Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Nakajima, J.
Right arrow Articles by Harada, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakajima, J.
Right arrow Articles by Harada, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Hypertriglyceridemia associated with Tumor Necrosis Factor-{alpha} in Hamster Cheek-pouch Carcinogenesis

J. Nakajima

Department of Oral & Maxillofacial Surgery I, Matsumoto Dental College, Shiojiri, Nagano 399-07, Japan

M. Mogi

Department of Oral Biochemistry, Matsumoto Dental College, Shiojiri, Nagano 399-07, Japan

T. Kage

Department of Oral & Maxillofacial Surgery I, Matsumoto Dental College, Shiojiri, Nagano 399-07, Japan

T. Chino

Department of Oral & Maxillofacial Surgery I, Matsumoto Dental College, Shiojiri, Nagano 399-07, Japan

M. Harada

Department of Oral Biochemistry, Matsumoto Dental College, Shiojiri, Nagano 399-07, Japan

We demonstrated for the first time that 9,10-dimethyl-l,2-benzanthracene (DMBA)-treated hamsters showed hypertriglyceridemia followed by cachexia. Hypertriglyceridemia is believed to be caused in part by the decreased lipoprotein lipase (LPL) activity, and by cytokines such as tumor necrosis factor (TNF)-a. In addition, TNF-a action is associated with the LPL activity. Therefore, we determined the content of triglyceride (TG), LPL, and TNF-a in the serum from DMBA-treated hamsters. Elevated TG concentration in the serum of tumor-bearing hamsters was more remarkable and preceded the increase in other lipids, whereas the activity of LPL, the key enzyme of TG metabolism in vivo, was drastically reduced. TNF-a, known as an endogenous inhibitor of LPL activity, was detected in both the sera and the extract of tumors from DMBA-treated hamsters, whereas it was not detectable in any control samples. Pre-incubation of control sera with exogenous recombinant human TNF-a resulted in a potent inhibition of endogenous LPL activity in a dose-dependent manner in vitro. Therefore, the presence of TNF-a might lead to the increase in plasma TG mediated by LPL in tumor-bearing hamsters.

Key Words: serum triglyceride • lipoprotein lipase • hamster cheek-pouch carcinogenesis • TNF-a

