|
Sign In to gain access to subscriptions and/or personal tools.
|
Mineral Element Analysis of Carious and Sound Rat Dentin by Electron Probe Microanalyzer Combined with Back-scattered Electron Image
L. Tjäderhane
Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland
E.-L. Hietala
Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland
M. Larmas
Institute of Dentistry, University of Oulu, Aapistie 3, 90220 Oulu, Finland, Oulu University Hospital
We recently demonstrated the advantages of back-scattered electron images (COMPO) in the visualization of dentinal caries, and the relationship of the change in the dentin fluorescence pattern in caries lesions. However, the exact nature of these changes is not known. In this paper, the nature of the changes in the areas with reduced mineral content in COMPO images was investigated. We examined the relation of changes in mineral elements and the appearance of soft carious and sound dentin in COMPO images using a scanning electron microscope (SEM) equipped with an electron probe microanalyzer (EPMA). Rat molars with small dentinal caries lesions just under the DEJ were chosen for the study. The Ca, P, Na, Mg, Zn, F, and total contents were determined by EPMA from five different dentin sites, and the Ca/P and Mg/Ca ratios were calculated. Generally, the lowest contents were found in caries lesions and highest in mantle dentin, with the exceptions of Mg and Zn. The Ca/P ratio was lowest in mantle dentin and highest in carious dentin. The results confirm that the change in fluorescence in the dentinal caries lesion is correlated with the very initial changes in mineral content, and that EPMA used in combination with COMPO images is a useful tool for determining small changes in mineral elements in the carious and adjacent areas of dentin.
Key Words: dentin • rat • normal • carious • mineral elements
REFERENCES
- Birkedal-Hansen H., Moore WGI, Bodden MK, Windsor LJ, Birkedal-Hansen B., DeCarlo A., et al. (1993). Matrix
- metalloproteinases: a review. Crit Rev Oral Biol Med 4:197-250. Chickerur NS, Tung MS, Brown WE (1980). A mechanism for incorporation of carbonate into apatite. Calcif Tissue Int 32:55-62.[CrossRef][Medline]
[Order article via Infotrieve]
- Daculsi G., LeGeros RZ, Jean A., Kerebel B. (1987). Possible physico-chemical processes in human dentin caries. J Dent Res 66:1356-1359.
- Driessens FCM (1980). Probable phase composition of the mineral in bone. Z Natürforsch 35(c):357-362.
- Driessens FCM, Verbeeck RMH (1982). The probable phase composition of the mineral in sound enamel and dentine. Bull Soc Chim Belg 91:573-596.
- Driessens FCM, van Dijk JWE, Borggreven Jmpm (1978). Biological calcium phosphates and their role in the physiology of bone and dental tissues. I. Composition and solubility of calcium phosphates. Calcif Tissue Res 26:127-137.
- Frank RM, Capitant M., Goni J. ( 1966). Electron probe studies of human enamel. J Dent Res 45(Suppl):672-682.
- Hals E., Tveit AB, Totdal B. (1988). X-ray microanalysis of dentin: a review. Scanning Microsc 2:357-369.[Medline]
[Order article via Infotrieve]
- Halse A. (1971). Electron microprobe analysis of deep layers of rat incisor enamel. Scand J Dent Res 79:377-380.[Medline]
[Order article via Infotrieve]
- Hietala E-L., Tjaderhane L., Larmas M. (1993). Dentin caries recording with Schiff's reagent, fluorescence, and back-scattered electron image. J Dent Res 72:1588-1592.
- Jenkins GN (1978). Chemical composition of teeth. In: The physiology and biochemistry of the mouth. 4th ed. Jenkins GN, editor. Oxford: Blackwell Scientific Publications, pp. 56-112.
- Kobayashi Y., Ozeki M., Ogawa A., Matsumoto S., Sanjo M., Moriyama T. (1992). Invasion of Streptococcus mutans, Streptococcus intermedius and Propionibacterium acnes into the teeth of gnotobiotic rats. Caries Res 26:132-138.[Medline]
[Order article via Infotrieve]
- Larmas M., Kortelainen S. (1989). Quantification of the areas of dentinal lesions and secondary dentin in fissures of rat molars. Caries Res 23:32-35.[Medline]
[Order article via Infotrieve]
- Larsen MJ, Bruun C. (1986). Enamel/saliva-inorganic chemical reactions. In: Textbook of cariology. Thylstrup A, Fejerskov 0, editors. Copenhagen: Munksgaard, pp. 181-203.
- LeGeros RZ (1990). Chemical and crystallographic events in the caries process. J Dent Res 69:567-574.
- Levine RS (1971). The distribution of hydroxyproline in sound human coronal dentine. Arch Oral Biol 16:473-478.[Medline]
[Order article via Infotrieve]
- Lormee P., Weill R., Septier D. (1986). Morphological and histochemical aspects of carious dentine in Osborne-Mendel rats. Caries Res 20:251-262.[Medline]
[Order article via Infotrieve]
- Morgan AJ (1985). X-ray microanalysis in electron microscopy for biologists. Oxford: Oxford University Press.
- Potter RH (1994). Significance level and confidence interval (guest editorial). J Dent Res 73:494-496.
Journal of Dental Research, Vol. 74, No. 11,
1770-1774 (1995)
DOI: 10.1177/00220345950740110901
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Valikangas, E. Pekkala, M. Larmas, J. Risteli, T. Salo, and L. Tjaderhane
The Effects of High levels of Glucose and Insulin on Type I Collagen Synthesis in Mature Human Odontoblasts and Pulp Tissue in vitro
Advances in Dental Research,
August 1, 2001;
15(1):
72 - 75.
[Abstract]
[PDF]
|
|
|
|
|
