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Effect of Maturation on 31P Magnetic Resonance Spectroscopy of the Rabbit Masseter Muscle

Department of Growth and Development, University of California, 900 Irving St., San Francisco, CA 94143

W. Chew

School of Dentistry, and Magnetic Resonance Research Center, School of Medicine, University of California, 900 Irving St., San Francisco, CA 94143

A.J. Decrespigny

School of Dentistry, and Magnetic Resonance Research Center, School of Medicine, University of California, 900 Irving St., San Francisco, CA 94143

M. Alcantara

Department of Growth and Development, University of California, 900 Irving St., San Francisco, CA 94143

C. McNeill

Center for Temporomandibular Disorders and Orofacial Pain, University of California, 900 Irving St., San Francisco, CA 94143

A.J. Miller

Department of Growth and Development, University of California, 900 Irving St., San Francisco, CA 94143, Center for Temporomandibular Disorders and Orofacial Pain, University of California, 900 Irving St., San Francisco, CA 94143

This work studies the dynamic metabolic changes of the rabbit masseter muscle during post-natal development. The composition and proportion of oxidative and glycolytic muscle fibers alter during maturation. The masseter muscle, as most muscles of the craniofacial region, exhibits unusual development in composition of isoforms of myosin. The effect of this unusual composition on the dynamic metabolic properties of the masseter muscle have not been assessed. The metabolism of the rabbit masseter muscle was studied by means of 31P-nuclear magnetic resonance (NMR) spectroscopy. Contraction was elicited by electrical stimulation of the muscle in the anesthetized animal. Five animals were studied at 8 weeks and 24 weeks so that both the juvenile and adult stages could be evaluated. The dynamic biochemical changes in the masseter muscle were studied by the analysis of NMR spectra. A single-turn surface coil (copper) was used, and the original signal was treated with Fourier transforms to obtain ³¹P spectra. The low signal-to-noise ratio required averaging 16 acquisitions (acquisition time = 400 msec, repetition rate = 1.8 sec) in 30 sec and then obtaining continuous spectra for 27 min. Each averaged spectrum demonstrated five peaks: inorganic phosphate (Pi), creatine phosphate (PCr), and three peaks related to adenosine triphosphate (ATP). The protocol involved recording an initial three-minute rest period, stimulating the muscle at 5 Hz for 3 min twice, separated by three-minute rest periods, and stimulating the muscle at 50 Hz twice for 3 min separated by rest periods. The Pi/PCr ratio increased significantly in the adult masseter during both 5-Hz stimulations, evoking twitching, and the first 50-Hz stimulation, evoking tetany (repeated ANOVA, P < 0.05). The resting pH (6.96 ± 0.13) was significantly lowered during both twitching (6.85 ± 0.10; P < 0.0038) and tetany (6.55 ± 0.13; P < 0.0001), but only in the adult masseter muscle. These findings suggest that the adult masseter muscle possesses more glycolytic fibers as it modifies its metabolism during postnatal development.

Key Words: Nuclear magnetic resonance • muscle contraction • masseter muscle • rabbits

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Journal of Dental Research, Vol. 74, No. 12, 1861-1869 (1995)
DOI: 10.1177/00220345950740120901


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Chang, C. Articles by Miller, A.J. Search for Related Content PubMed PubMed Citation Articles by Chang, C. Articles by Miller, A.J. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Social Bookmarking                         What's this?