REFERENCES

  • Allain CC, Poon LS, Chan CS, Richmond W., Fu PC (1974). Enzymatic determination of total serum cholesterol. Clin Chem 20:470-475.[Abstract]
  • Barclay M., Skipski VP (1975). Lipoproteins in relation to cancer. Prog Biochem Pharmacol 10:76-111.[Medline] [Order article via Infotrieve]
  • Beutler B., Cerami A. (1986). Cachectin and tumor necrosis factor as two sides of the same biological coin. Nature 320:584-588.[CrossRef][Medline] [Order article via Infotrieve]
  • Bradford MM (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.[CrossRef][Medline] [Order article via Infotrieve]
  • Chisari FV (1977). Immunoregulatory properties of human plasma in very low density lipoproteins. J Immunol 119:2129-2136.[Abstract/Free Full Text]
  • Enerback S., Semb H., Tavernier J., Bjursell G., Olivecrona T. (1988). Tissue-specific regulation of guinea pig lipoprotein lipase; effects of nutritional state and of tumor necrosis factor on mRNA levels in adipose tissue, heart and liver. Gene 64:97-106.[CrossRef][Medline] [Order article via Infotrieve]
  • Fearon KC, Carter DC (1988). Cancer cachexia. Ann Surg 208:1-5.[Medline] [Order article via Infotrieve]
  • Feingold KR, Grunfeld C. (1987). Tumor necrosis factor-alpha stimulates hepatic lipogenesis in the rat in vivo. J Clin Invest 80:184-190.[Medline] [Order article via Infotrieve]
  • Fiser RH, Denniston JC, Beisel WR (1972). Infection with Diplococcus pneumoniae and Salmonella typhimurium in monkeys: changes in plasma lipids and lipoproteins. J Infect Dis 125:54-60.[Medline] [Order article via Infotrieve]
  • Grunfeld C., Palladino MA Jr. (1990). Tumor necrosis factor: Immunologic, antitumor, metabolic and cardiovascular activities. Adv Intern Med 35:45-71.[Medline] [Order article via Infotrieve]
  • Grunfeld C., Gulli R., Moser AH, Gavin LA, Feingold KR (1989). Effect of tumor necrosis factor administration in vivo on lipoprotein lipase activity in various tissues of the rat. J Lipid Res 30:579-585.[Abstract]
  • Holmes S., Dickerson JW (1987). Malignant disease: nutritional implications of disease and treatment. Cancer Metastasis Rev 6:357-381.[Medline] [Order article via Infotrieve]
  • Kannan R., Baker N. (1977). Hypertriglyceridemia in Ehrlich ascites carcinomatous mice: tumor and mouse strain differences. Lipids 12:153-158.[CrossRef][Medline] [Order article via Infotrieve]
  • Kage T., Mogi M., Katsumata Y., Chino T. (1987). Regional lymph node metastasis created by partial excision of carcinomas induced in hamster cheek pouch with 9,10-dimethyl-1,2-benzanthracene. J Dent Res 66:1673-1679.
  • Kage T., Mogi M., Katsumata Y., Yamada T., Chino T. (1988). Lung and cervical lymph-node metastasis after cortisone enhancement in hamster cheek-pouch carcinogenesis. Arch Oral Biol 33:459-461.[Medline] [Order article via Infotrieve]
  • Kaufmann RL, Matson CF, Beisel WR (1976). Hypertriglyceridemia produced by endotoxin: role of impaired triglyceride disposal mechanisms. J Infect Dis 133:548-555.[Medline] [Order article via Infotrieve]
  • Kawakami M., Cerami A. (1981). Studies of endotoxin-induced decrease in lipoprotein lipase activity. J Exp Med 154:631-639.[Abstract/Free Full Text]
  • Kawakami M., Pekala PH, Lane MD, Cerami A. (1982). Lipoprotein lipase suppression in 3T3-L1 cells by an endotoxin-induced mediator from exudate cells. Proc Natl Acad Sci USA 79:912-916.[Abstract/Free Full Text]
  • Kobayashi K., Ogasawara N., Sakaguchi T., Taniuchi K., Matsuoka A. (1989). Serum glycated beta-lipoprotein determined by agarose-gel electrophoresis with nitroblue tetrazolium coloration. Clin Chem 35:177-178.[Free Full Text]
  • Kushiro H., Takano Y., Fukui I. (1975). Determination of lipoprotein lipase activity. Jpn J Clin Pathol 21:110-123.
  • Laurell S., Tibbling G. (1967). Colorimetric micro-determination of free fatty acids in plasma. Clin Chim Acta 16:57-62.[CrossRef][Medline] [Order article via Infotrieve]
  • Mogi M., Kojima K., Nagatsu T. (1984). Detection of inactive or less active forms of tyrosine hydroxylase in human adrenals by a sandwich enzyme immunoassay. Anal Biochem 138:125-132.[Medline] [Order article via Infotrieve]
