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Journal of Dental Research
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Evidence for the Role of Nitric Oxide in the Circulation of the Dental Pulp

Z. Lohinai

Cerebrovascular Research Center, Department of Neurology, University of Pennsylvania, Philadelphia, USA, Experimental Research Department-2nd Institute of Physiology, Semmelweis University of Medicine, 1082 Budapest, Üllói út 78/A, Hungary

I. Balla

Cerebrovascular Research Center, Department of Neurology, University of Pennsylvania, Philadelphia, USA, Experimental Research Department-2nd Institute of Physiology, Semmelweis University of Medicine, 1082 Budapest, Üllói út 78/A, Hungary

J. Marczis

Cerebrovascular Research Center, Department of Neurology, University of Pennsylvania, Philadelphia, USA, Experimental Research Department-2nd Institute of Physiology, Semmelweis University of Medicine, 1082 Budapest, Üllói út 78/A, Hungary

Z. Vass

Cerebrovascular Research Center, Department of Neurology, University of Pennsylvania, Philadelphia, USA, Experimental Research Department-2nd Institute of Physiology, Semmelweis University of Medicine, 1082 Budapest, Üllói út 78/A, Hungary

A.G.B. Kovach

Cerebrovascular Research Center, Department of Neurology, University of Pennsylvania, Philadelphia, USA, Experimental Research Department-2nd Institute of Physiology, Semmelweis University of Medicine, 1082 Budapest, Üllói út 78/A, Hungary

Many authors have studied the hemodynamics of the dental pulp; however, there are scarcely any data regarding the involvement of the L-arginine/nitric oxide pathway in the regulatory mechanism. Thus, we have examined the physiological effects of (1) NG-nitro-L-arginine as an inhibitor of nitric oxide synthesis and (2) the nitric oxide donor 3-morpholinosydnonimine on blood flow and vascular resistance in the canines of anesthetized cats to study the potential involvement of nitric oxide in the regulation of dental vascular homeostasis. Mean arterial blood pressure, heart rate, blood gases, pH, cardiac output, and tissue blood flow were determined prior to and 15 min after i.v. administration of either NG-nitro-L-arginine (30 mg/kg, n = 9) or 3-morpholinosydnonimine (1 mg/kg, n = 7). Blood flow was measured by radioactive-labeled microspheres. There were no significant differences in baseline parameters between the two groups of cats. The dental pulp blood flow decreased to 53 ± 13% (p < 0.01) of the control level after NG-nitro-L-arginine administration, while it decreased only slightly (to 82 ± 12%) after 3-morpholinosydnonimine administration. The dental pulp's vascular resistance increased to 367 ± 69% (p < 0.01) of the control level after NG-nitro-L-arginine, while it decreased to 73 ± 10% (p < 0.05) of control after 3-morpholinosydnonimine. We found that the L-arginine/nitric oxide pathway plays an important role in the regulation of pulpal blood circulation. A nitric-oxide-dependent basal vasodilator tone exists in the dental pulp; furthermore, since the dental pulp circulation is sensitive to exogenous nitric oxide, the stimulated release of endogenous nitric oxide may also be involved in the control of the dental pulp vascular tone.

