This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ye, J. Articles by Terranova, V.P. Search for Related Content PubMed PubMed Citation Articles by Ye, J. Articles by Terranova, V.P. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?

Isolation, Purification, and Partial Characterization of an Autocrine Periodontal Ligament Cell Chemotactic Factor

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 West 168th Street, New York, New York 10032, Bronx VAMC, 130 West Kingsbridge Rd., Bronx, New York 10468

F. Nishimura

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 West 168th Street, New York, New York 10032, Bronx VAMC, 130 West Kingsbridge Rd., Bronx, New York 10468

R. Orman

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 West 168th Street, New York, New York 10032, Bronx VAMC, 130 West Kingsbridge Rd., Bronx, New York 10468

V.P. Terranova

Laboratory of Tumor Biology and Connective Tissue Research, Columbia University, 630 West 168th Street, New York, New York 10032, Bronx VAMC, 130 West Kingsbridge Rd., Bronx, New York 10468

Periodontal ligament (PDL) cells are believed to play a critically important role in the regeneration of the periodontium. We have suggested that polypeptide growth factors can enhance periodontal regeneration by stimulating PDL cell chemotaxis and mitogenesis. This manuscript describes the identification of a novel chemotactic factor isolated from human PDL cells which we named PDL-CTX. PDL-CTX induces the directed migration of human PDL cells in vitro and was found to be a more potent chemotactic agent than other known growth factors. Additionally, PDL-CTX has no chemotactic effect on gingival fibroblasts or gingival epithelial cells. Both tryptic digestion and boiling abolished PDL-CTX's biological activity. The designed purification method included Mono-S cation exchange, heparin-sepharose affinity, and microbore reverse-phase HPLC. The purified factor has a relative molecular weight of approximately 7000 daltons based on sodium dodecyl sulfate (SDS) gel analysis. The amino acid composition and partial amino acid sequence were determined from HPLCpurified material. These were determined to be unique. Further investigation of the biological functions of PDL-CTX on PDL cells and other ligament cells should help improve our understanding of ligament repair.

