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Cellular Response to Metallic Ions Released from Nickel-Chromium Dental Alloys

Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama

L.C. Lucas

Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, Alabama

Concerns exist over the potential release of elevated levels of metal ions such as Ni and Be from Ni-Cr dental casting alloys, due to their susceptibility to accelerated corrosion. In this investigation, we evaluated the release of metal ions from four commercial Ni-Cr alloys, representing a range of compositions, in three-day cell culture tests. Metal ion release, as measured by atomic absorption spectroscopy, was correlated to changes in cellular morphology, viability, and proliferation. The results showed that the test alloys and their corrosion products did not affect cellular morphology or viabilities, but did decrease cellular proliferation. The types and amounts of metal ions released, which corresponded to the alloys' reported surface and corrosion properties, also correlated to observed decreases in cellular proliferation after 72 h. Neptune, which caused the smallest decrease in cellular proliferation as compared with control cells, released the lowest amount of corrosion products, due to its corrosion-resistant, high-Cr-Mo-containing, homogeneous surface oxide. The other test alloys, which were susceptible to accelerated corrosion processes, released higher levels of metal ions that correlated to larger decreases in thymidine incorporation. Metal ion levels increased with test time for all alloys but were not proportional to bulk alloy compositions. Ni ions were released at slightly higher than bulk alloy compositions, while Be was released at from four to six times that of bulk alloy compositions. The elevated release of Be ions was associated with reduced cellular proliferation. Other alloying elements were released at levels similar to or lower than bulk levels. Further research is needed to evaluate possible synergistic effects of released metal ions, especially Ni and Be ions, on cellular activities and functions.

Key Words: dental alloys • Ni-Cr alloys • cell culture • fibroblasts • toxicity

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Journal of Dental Research, Vol. 74, No. 8, 1521-1527 (1995)
DOI: 10.1177/00220345950740081401


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Bumgardner, J.D. Articles by Lucas, L.C. Search for Related Content PubMed PubMed Citation Articles by Bumgardner, J.D. Articles by Lucas, L.C. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB BERYLLIUM, ELEMENTAL CHROMIUM COMPOUNDS CHROMIUM, ELEMENTAL MOLYBDENUM NICKEL, ELEMENTAL Social Bookmarking                         What's this?