Key Words: nitric oxide • blood flow • vascular resistance, • dental pulp, cat

REFERENCES

  • Berkenboom G., Fang YZ, Unger PH, Fontaine J. (1990). Effects of in vivo SIN1 treatment on nitrovasodilator relaxation and on EDRF-mediated responses in rat aorta. J Cardiovasc Pharmacol 16:636-640.[Medline] [Order article via Infotrieve]
  • Bolanos OR, Seltzer S. (1981). Cyclic AMP and cyclic GMP quantitation in pulp and periapical lesions and their correlation with pain. J Endod 7:268-271.[Medline] [Order article via Infotrieve]
  • Buckberg GD, Luck JC, Payne DB, Hoffman JIE, Archie JP, Fixler DE (1971). Some sources of error in measuring regional blood flow with radioactive microspheres. J Appl Physiol 31:598-604.[Free Full Text]
  • Casasco A., Calligaro A., Casasco M., Springall DR, Polak JM, Poggi P., et al. (1990). Peptidergic nerves in human dental pulp. An immunocytochemical study. Histochemistry 95:115-121.[Medline] [Order article via Infotrieve]
  • Casasco A., Calligaro A., Casasco M., Springall DR, Tenti P., Marchetti C., et al. (1991). Immunohistochemical localization of endothelin-like immunoreactivity in human tooth germ and mature dental pulp. Anat Embryol Berl 183:515-520.[Medline] [Order article via Infotrieve]
  • Dinerman JL, Lowenstein ChJ, Snyder SH (1993). Molecular mechanisms of nitric oxide regulation. Potential relevance to cardiovascular disease. Circ Res 73:217-230.[Free Full Text]
  • Feelisch M., Noack E. (1987). Nitric oxide (NO) formation from nitrovasodilators occurs independently of hemoglobin or non-heme iron. Eur J Pharmacol 142:465-469.[CrossRef][Medline] [Order article via Infotrieve]
  • Feelisch M., Ostrowski J., Noack E. (1989). On the mechanism of NO release from sydnonimines. J Cardiovasc Pharmacol 14(Suppl 11):S13-S22.
  • Friedman S., Liu M., Izawa T., Moynihan M., Dorscher-Kim J., Kim S. (1992). Effects of CO2 laser irradiation on pulpal blood flow. Proc Finn Dent Soc 88(Suppl 1):167-171.
  • Furchgott RF, Zawadzki JV (1980). The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 288:373-376.[CrossRef][Medline] [Order article via Infotrieve]
  • Gardiner SM, Compton AM, Bennett T., Palmer RMJ, Moncada S. (1990). Control of regional blood flow by endothelium-derived nitric oxide. Hypertension 15:486-492.[Abstract/Free Full Text]
  • Gilbert TM, Pashley DH, Anderson RW (1992). Response of pulpal blood flow to intra-arterial infusion of endothelin. J Endod 18:228-231.[Medline] [Order article via Infotrieve]
  • Gross S., Stuehr DJ, Aisaka K., Jaffe EA, Levi R., Griffith OW (1990). Macrophage and endothelial cell nitric oxide synthesis: cell-type selective inhibition by NGaminoarginine, NG-nitroarginine and NG-methylarginine. Biochem Biophys Res Commun 170:96-103.[CrossRef][Medline] [Order article via Infotrieve]
  • Heyeraas KJ, Kvinnsland I. (1992). Tissue pressure and blood flow in pulpal inflammation. Proc Finn Dent Soc 88(Suppl 1):393-401.
  • Heymann M., Payne BD, Hoffman JF, Rudolf A. (1977). Blood flow measurement with radionuclide-labeled particles. Prog Cardiovasc Dis 20:55-79.[CrossRef][Medline] [Order article via Infotrieve]
  • Himbert D., Jaeger P., Himbert C., Steg PG, Juliard JM, Cohen-Solal A., et al. (1991). Acute hemodynamic effects of SIN-1 vs. isosorbide dinitrate: a double-blind, crossover study in stable chronic left ventricular failure. J Cardiovasc Pharmacol 27:279-286.
  • Holtz J., Bassenge E., Kolin A. (1978). Hemodynamic and myocardial effects of long-lasting venodilation in the conscious dog: analysis of molsidomine in comparison with nitrates. Basic Res Cardiol 73:469-481.[CrossRef][Medline] [Order article via Infotrieve]
  • Humphries RG, Carr RD, Nicol AK, Tomlinson W., Connor SE (1991). Coronary vasoconstriction in the conscious rabbit following intravenous infusion of L-NG-nitro-arginine. Br J Pharmacol 102:565-566.[Medline] [Order article via Infotrieve]
  • Kerezoudis NP, Olgart L., Edwall L. (1993a). Differential effects of nitric oxide synthesis inhibition on basal blood flow and antidromic vasodilation in rat oral tissues. Eur J Pharmacol 241:209-219.[CrossRef][Medline] [Order article via Infotrieve]
  • Kerezoudis NP, Olgart L., Fried K. (1993b). Localization of NADPH-diaphorase activity in the dental pulp, periodontium and alveolar bone of the rat. Histochemistry 100:319-322.[Medline] [Order article via Infotrieve]
  • Kim S. (1985). Regulation of pulpal blood flow. J Dent Res 64:590-596.[Medline] [Order article via Infotrieve]
  • Kim S. (1990). Neurovascular interactions in the dental pulp in health and inflammation. J Endod 16(2):48-53.[Medline] [Order article via Infotrieve]
  • Kim S., Dorscher-Kim J. (1989). Hemodynamic regulation of the dental pulp in a low compliance environment. J Endod 15:404-408.[Medline] [Order article via Infotrieve]
