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Identification of the Fas Antigen in Human Gingiva

First Department of Oral Surgery, Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

Y. Muraki

First Department of Oral Surgery , Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

J. Fukuda

First Department of Oral Surgery, Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

T. Haneji

First Department of Oral Anatomy, Kyushu Dental College, Manazuru, Kokurakita-ku, Kitakyusyu 803, Japan

N. Kobayashi

Department of Virology and Parasitology, Yamaguchi University, School of Medicine, Kogushi, Ube, Yamaguchi 755, Japan

The Fas antigen is a cell-surface glycoprotein that mediates apoptosis from the cell surface into the cytoplasm. Polyclonal antibody (Fas D) was raised against a synthetic polypeptide selected from the extracellular part of the human Fas antigen (amino acid residues 104-114) and was used to detect the Fas antigen in human gingiva. Biopsy specimens of human gingiva were prepared, and the paraffin sections were reacted with the Fas D antibody by an immunohistochemical method. The antibody localized to the prickle-cell layer and to granular layer keratinocytes of human gingiva. Proteins were also prepared from human gingiva and subjected to SDS-PAGE, followed by Western-blotting analysis with the Fas D antibody. The antibody interacted with a band corresponding to an estimated molecular weight of 35 kDa. The incidence of the immunoreactive 35-kDa protein was detected in the gingiva of 90% of the 20 individuals examined. The Fas antigen detected in human gingiva may be related to the physiological turnover of oral mucosa.

Key Words: Fas antigen • human gingiva • apoptosis

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Journal of Dental Research, Vol. 75, No. 6, 1353-1357 (1996)
DOI: 10.1177/00220345960750060501


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Yoshioka, C. Articles by Kobayashi, N. Search for Related Content PubMed PubMed Citation Articles by Yoshioka, C. Articles by Kobayashi, N. Social Bookmarking                         What's this?