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Quantifying the Strength of Bacterial Adhesive Interactions with Salivary Glycoproteins

Departments of Stomatologyl, Laboratory of Radiobiology and Environmental Health, University of California, San Francisco, California 94143-0512

C.A. Burdsal

Department of Anatomy, University of California, San Francisco, California 94143-0512

S.J. Fisher

Departments of Stomatologyl, Laboratory of Radiobiology and Environmental Health, University of California, San Francisco, California 94143-0512, Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143-0512, Department of Obstetrics, University of California, San Francisco, California 94143-0512, Department of Gynecology and Reproductive Sciences, University of California, San Francisco, California 94143-0512

We adapted an assay that has been used to estimate the strength of eukaryotic cell-cell and cell-extracellular matrix adhesive interactions (McClay et al., 1981) to quantify the strength of bacterial (streptococci, fusobacteria, actinomyces) interactions with salivary receptors. Bacteria are centrifuged onto human submandibular/sublingual or parotid-saliva-coated microtiter wells. Plates are sealed with pressure-sensitive, double-sided tape which allows them to be inverted and centrifuged again. The force required to remove the bacteria from the coated wells is a direct measure of the adhesive strength of the interaction(s) being disrupted. The bacteria-saliva adhesive forces we detected ranged from 1.6 x 10-8 dynes (Streptococcus sanguis 72-40) to > 1.1 x 10-7 dynes (Actinomyces viscosus T14 V). These forces were in the range to withstand the shear stress produced by salivary flow, which we calculated as approximately 6.1 x 10-7 dynes.

Key Words: bacteria • saliva • adhesion • quantifying

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Journal of Dental Research, Vol. 74, No. 5, 1212-1218 (1995)
DOI: 10.1177/00220345950740051101


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