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Saliva Protein Binding to Layers of Oral Streptococci in vitro and in vivo
J.D. Rudney
Department of Oral Science, School of Dentistry, 515 Delaware St., SE, University of Minnesota, Minneapolis, Minnesota 55455
Z. Ji
Department of Oral Science, School of Dentistry, 515 Delaware St., SE, University of Minnesota, Minneapolis, Minnesota 55455
C.J. Larson
Department of Oral Science, School of Dentistry, 515 Delaware St., SE, University of Minnesota, Minneapolis, Minnesota 55455
W.F. Liljemark
Department of Diagnostic and Surgical Science, School of Dentistry, 515 Delaware St., SE, University of Minnesota, Minneapolis, Minnesota 55455
K.L. Hickey
Department of Oral Science, School of Dentistry, 515 Delaware St., SE, University of Minnesota, Minneapolis, Minnesota 55455
This paper reports a system for measuring saliva protein binding to oral streptococci. Enamel chips with layers of Streptococcus gordonii Blackburn or Streptococcus oralis 10557 were incubated in vitro with whole saliva from eight persons. Blackburn bound significantly more amylase than 10557; no strain differences were seen for lysozyme or lactoferrin. There were significant correlations between saliva and bound amylase and lactoferrin. Blackburn and 10557 chips were then placed in ten subjects. Sites included the buccal left and right upper premolars and molars (UL, UR), labial upper central incisors (UC), and lingual lower central incisors (LL). That study was repeated three months later; chips with Streptococcus sanguis 13379 were also placed then. Blackburn bound significantly more amylase than the other strains. Blackburn and 10557 both bound the most amylase at UL and UR, and the least amylase at UC. However, strain 13379 bound less amylase at UL. That strain also bound significantly less sIgA at UL. All three strains bound the least sIgA at UC. Lysozyme and lactoferrin binding showed few differences among sites or strains. Bound protein concentrations were significantly correlated across sites and strains within subjects, but not correlated with whole saliva. Strain differences may reflect species differences in amylase binding, or differences in species-specific sIgA titers. Site differences may indicate local variation in protein availability. Differences between chip correlations with whole saliva in vitro and in vivo suggest that the salivary film may be modified as it flows over tooth surfaces.
Key Words: saliva • amylase • secretory IgA • lysozyme • lactoferrin • Streptococcus gordonii • Streptococcus oralis • Streptococcus sanguis
REFERENCES
- Arnold R., Brewer M., Gauthier J. (1980). Bactericidal activity of human lactoferrin: sensitivity of a variety of microorganisms. Infect Immun 28:893-898.[Abstract/Free Full Text]
- Arnold RR, Russell J., Champion W., Brewer M., Gauthier J. (1982). Bactericidal activity of human lactoferrin: differentiation from stasis of iron deprivation. Infect Immun 35:792-799.[Abstract/Free Full Text]
- Caufield PW, Walker TM (1989). Genetic diversity within Streptococcus mutans evident from chromosomal DNA restriction fragment polymorphisms. J Clin Microbiol 27:274-278.[Abstract/Free Full Text]
- Dawes C., Watanabe S., Biglow-Lecomte P., Dibdin G. (1989). Estimation of the velocity of the salivary film at some different locations in the mouth. J Dent Res 68:1479-1482.
- Douglas CWI (1990). Characterization of the a-amylase receptor of Streptococcus gordonii NCTC 7868. J Dent Res 69:1746-1752.
- Douglas CWI, Pease AA, Whiley RA (1990). Amylase-binding as a discriminator among oral streptococci. FEMS Microbiol Lett 66:193-198.
- Drexler G., Eichinger A., Wolf C., Sieghart W. (1986). A rapid and simple method for efficient coating of microtiter plates using low amounts of antigen in the presence of detergent. J Immunol Meth 95:117-122.[CrossRef][Medline]
[Order article via Infotrieve]
- Frandsen EVG, Pedrazzoli V., Kilian M. (1991). Ecology of viridans streptococci in the oral cavity and pharynx. Oral Microbiol Immunol 6:129-133.[Medline]
[Order article via Infotrieve]
- Gibbons R., Etherden I. (1985). Albumin as a blocking agent in studies of streptococcal adsorption to experimental salivary pellicles. Infect Immun 50:592-594.[Abstract/Free Full Text]
- Gibbons R., Hay D., Schlesinger D. (1991). Delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of Streptococcus gordonii to apatitic surfaces. Infect Immun 59:2948-2954.[Abstract/Free Full Text]
- Huang BY, Prakobphol A., Fisher SJ (1992). Effect of receptor aggregation on bacterial adherence (abstract). J Dent Res 71:164.
- Iacono VJ, MacKay B., DiRienzo S., Pollock J. (1980). Selective antibacterial properties of lysozyme for oral microorganisms. Infect Immun 29:623-632.[Abstract/Free Full Text]
- Izutsu KT, Schubert MM, Truelove EL, Johnson DE (1987). Use of human minor salivary glands in basic and applied secretion research. J Dent Res 66:654-659.
