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Expression of Adhesion Molecules during Tooth Resorption in Feline Teeth: A Model System for Aggressive Osteoclastic Activity

Department of Periodontics/Prevention/Geriatrics

T.K. Grove

The Florida Veterinary Dental Clinic, 875 17th Street, Vero Beach, Florida 32960

C. Strayhorn

Department of Periodontics/Prevention/Geriatrics

M.J. Somerman

Department of Periodontics/Prevention/Geriatrics, Department of Pharmacology, School of Medicine, University of Michigan, 1011 N. University, Ann Arbor, Michigan 48109-1078

Tooth resorption, a common feline dental problem, is often initiated at the cemento-enamel junction and hence is called cat 'neck' lesion. Studies have demonstrated that osteoclasts/odontoclasts are increased and activated at resorption sites, and that areas of resorption are partly repaired by formation of tissues resembling bone, cementum, and possibly dentin. However, the cellular/molecular mechanisms/factors involved in resorption and repair are unknown. In this study of tissues from cats with 'neck' lesions, we used specific antibodies and immunohistochemical analyses to examine adhesion molecules associated with mineralized tissues, bone sialoprotein (BSP) and osteopontin (OPN), and a cell-surface receptor linked with these molecules, _vβ₃, for their localization in these lesions. In addition, to determine general cellular activity during repair, we performed in situ hybridization using a type I collagen riboprobe.

Results showed OPN localized to resorption fronts and reversal lines, while BSP was localized to reversal lines. However, some osteoclasts and odontoblasts "sat" on mineralized surfaces not associated with OPN. The cell-surface receptor, _vβ ₃, was localized to surfaces of osteoclasts/odontoclasts. Type I collagen mRNA was expressed where osteoblasts attempted to repair mineralized tissue. In contrast, odontoblasts did not express mRNA for type I collagen. This study suggests that osteoclastic resorption is the predominant activity in 'neck' lesions and that this activity was accompanied, at least in part, by increased concentrations of OPN and an associated integrin, _vβ₃, at resorption sites. Lack of collagen expression by odontoblasts indicates that odontoblasts do not play an active role in attempts at repair.

Key Words: vβ3 • bone sialoprotein • odontoclasts • osteoclasts • osteopontin.

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Journal of Dental Research, Vol. 75, No. 9, 1650-1657 (1996)
DOI: 10.1177/00220345960750090601


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Shigeyama, Y. Articles by Somerman, M.J. Search for Related Content PubMed PubMed Citation Articles by Shigeyama, Y. Articles by Somerman, M.J. Social Bookmarking                         What's this?