|
Sign In to gain access to subscriptions and/or personal tools.
|
Binding of Human High-molecular-weight Salivary Mucins (MG1) to Hemophilus parainfluenzae
E.C.I. Veerman
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, Van der Boechorststraat 7,1081 BT Amsterdam, The Netherlands
A.J.M. Ligtenberg
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, Van der Boechorststraat 7,1081 BT Amsterdam, The Netherlands
L.C.P.M. Schenkels
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, Van der Boechorststraat 7,1081 BT Amsterdam, The Netherlands
E. Walgreen-Weterings
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, Van der Boechorststraat 7,1081 BT Amsterdam, The Netherlands
A.V. Nieuw Amerongen
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, Van der Boechorststraat 7,1081 BT Amsterdam, The Netherlands
In human saliva, two different mucin populations can be distinguished, viz., high-molecular-weight mucins (MG1, mol. wt > 1 x 106) and low-molecular-weight mucins (MG2, mol. wt -125 kD). The carbohydrate moiety of MG1 displays a wide spectrum of oligosaccharide structures, varying in composition, length, branching, and acidity. The biological significance of the heterogeneity in carbohydrate structures of mucins is unclear. The present investigation focused on the question whether MG1, because of its diverse carbohydrate side-chain population, can bind to a large variety of oral micro-organisms. A replica plate technique, in combination with immunochemical detection with monoclonal antibodies against MG1, was used to screen in vivo human oral microflora for the presence of micro-organisms which could bind the high-molecular-weight salivary mucin MG1. Binding to purified MG1 was established for Hemophilus (para)influenzae species, whereas other species, including Streptococcus and Staphylococcus, were negative. MG1 binding to Hemophilus parainfluenzae could be abolished by protease treatment of MG1. In contrast, periodate acid treatment, partial deglycosylation, or addition of monosaccharides did not affect MG1 binding to H. parainfluenzae, indicating that MG1 carbohydrate side-chains were not directly involved in the binding. The binding was pH-dependent, showing an increase when the pH was lowered from 8.0 to 4.0. These data indicate that MG1 can be bound in a selective manner by Hemophilus spp. and suggest that the 'naked' unglycosylated polypeptide moiety of MG1 is involved in its binding to Hemophilus parainfluenzae.
Key Words: saliva • mucins • MG1 • hemophilus • binding.
REFERENCES
- Biesbrock AR, Reddy MS, Levine MJ (1991). Interaction of a salivary mucin-secretory immunoglobulin A complex with mucosal pathogens. Infect Immun 59:3492-3497.[Abstract/Free Full Text]
- Bradway SD, Bergey EJ, Scannapieco FA, Ramasubbu N., Sawacki S., Levine MJ (1992). Formation of salivary-mucosal pellicle: the role of transglutaminase. Biochem J 284:557-564.[Medline]
[Order article via Infotrieve]
- Braun W., Abraham R. (1989). Modified diffusion blotting for rapid and efficient protein transfer with PhastSystem. Electrophoresis 10:249-253.[Medline]
[Order article via Infotrieve]
- Ellen RP (1985). Specificity of attachment as a tissue-tropic influence on oral bacteria. In: Molecular basis of oral microbial adhesion. Mergenhagen P, Rosan B, editors. Washington, DC: American Society for Microbiology, pp. 33-40.
- Embery G., Heany TG, Stansbury JB (1986). Studies on the organic polyanionic constituents of human acquired dental pellicles. Arch Oral Biol 31:623-625.[CrossRef][Medline]
[Order article via Infotrieve]
- Hoffman MP, Haidaris C. (1993). Analysis of Candida albicans adhesion to salivary mucin. Infect Immun 61:1940-1949.[Abstract/Free Full Text]
- Kilian M., Schiott CR (1975). Haemophili and related bacteria in the human oral cavity. Arch Oral Biol 20:791-796.[CrossRef][Medline]
[Order article via Infotrieve]
- Kilian M., Prachyabrued W., Theilade E. (1976). Haemophili in developing dental plaque. Scand J Dent Res 84:16-91.[Medline]
[Order article via Infotrieve]
- Klein A., Carnoy CO, Wieruszeski J-M., Strecker G., Strang A.-M, van Halbeek H., et al. (1992). The broad diversity of neutral and sialylated oligosaccharides derived from human salivary mucins. Biochemistry 31:6152-6165.
- Kolenbrander PE (1993). Coaggregation of human oral bacteria: potential role in the accretion of dental plaque. J Appl Bacteriol 74: 79S-86S.[CrossRef]
- Lamblin G., Lhermitte M., Klein A., Houdret N., Scharfman A., Ramphal R., et al. (1991). The carbohydrate diversity of human respiratory mucins: a protection of the underlying mucosa? Am Rev Respir Dis 144:S19-S24.[Medline]
[Order article via Infotrieve]
- Ligtenberg AJM, Walgreen-Weterings E., Veerman ECI, de Soet JJ, de Graaff J., Nieuw Amerongen AV (1992). Influence of saliva on aggregation and adherence of Streptococcus gordonii HG222. Infect Immun 60:3878-3884.