- Kilian M., Nyvad B. (1990). Ability to bind salivary a-amylase discriminates certain viridans group streptococcal species. J Clin Microbiol 28:2576-2577.[Abstract/Free Full Text]
- Kilian M., Mikkelsen L., Henrichsen J. (1989). Taxonomic study of viridans streptococci: description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrewes and Horder 1906). Int J System Bacteriol 39:471-484.[Abstract/Free Full Text]
- Laible NJ, Germaine GR (1982). Adsorption of lysozyme from human whole saliva by Streptococcus sanguis 903 and other oral microorganisms. Infect Immun 36:148-159.[Abstract/Free Full Text]
- Laible NJ, Germaine GR (1985). Bactericidal activity of human lysozyme, muramidase-inactive lysozyme, and cationic polypeptides against Streptococcus sanguis and Streptococcus faecalis: inhibition by chitin oligosaccharides. Infect Immun 48:720-728.[Abstract/Free Full Text]
- Lamont RJ, Rosan B., Baker CT, Nelson GM (1988). Characterization of an adhesion antigen of Streptococcus sanguis G9B. Infect Immun 59:2417-2423.
- Lecomte P., Dawes C. (1987). The influence of salivary flow rate on diffusion of potassium chloride from artificial plaque at different sites in the mouth. J Dent Res 66:1614-1618.
- Liljemark WF, Bloomquist CG, Coulter MC, Fenner LJ, Skopek RJ, Schachtele CF (1988). Utilization of a continuous streptococcal surface to measure interbacterial adherence in vitro and in vivo. J Dent Res 67:1455-1460.
- Liljemark WF, Bloomquist C., Ofstenhage J. (1979). Aggregation and adherence of Streptococcus sanguis: role of human salivary immunoglobulin A. Infect Immun 26:1104-1110.[Abstract/Free Full Text]
- Lowry OH, Rosebrough N., Farr A., Randall R. (1951). Protein measurement with the folin phenol reagent. J Biol Chem 193:265-275.[Free Full Text]
- Malamud D. (1985). Influence of salivary proteins on the fate of oral bacteria. In: Molecular basis of oral microbial adhesion. Mergenhagen S, Rosan B, editors. Washington, DC: American Society for Microbiology, pp. 117-124.
- Pruitt KM, Reiter B. (1985). Biochemistry of peroxidase system: antimicrobial effects. In: The lactoperoxidase system. Pruitt K, Tenovuo J, editors. New York: Marcel Dekker, pp. 143-178.
- Rudney JD, Larson CJ (1994). Use of restriction fragment polymorphism analysis of rRNA genes to assign species to unknown clinical isolates of oral viridans streptococci. J Clin Microbiol 32:437-443.[Abstract/Free Full Text]
- Rudney JD, Kajander KC, Smith QT (1985). Correlations between human salivary levels of lysozyme, lactoferrin
- salivary peroxidase and secretory immunoglobulin A with different stimulatory states and over time. Arch Oral Biol 30:765-771.
- Rudney JD, Krig MA, Bloomquist CG, Liljemark WF (1991). Lactoferrin binding to bacterial monolayers on enamel chips in vitro (abstract). J Dent Res 70:497.
- Rudney JD, Krig MA, Neuvar EK (1993). Longitudinal study of relations between human salivary antimicrobial proteins and measures of dental plaque accumulation and composition. Arch Oral Biol 38:377-386.[CrossRef][Medline]
[Order article via Infotrieve]
- Rudney JD, Krig MA, Neuvar EK, Soberay AH, Iverson L. (1991). Antimicrobial proteins in human unstimulated whole saliva in relation to each other, and to measures of health status, dental plaque accumulation and composition. Arch Oral Biol 36:497-506.[CrossRef][Medline]
[Order article via Infotrieve]
- Rundegren J., Arnold R. (1987). Bacteria-agglutinating characteristics of secretory IgA and a salivary agglutinin. Adv Exp Med Biol 216:1005-1013.
- Scannapieco FA, Bhandary K., Ramasubbu N., Levine MJ (1990). Structural relationship between the enzymatic and streptococcal binding sites of human salivary a-amylase. Biochem Biophys Res Commun 173:1109-1115.[CrossRef][Medline]
[Order article via Infotrieve]
- Scannapieco FA, Haraszthy GG, Cho M-I., Levine MJ (1992). Characterization of an amylase-binding component of
- Streptococcus gordonii G9B. Infect Immun 60:4726-4733. Shern RJ, Fox PC, Cain JL, Li S-H. (1990). A method for measuring the flow of saliva from the minor salivary glands. J Dent Res 69:1146-1149.
- Shrout PE, Fleiss JL (1979). Intraclass correlations: uses in assessing rater reliability. Psychol Bull 86:420-428. Skopek RJ, Liljemark WF, Bloomquist CG, Rudney JD (1993). Dental plaque development on defined streptococcal surfaces. Oral Microbiol Immunol 8:16-23.[Medline]
[Order article via Infotrieve]
- Smith DJ, Taubman MA, Ali-Salaam P. (1991). Immunoglobulin isotypes in human minor gland saliva. J Dent Res 70:167-170. Sokal RR, Rohlf FJ (1981). Biometry. 2nd ed. San Francisco: Freeman.
- Stuchell RN, Mandel ID (1983). A comparative study of salivary lysozyme in caries-resistant and caries-susceptible adults. J Dent Res 62:552-554.
- Tenovuo J., Grahn E., Lehtonen O-P., Hyyppa T., Karhuvaara L., Vilja P. (1987). Antimicrobial factors in saliva: ontogeny and relation to oral health. J Dent Res 66:475-479.
- Van der Hoeven JS, Camp PJM (1991). Synergistic degradation of mucin by Streptococcus oralis and Streptococcus sanguis in mixed chemostat cultures. J Dent Res 70:1041-1044. Winer BJ (1971). Statistical principles in experimental design. 2nd ed. New York: McGraw-Hill.
Journal of Dental Research, Vol. 74, No. 6,
1280-1288 (1995)
DOI: 10.1177/00220345950740060701
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