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Effect of Sucrose Monolaurate on Acid Production, Levels of Glycolytic Intermediates, and Enzyme Activities of Streptococcus mutans NCTC 10449

Department of Oral Biochemistry, Tohoku University School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-77, Japan

C.F. Schachtele

Department of Oral Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455

T. Yamada

Department of Oral Biochemistry, Tohoku University School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-77, Japan

We studied the mechanism by which the antimicrobial compound sucrose monolaurate inhibits Streptococcus mutans NCTC 10449 by determining its effect on the rate of acid production from glucose and sucrose and the intracellular and extracellular levels of glycolytic intermediates. Sucrose monolaurate was more effective than either sodium laurate or sodium fluoride in inhibiting acid production at pH 7.0 from glucose. Inhibition of acid production was the same when either glucose or sucrose was the carbon source and in the presence or absence of oxygen. Quantitative analysis of various glycolytic intermediates revealed that the steps inhibited by sucrose monolaurate were the reactions catalyzed by phosphofructokinase and glyceraldehyde 3-phosphate dehydrogenase and/or phosphoglycerate kinase. Since the activities of these enzymes in cell-free extracts were not decreased by the addition of sucrose monolaurate, the inhibition of acid production could not be ascribed to direct effects on the enzymes. A decrease in the rate of acid production with corresponding elevations in the extracellular levels of glycolytic intermediates indicates that sucrose monolaurate inhibits S. mutans by altering the permeability of the cell membrane, which causes a loss of important metabolites.

Key Words: glycolysis • sucrose monolaurate • Streptococcus mutans

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Journal of Dental Research, Vol. 74, No. 9, 1613-1617 (1995)
DOI: 10.1177/00220345950740091801


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Iwami, Y. Articles by Yamada, T. Search for Related Content PubMed PubMed Citation Articles by Iwami, Y. Articles by Yamada, T. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB DODECANOIC ACID SODIUM FLUORIDE SUCROSE Social Bookmarking                         What's this?