Key Words: growth factor • chemotaxis • autocrine growth factor

REFERENCES

• Armelin HA, Armelin MCS (1987). The interaction of peptide growth factors and oncogenes. In: Oncogenes, genes and growth factors. Guroff G, editor. New York: John Wiley and Sons, pp. 331-374.
• Clark RAF (1988). Overview and general consideration of wound repair. In: The molecular and cellular biology of wound repair. Clark RAF, Henson PM, editors. New York: Plenum Press, pp. 3-19.
• Douglas CW, Gillings D., Sollecito W., Gammon M. (1983). National trends in the prevalence and severity of the periodontal diseases. J Am Dent Assoc 107:403-412.[Abstract]
• Froesch ER, Schmid C., Schwander J., Zapf J. ( 1985). Actions of insulin-like growth factors. Ann Rev Physiol 47:443-467. Gospodarowicz D., Mascoglin S., Cheng J., Lui G., Bohlen P. (1985). Isolation of pituitary fibroblast growth factor by fast protein liquid chromatography (FPLC): Partial chemical and biological characterization. J Cell Physiol 122:323-332. Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 222:680-685.[CrossRef]
• Liotta LA, Mandler R., Murano G., Katz DA, Gordon RK, Chiang PK, et al. (1986). Tumor cell autocrine motility factor. Proc Natl Acad Sci USA 83:3302-3306.[Abstract/Free Full Text]
• Loesche WJ (1982). The bacterial etiology of dental decay and periodontal disease: the specific plaque hypothesis. Clin Dent 2:1-54.
• Lynch SE (1991). Interaction of growth factors in tissue repair. In: Clinical and experimental approaches to dermal and epidermal repair: normal and chronic wound. Barbul A, editor. New York: Alan Liss, pp. 341-357.
• Nishimura F., Terranova VP (1995). Comparative study of the chemotactic responses of periodontal ligament cells and gingival fibroblasts to polypeptide growth factors. J Dent Res, submitted.
• Nyman S., Gottlow J., Karring T., Linde J. (1982). The regenerative potential of the periodontal ligament. An experimental study in the monkey. J Clin Periodontol 9:257-265.[Medline] [Order article via Infotrieve]
• Ragnarsson B., Carr G., Daniel JC (1985). Isolation and growth of human periodontal ligament cells in vitro. J Dent Res 64:1026-1031.
• Ross R., Rainer EW, Bowen-Pope DF (1986). The biology of platelet-derived growth factor. Cell 46:155-169.[CrossRef][Medline] [Order article via Infotrieve]
• Schiffmann E., Gallin JE (1979). Biochemistry of phagocyte chemotaxis. In: Current topics in cellular regulation. Horecker BL, Stadman ER, editors. New York: Academic Press, Inc., pp. 203-216.
• Selvig KA (1983). Current concepts of connective tissue attachment to diseased tooth surfaces. J Biol Buccale 11:79-94. Somerman MJ, Argraves WS, Foster RA, Dickerson K., Norris K., Sauk JJ (1991). Cell attachment activity of cementum: bone sialoprotein II identified in cementum. J Periodont Res 26:10-16. Sporn MB, Roberts AB (1986). Peptide growth factors and inflammation, tissue repair and cancer. J Clin Invest 78:329-332.[Medline] [Order article via Infotrieve]
• Stone KL, LoPresti MB, Crawford JM, DeAngelis R., Williams K. (1989). Enzymatic digestion of proteins and HPLC peptide isolation. In: A practical guide to protein and peptide purification for microsequencing. Matsudaira PT, editor. San Diego: Academic Press, pp. 31-47.
• Tanner ACR, Haffer C., Bratthall GT, Visconti RA, Socransky SS (1979). A study of the bacteria associated with advancing periodontitis in man. J Clin Periodontol 6:278-289.[CrossRef][Medline] [Order article via Infotrieve]
• Terranova VP, Wikesjo UME (1987). Extracellular matrices and polypeptide growth factors as mediators of functions of cells of the periodontium: A review. J Periodontol 58:371-380.[Medline] [Order article via Infotrieve]
• Terranova VP, Wikesjo UME (1988). Chemotaxis of human periodontal ligament cells to various biological response modifiers. Adv Dent Res 2:215-222.[Abstract/Free Full Text]
• Terranova VP, Franzetti C., Hic S., Lyall RM, Wikesjo UME, Baker PJ, et al. (1986). A biochemical approach to periodontal regeneration: tetracycline treatment of dentin promotes fibroblast adhesion and growth. J Periodont Res 21:330-337.[Medline] [Order article via Infotrieve]
• Terranova VP, Hic S., Franzetti L., Lyall RM, Wikesjo UME (1987). A biochemical approach to periodontal regeneration: AFSCM: assay for specific cell migration. J Periodontol 58:247-257.[Medline] [Order article via Infotrieve]
• Terranova VP, Odziemiec C., Tweden KS, Spadone DP (1989). Repopulation of dentin surfaces by periodontal ligament cells and endothelial cells. Effect of basic fibroblast growth factor. J Periodontol 60:293-301.[Medline] [Order article via Infotrieve]
• Terranova VP, Price RM, Morishita M. (1991). Periodontal regeneration: myth or reality? J Int Dent 41:287-294. Yonemura K., Narayanan AS, Miki Y., Page RC, Okada H. (1992). Isolation and partial characterization of a growth factor from human cementum. Bone Min 18:187-198.

Journal of Dental Research, Vol. 74, No. 6, 1303-1309 (1995)
DOI: 10.1177/00220345950740061001


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F. Nishimura, V.P. Terranova, T. Sawa, and Y. Murayama Migration Inhibitory Factor Related Protein-8 (MRP-8) is an Autocrine Chemotactic Factor for Periodontal Ligament Cells Journal of Dental Research, June 1, 1999; 78(6): 1251 - 1255. [Abstract] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ye, J. Articles by Terranova, V.P. Search for Related Content PubMed PubMed Citation Articles by Ye, J. Articles by Terranova, V.P. Pubmed/NCBI databases Substance via MeSH Social Bookmarking                         What's this?