  • Kim S, Fan F, Chen RYZ, Simchon S, Schuessler GB, Chien S (1980). Effect of changes in systemic hemodynamic parameters on pulpal hemodynamics. J Endod 6:394-399. Kim S, Dorscher-Kim J, Liu M-T, Trowbridge HO (1988). Biphasic pulp blood-flow response to substance P in the
  • dog as measured with a radiolabelled, microsphere injection method. Arch Oral Biol 33:305-309.
  • Kim S., Liu M., Simchon S., Dorscher-Kim JE (1992a). Effects of selected inflammatory mediators on blood flow and vascular permeability in the dental pulp. Proc Finn Dent Soc 88(Suppl 1):387-392.
  • Kim S., Dorscher-Kim JE, Liu M., Grayson A. (1992b). Functional alterations in pulpal microcirculation in response to various dental procedures and materials. Proc Finn Dent Soc 88(Suppl 1):65-71.
  • Kovach AGB, Szabo CS, Benyo Z., Csaki CS, Greenberg JH, Reivich M. (1992). Effects of NG-nitro-L-arginine and L-arginine on regional cerebral blood flow in the cat. J Physiol 449:183-196.[Abstract/Free Full Text]
  • Kvinnsland S., Heyeraas K., Ofjord ES (1989). Effect of experimental tooth movement on periodontal and pulpal blood flow. Eur J Orthod 11:200-205.[Abstract/Free Full Text]
  • Lambert LE, Whitten JP, Baron BM, Cheng HC, Doherty NS, McDonald IA (1991). Nitric oxide synthesis in the ens, endothelium and macrophages differs in its sensitivity to inhibition by arginine analogues. Life Sci 48:69-75.[CrossRef][Medline] [Order article via Infotrieve]
  • Leon RL, Path MG, Meyer MW (1978). Blood flow and oxygen consumption in steroid-treated dental pulps. Oral Surg Oral Med Oral Pathol 45:784-788.[Medline] [Order article via Infotrieve]
  • Liu M., Kim S., Park DS, Markowitz K., Bilotto G., Dorscher-Kim J. (1990). Comparison of the effects of intra-arterial and locally applied vasoactive agents on pulpal blood flow in dog canine teeth determined by laser Doppler velocimetry. Arch Oral Biol 35:405-410.[Medline] [Order article via Infotrieve]
  • Malik AB, Kaplan JE, Saba TM ( 1976). Reference sample method for cardiac output and regional blood flow determinations in the rat. J Appl Physiol 40:472-475.[Abstract/Free Full Text]
  • Meyer MW, Path MG (1979). Blood flow in the dental pulp of dogs determined by hydrogen polarography and radioactive microsphere methods. Arch Oral Biol 24:601-605. Miller VM, Vanhoutte PM (1990). Relaxations to SIN-1, nitric oxide, and sodium nitroprusside in canine arteries and veins. J Cardiovasc Pharmacol 14:67-71.
  • Moncada S., Palmer RMJ, Higgs EA (1991). Nitric oxide
  • physiology, pathophysiology, and pharmacology. Pharmacolog Rev 43:109-142.
  • Moore PK, Al-Swayeh OA, Chong NWS, Evans RA, Gibson A. (1990). L-NG-nitro arginine (L-NOARG), a novel, L-arginine-reversible inhibitor of endothelium-dependent vasodilation in vitro. Br J Pharntacol 99:408-412.
  • Narhi M (1989). Interaction between the autonomic and sensory nerves in the dental pulp. Proc Finn Dent Soc 85:389-393. Olgart LM, Edwall B, Gazelius B (1989). Neurogenic mediators in control of pulpal blood flow. J Endod 15:409-412. Path MG, Meyer MW (1977). Quantification of pulpal blood flow in developing teeth of dogs. J Dent Res 56:1245-1254. Raab WH (1990). Assessment of tooth pulp reaction to dental materials in an animal experiment. Dtsch Zahnärztl Z 45:345-348.
  • Raab WH (1992). Temperature related changes in pulpal microcirculation. Proc Finn Dent Soc 88(Suppl 1):469-479. Sasano T, Kuriwada S, Sanjo D (1989). Arterial blood pressure regulation of pulpal blood flow as determined by laser doppler. J Dent Res 68:791-795.
  • Sproles AC, Schilder H, Schaffer LD (1979). Cyclic AMP and cyclic GMP concentrations in normal and pulpitic human dental pulps (annotation). J Dent Res 58:2369. Takahashi K (1985). Vascular architecture of dog pulp using corrosion resin cast examined under a scanning electron microscope. J Dent Res 64(Spec Iss):579-584.
  • Vanhoutte PM (1989). Molsidomine is a donor of EDRF. J Cardiovasc Pharmacol 14(Suppl 11):S139-S140. Vongsavan N, Matthews B (1992). The vascularity of dental pulp in cats. J Dent Res 71:1913-1915.
  • Van de Voorde J. (1991). Mechanisms involved in the development of tolerance to nitrovasodilators. J Cardiovasc Pharmacol 17:304-308.
  • Van de Voorde J., Claeys M., Leusen I. (1989). Relaxations to endothelium-derived relaxing factor and the metabolite of molsidomine, SIN-1, in the aorta and the hindquarters of the rat. J Cardiovasc Pharmacol 14:55-61.
  • Wang Y.-X., Pang CCY (1990). Pressor effect of NG-nitro-L-arginine in pentobarbital-anesthetized rats. Life Sci 47:2217-2224.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 74, No. 8, 1501-1506 (1995)
DOI: 10.1177/00220345950740081101
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