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Expression of Keratinocyte Growth Factor in Periapical Lesions

Dental Branch, University of Texas, Houston Health Science Center

C.M. Flaitz

Dental Branch, University of Texas, Houston Health Science Center

I.C. Mackenzie

Department of Cariology, School of Dentistry, University of Michigan, 1011 N. University, Ann Arbor, Michigan 48109-1078

The epithelial proliferation associated with inflammatory periapical lesions and with periapical cyst formation represents an interesting but poorly understood pathological change. Keratinocyte growth factor (KGF) is a recently identified growth factor that is produced by stromal fibroblasts and acts specifically to stimulate epithelial growth and differentiation. To investigate its possible role in the activation of the normally quiescent rests of Malassez, we examined the expression of KGF by in situ hybridization of sections of normal periodontal ligament (PDL) and of 12 periapical granulomas or cysts. Normal PDL and periapical granulomas with scant inflammatory infiltration showed few cells expressing message for KGF. However, KGFexpressing cells were found in the connective tissue stroma close to dense foci of inflammatory cells and to proliferating epithelial elements and cystic epithelial linings. Examination of tissues by the reverse-transcription polymerase chain reaction (RT-PCR) showed KGF expression in 4 specimens of periapical lesions but low or undetectable levels in normal PDL. These observations suggest that the induction of KGF expression in the stromal cells of periapical lesions may play an important role in stimulating the epithelial proliferation associated with cyst formation.

Key Words: keratinocyte growth factor • periapical lesions • inflammatory cysts • RT/PCR • in situ hybridization.

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Journal of Dental Research, Vol. 75, No. 9, 1658-1663 (1996)
DOI: 10.1177/00220345960750090701


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gao, Z. Articles by Mackenzie, I.C. Search for Related Content PubMed PubMed Citation Articles by Gao, Z. Articles by Mackenzie, I.C. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?