  • Mogi M., Hiraoka BY, Harada M., Kage T., Chino T. (1986). Analysis and identification of human parotid salivary proteins by micro two-dimensional electrophoresis and Western blot techniques. Arch Oral Biol 31:337-339.[CrossRef][Medline] [Order article via Infotrieve]
  • Mogi M., Harada M., Riederer P., Narabayashi H., Fujita K., Nagatsu T. (1994). Tumor necrosis factor-a increases both in the brain and in the cerebrospinal fluid from Parkinsonian patients. Neurosci Lett 165:208-210.[CrossRef][Medline] [Order article via Infotrieve]
  • Morse JH, Witte LD, Goodman DS (1977). Inhibition of lymphocyte proliferation stimulated by lectins and allogeneic cells by normal plasma lipoproteins. J Exp Med 146:1791-1803.[Abstract/Free Full Text]
  • Nagele U., Hagele EO, Sauer G., Wiedemann E., Lehmann P., Wahlefold AW, et al. (1984). Reagent for the enzymatic determination of serum total triglycerides with improved lipolytic efficiency. J Clin Chem Clin Biochem 22:165-174.[Medline] [Order article via Infotrieve]
  • Rouzer CA, Cerami A. (1980). Hypertriglyceridemia associated with trypanosoma Brucei infection in rabbits: role of defective triglyceride removal. Molec Biochem Parasitol 2:31-38.[CrossRef][Medline] [Order article via Infotrieve]
  • Salley JJ (1954). Experimental carcinogenesis in the cheek pouch of the Syrian hamster. J Dent Res 33:253-262.[Medline] [Order article via Infotrieve]
  • Schwartz J., Suda D., Light G. (1986). Beta carotene is associated with the regression of hamster buccal pouch carcinoma and the induction of tumor necrosis factor in macrophages. Biochem Biophys Res Commun 136:1130-1135.[Medline] [Order article via Infotrieve]
  • Semb H., Peterson J., Tavernier J., Olivecrona T. (1987). Multiple effects of tumor necrosis factor on lipoprotein lipase in vivo. J Biol Chem 262:8390-8394.[Abstract/Free Full Text]
  • Shklar G., Schwartz J. (1988). Tumor necrosis factor in experimental cancer regression with alphatocophenol, beta-carotene, canthaxanthin and algae extract. Eur J Cancer Clin Oncol 24:839-850.[CrossRef][Medline] [Order article via Infotrieve]
  • Takayama M., Itoh S., Nagasaki T., Tanimizu I. (1977). A new enzymatic method for determination of serum choline-containing phospholipids. Clin Chim Acta 79:93-98.[CrossRef][Medline] [Order article via Infotrieve]
  • Thoen CO, Karlson AG, Ellefson RD (1972). Mechanism of changes in serum lipid lipoprotein profiles in the infectious disease. Mayo Clin Proc 47:270-272.[Medline] [Order article via Infotrieve]
  • Tracey KJ, Cerami A. (1994). Tumor necrosis factor: a pleiotropic cytokine and therapeutic target. Ann Rev Med 45:491-503.[CrossRef][Medline] [Order article via Infotrieve]
  • Tracey KJ, Cerami A. (1992). Tumor necrosis factor and regulation of metabolism in infection: role of systemic versus tissue levels. Proc Soc Exp Biol Med 200:233-239.[CrossRef][Medline] [Order article via Infotrieve]
  • Vannier C., Deslex S., Pradines-Figueres A., Ailhaud G. (1989). Biosynthesis of lipoprotein lipase in cultured mouse adipocytes. J Biol Chem 264:13199-13205.[Abstract/Free Full Text]
  • Yamada T., Kage T., Wakano T., Nakajima J., Mogi M., Chino T. (1988). Serum sialic acid alterations in the hamster cheek pouch carcinogenesis. Jpn J Oral Biol 30:695-698.
  • Yamada T., Mogi M., Kage T., Ueda A., Nakajima J., Chino T. (1992). Enhancement by cyclosporin A of metastasis from hamster cheek pouch carcinoma. Arch Oral Biol 37:593-596.[Medline] [Order article via Infotrieve]
  • Yui S., Yamazaki M. (1986). Induction of macrophage growth by lipids. J Immunol 136:1334-1338.[Abstract]

Journal of Dental Research, Vol. 74, No. 9, 1558-1563 (1995)
DOI: 10.1177/00220345950740090901
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CJASNHome page
B. C.H. Kwan, M. A. Murtaugh, and S. Beddhu
Associations of Body Size with Metabolic Syndrome and Mortality in Moderate Chronic Kidney Disease
Clin. J. Am. Soc. Nephrol., September 1, 2007; 2(5): 992 - 998.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Nakajima, J.
Right arrow Articles by Harada, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakajima, J.
Right arrow Articles by Harada, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?