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Bovine Enamel Organ Cells Express Tissue Non-specific Alkaline Phosphatase mRNA

Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, 4001 Spruce Street, Philadelphia, PA 19104

E.E. Golub

Department of Biochemistry, University of Pennsylvania School of Dental Medicine, 4001 Spruce Street, Philadelphia, PA 19104

P.M. Collier

Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, 4001 Spruce Street, Philadelphia, PA 19104

C.W. Gibson

Department of Anatomy and Histology, University of Pennsylvania School of Dental Medicine, 4001 Spruce Street, Philadelphia, PA 19104

Alkaline phosphatase (AP) is expressed at high levels in all mineralizing tissues, and the isoform identified in developing enamel has biochemical properties similar to that found in bone. While the bone AP is referred to as the liver/bone/kidney or tissue non-specific (TNS) form, other APs are highly specific for tissue of expression. To determine unequivocally the AP isoform made by enamel organ cells, we constructed a fetal bovine enamel organ cDNA library, which yielded eight AP cDNA clones. In each case, the DNA sequence was homologous to the partial cDNA reported for bovine kidney AP (Garattini et al., 1987). It is concluded that enamel organ cells express the TNS-AP isoform. The extended 3' untranslated region of the cDNA has considerable homology to human TNS-AP, and the conservation of sequence suggests that the 3' end may have a role in post-transcriptional regulation of expression.

Key Words: alkaline phosphatase • enamel organ • cDNA • bovine

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Journal of Dental Research, Vol. 74, No. 12, 1886-1890 (1995)
DOI: 10.1177/00220345950740121201


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