesive interface by Fourier transform infrared photoacoustic spectroscopy. Dent Mater 8:10-15.[Medline] [Order article via Infotrieve]
• Stangel I., Ostro E., Domingue A., Sacher E., Bertrand L. (1991). Photoacoustic Fourier transform IR spectroscopic study of polymer-dentin interaction. In: Polymer-solid interfaces. Pireaux JJ, Bertrand P, Bredas J.L, editors. Bristol and Philadelphia: Institute of Physics Publishing, pp. 157-167.
• Stangel I., Sacher E., Young C., Hanley S. (1994). The effect of conditioning on adhesion to human dentin. J Adhesion 47:133-149.
• Sugizaki J. (1991). The effect of the various primers on the dentin adhesion of resin composites-SEM and TEM observations of the resin-impregnated layer and adhesion promoting effect of primers. Jpn J Conserv Dent 34:228-265.
• Suzuki M., Kato H., Wakumoto S. (1991). Vibrational analysis by Raman spectroscopy of the interface between dental adhesive resin and dentin. J Dent Res 70:1092-1097.
• Van Meerbeek B., Inokoshi S., Braem M., Lambrechts P., Vanherle G. (1992). Morphological aspects of the resin-dentin interdiffusion zone with different dentin adhesive systems. J Dent Res 71:1530-1540.
• Van Meerbeek B., Dhem A., Goret-Nicaise M., Braem M., Lambrechts P., Vanherle G. (1993a). Comparative SEM and TEM examination of the ultrastructure of the resin-dentin interdiffusion zone. J Dent Res 72:495-501.
• Van Meerbeek B., Mohrbacher H., Celis JP, Roos JR, Braem M., Lambrechts P., et al. (1993b). Chemical characterization of the resin-dentin interface by micro-Raman spectroscopy. J Dent Res 72:1423-1428.
• Watson TF (1989). A confocal optical microscope study of the morphology of the tooth/restoration interface using Scotchbond 2 dentin adhesive. J Dent Res 68:1124-1131.

Journal of Dental Research, Vol. 76, No. 1, 596-601 (1997)
DOI: 10.1177/00220345970760011101


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Xu, J. Articles by Gilson, D.F.R. Search for Related Content PubMed PubMed Citation Articles by Xu, J. Articles by Gilson, D.F.R. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB 2-HYDROXYETHYL METHACRYLATE CALCIUM COMPOUNDS HYDROXYAPATITE Social Bookmarking                         What's this?