This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kinney, J.H. Articles by Marshall, G.W. Search for Related Content PubMed PubMed Citation Articles by Kinney, J.H. Articles by Marshall, G.W., Jr Social Bookmarking                         What's this?

Mineral Distribution and Dimensional Changes in Human Dentin during Demineralization

L-356, PO Box 808, Chemistry and Materials Science Department, Lawrence Livermore National Laboratory, Livermore, California 94551

M. Balooch

L-356, PO Box 808, Chemistry and Materials Science Department, Lawrence Livermore National Laboratory, Livermore, California 94551

D.L. Haupt, Jr

L-356, PO Box 808, Chemistry and Materials Science Department, Lawrence Livermore National Laboratory, Livermore, California 94551

S.J. Marshall

University of California, San Francisco

G.W. Marshall, Jr

University of California, San Francisco

Many bonding agents require the dentin surface to be acid-etched prior to being bonded. Understanding the stability and morphology of the etched dentin surface is important for improving bond strength and reliability in these systems. In this study, the atomic force microscope was used to quantify dimensional changes that occur to fully hydrated dentin during demineralization with a pH 4.0 lactic acid gel. A high-resolution microtomography instrument, the x-ray tomographic microscope, was also used to quantify the mineral density distribution in the dentin as a function of etching time. The intertubular dentin surface shrank by less than 0.5 µm during etching, while the peritubular dentin receded at an initially rapid linear rate. The dentin surface retained its initial morphology, although it was more porous with the removal of the peritubular dentin. Beneath the etched surface, there were three major zones characterized by mineral density differences. The first zone was a fully demineralized collagen layer, subjacent to which was a partially demineralized zone of roughly constant mineral density. Immediately following the partially mineralized layer was normal dentin. The presence of the partially mineralized layer could be explained in terms of different transport rates in the peritubular and intertubular dentin.

Key Words: dentin • demineralization • shrinkage • atomic force microscopy • microtomography

REFERENCES

• Bertolotti RL (1992). Conditioning of the dentin substrate. Oper Dent Suppl 5:131-136.
• Eick JD ( 1992). Smear layer-materials surface. Proc Finn Dent Soc 88(Suppl 1):225-242.
• Eick JD, Robinson SJ, Byerley TJ, Chappelow CC (1993). Adhesives and nonshrinking dental resins of the future. Quintessence Int 24:632-640.[Medline] [Order article via Infotrieve]
• Fosse G., Saele PK, Eibe R. ( 1992). Numerical density and distributional pattern of dentine tubules. Acta Odontol Scand 50:201-210.[Medline] [Order article via Infotrieve]
• Garberoglio R., Brannstrom M. (1976). Scanning electron microscopic investigation of human dentinal tubules. Arch Oral Biol 21:355-362.[Medline] [Order article via Infotrieve]
• Kinney JH, Nichols MC (1992). X-ray tomographic microscopy using synchrotron radiation. Ann Rev Mater Sci 22:121-152.
• Kinney JH, Balooch M., Marshall GW, Marshall SJ (1993). Atomic force microscope study of dimensional changes in dentine during drying. Arch Oral Biol 38:1003-1007.[Medline] [Order article via Infotrieve]
• Kinney JH, Marshall GW, Marshall SJ (1994a). Three dimensional mapping of mineral densities in carious dentin: Theory and method. Scan Microsc 8: 197-205.
• Kinney JH, Haupt DL, Nichols MC, Breunig TM, Marshall GW, Marshall SJ (1994b). The x-ray tomographic microscope: Three-dimensional perspectives of evolving microstructures. Nucl Inst Meth A347:480-486.
• Marshall GW, Balooch M., Tench R., Kinney JH, Marshall SJ (1993). Atomic force microscopy of acid effects on dentin. Dent Mater 9:265-268.[Medline] [Order article via Infotrieve]
• Nakabayashi N. (1992). Adhesive bonding with 4-META. Oper Dent (Suppl 5):125-130.
• Nakabayashi N., Kojima K., Masuhara E. (1982). The promotion of adhesion by the infiltration of monomers into tooth substrates. J Biomed Mater Res 16:265-273.[CrossRef][Medline] [Order article via Infotrieve]
• Pashley DH, Ciucchi B., Sano H., Homer JA (1993). Permeability of dentin to adhesive agents. Quintessence Int 24:618-631. Ruben J., Arends J. (1993). Shrinkage of sound and demineralized human coronal dentine slabs. Caries Res 27:266-270.[Medline] [Order article via Infotrieve]
• ten Cate JM, Nyvad B., van de Plassche-Simons YM, Fejerskov O. (1991). A quantitative analysis of mineral loss and shrinkage of in vitro demineralized human root surfaces. J Dent Res 70:1371-1374.
• Van der Graaf ER, ten Bosch JJ (1993). Changes in dimensions and weight of human dentine after different drying procedures and during subsequent rehydration. Arch Oral Biol 38:97-99.[Medline] [Order article via Infotrieve]
• van Dijk JWE, Borggreven JMPM, Driessens FCM (1979). Chemical and mathematical simulation of caries. Caries Res 13:169-180.[Medline] [Order article via Infotrieve]
• Van Meerbeek B., Dhem A., Goret-Nicaise M., Braem MJA, Lambrechts P., Vanherle G. (1993). Comparative SEM and TEM examination of the ultrastructure of the resin-dentin interdiffusion zone. J Dent Res 72:495-501.
• Walshaw PS, McComb D. (1994). SEM evaluation of the resin-dentin interface with proprietary bonding agents in human subjects. J Dent Res 73:1079-1087.
• White JM, Goodis HE, Marshall SJ, Marshall GW (1994). Sterilization of teeth by gamma radiation. J Dent Res 73:1560-1567.

Journal of Dental Research, Vol. 74, No. 5, 1179-1184 (1995)
DOI: 10.1177/00220345950740050601


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J.I. Rosales, G.W. Marshall, S.J. Marshall, L.G. Watanabe, M. Toledano, M.A. Cabrerizo, and R. Osorio Acid-etching and Hydration Influence on Dentin Roughness and Wettability Journal of Dental Research, September 1, 1999; 78(9): 1554 - 1559. [Abstract] [PDF]

				T. Pioch, S. Stotz, H.J. Staehle, and H. Duschner Applications of Confocal Laser Scanning Microscopy to Dental Bonding Advances in Dental Research, November 1, 1997; 11(4): 453 - 461. [Abstract] [PDF]

				D.G. Gillam, N.J. Mordan, and H.N. Newman The Dentin Disc Surface: A Plausible Model for Dentin Physiology and Dentin Sensitivity Evaluation Advances in Dental Research, November 1, 1997; 11(4): 487 - 501. [Abstract] [PDF]

				J.H. Kinney, D.L. Haupt, M. Balooch, J.M. White, W.L. Bell, S.J. Marshall, and G.W. Marshall Jr The Threshold Effects of Nd and Ho:YAG Laser-induced Surface Modification on Demineralization of Dentin Surfaces Journal of Dental Research, June 1, 1996; 75(6): 1388 - 1395. [Abstract] [PDF]

				D.H. Pashley Dynamics of the Pulpo-Dentin Complex Critical Reviews in Oral Biology & Medicine, January 1, 1996; 7(2): 104 - 133. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kinney, J.H. Articles by Marshall, G.W. Search for Related Content PubMed PubMed Citation Articles by Kinney, J.H. Articles by Marshall, G.W., Jr Social Bookmarking                         What's this?