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Alternative Splicing of Amelogenin mRNA from Rat Incisor Ameloblasts

Department of Pathology, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14620

W. Li

Department of Growth and Development, Box 0438, University of California at San Francisco, San Francisco, CA 94143-0438

P.K. DenBesten

Department of Growth and Development, Box 0438, University of California at San Francisco, San Francisco, CA 94143-0438

Amelogenin proteins are a major component of the developing enamel matrix, and are likely to have a key role in the control of enamel biomineralization. The heterogeneity of amelogenin is in part due to alternative splicing of the amelogenin RNA transcripts. Several patterns of alternative splicing have been described in mouse, bovine, porcine, and human enamel, with all alternatively spliced products having homologous 5' and 3' sequences within the coding regions. In these studies, we have used anchored PCR to identify alternatively spliced amelogenin cDNA sequences in the rat. We found amelogenin cDNAs that could be divided into two groups based on their 3' sequence. Group 1 cDNAs had a novel terminal sequence that has not been previously identified, while group 2 cDNAs were similar to those previously identified in other animal species. We identified a sequence, identical to the novel 3' amelogenin cDNA sequence, in rat genomic DNA downstream from the previously identified exon 7. The putative amelogenin proteins predicted for the two groups differed in their predicted isoelectric points, with the novel group 1 proteins having a more basic isoelectric poi

Key Words: amelogenin • rat • alternative splicing • PCR • dental enamel

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Journal of Dental Research, Vol. 74, No. 12, 1880-1885 (1995)
DOI: 10.1177/00220345950740121101


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Li, R. Articles by DenBesten, P.K. Search for Related Content PubMed PubMed Citation Articles by Li, R. Articles by DenBesten, P.K. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Substance via MeSH Social Bookmarking                         What's this?