- Liljemark WF, Bandt CL, Bloomquist CG, Uhl L. (1984). The distribution of oral Haemophilus species in dental plaque from a large adult population. Infect Immun 46:778-786.[Abstract/Free Full Text]
- Liljemark WF, Bloomquist CG, Fenner LJ (1985). Characteristics of the adherence of oral Haemophilus species to an experimental salivary pellicle and to other bacteria. In: Molecular basis of oral microbial adhesion. Mergenhagen SE, Rosan B, editors. Washington, DC: American Society for Microbiology, pp. 94-103.
- Liljemark WF, Fenner LJ, Bloomquist CG (1986). In vivo colonization of salivary pellicle by Haemophilus, Actinomyces and Streptococcus species. Caries Res 20:481-497.[Medline]
[Order article via Infotrieve]
- Loomis RE, Prakobphol A., Levine MJ, Reddy MS, Jones PC (1987). Biochemical and biophysical comparison of two mucins from human submandibular-sublingual saliva. Arch Biochem Biophys 258:452-464.[CrossRef][Medline]
[Order article via Infotrieve]
- Murray PA, Prakobphol A., Lee T., Hoover CI, Fisher SJ (1992). Adherence of oral streptococci to salivary glycoproteins. Infect Immun 60:31-38.[Abstract/Free Full Text]
- Murray PA, Levine MJ, Tabak LA, Reddy MS (1982). Specificity of salivary-bacterial interactions. II. Evidence for a lectin on Streptococcus sanguis with specificity for a NeuAc
 2,3Galß1,3GalNAc sequence. Biochem Biophys Res Commun 106:390-396.[CrossRef][Medline]
[Order article via Infotrieve] - Prakobphol A., Levine MJ, Tabak LA, Reddy MS (1982). Purification of a low-molecular weight, mucin type glycoprotein from human submandibular-sublingual saliva. Carbohydr Res 108:111-122.[CrossRef][Medline]
[Order article via Infotrieve]
- Ramasubbu N., Reddy MS, Bergey EJ, Haraszthy GG, Soni S.-D, Levine MJ (1992). Large scale purification and characterization of the major phosphoproteins and mucins of human submandibular-sublingual saliva. Biochem J 280:341-352.
- Rathman WM, van den Keijbus PAM, van Zeyl MJ, Dopp EA, Veerman ECI, Nieuw Amerongen AV (1990). Characterization of monoclonal antibodies to human salivary (glyco)proteins. Cellular localization of mucin, cystatin-like 14 kD protein and 20 kD glycoprotein in the human submandibular gland. J Biol Buccale 18:19-27.[Medline]
[Order article via Infotrieve]
- Schenkels Lcpm, Ligtenberg AJM, Veerman ECI, Nieuw Amerongen AV (1993). Interaction of the salivary glycoprotein EP-GP with the bacterium Streptococcus salivarius HB. J Dent Res 72:1559-1565.
- Slots J., Taubman MA (1992). Contemporary oral microbiology and immunology. Reinhardt RW, editor. St. Louis, MO: Mosby-Year Book, p. 324.
- Stinson MW, Levine MJ, Cavese JM, Prakobphol A., Murray PA, Tabak LA, et al. (1982). Adherence of Streptococcus sanguis to salivary mucin bound to glass. J Dent Res 61:1390-1393.
- Tseng CC, Scannapieco FA, Levine MJ (1992). Use of a replica-plate assay for the rapid assessment of salivary protein-bacteria interactions. Oral Microb Immunol 7:53-56.[Medline]
[Order article via Infotrieve]
- Veerman ECI, Valentijn-Benz M., Bank R., Nieuw Amerongen AV (1989). Isolation of high molecular weight mucins from human whole saliva by ultracentrifugation. J Biol Buccale 17:302-312.
- Veerman ECI, Valentijn-Benz M., van den Keijbus PAM, Rathman WM, Sheehan JK, Nieuw Amerongen AV (1991). Immunochemical analysis of salivary mucins using monoclonal antibodies. Arch Oral Biol 34:923-932.
- Veerman ECI, van den Keijbus PAM, Valentijn-Benz M., Nieuw Amerongen AV (1992). Isolation of different high molecular weight mucin species from human whole saliva. Biochem J 283:807-811.[Medline]
[Order article via Infotrieve]
Journal of Dental Research, Vol. 74, No. 1,
351-357 (1995)
DOI: 10.1177/00220345950740011101
 CiteULike  Complore  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter What's this?
This article has been cited by other articles:
|
|
|
|
 
J. A. Bosch, M. Turkenburg, K. Nazmi, E. C. I. Veerman, E. J. C. de Geus, and A. V. Nieuw Amerongen
Stress as a Determinant of Saliva-Mediated Adherence and Coadherence of Oral and Nonoral Microorganisms
Psychosom Med,
July 1, 2003;
65(4):
604 - 612.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E.C.I. Veerman, P.A.M. van den Keijbus, K. Nazmi, W. Vos, J.E. van der Wal, E. Bloemena, J.G.M. Bolscher, and A.V. N. Amerongen
Distinct localization of MUC5B glycoforms in the human salivary glands
Glycobiology,
May 1, 2003;
13(5):
363 - 366.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Thomsson, A. Prakobphol, H. Leffler, M. S. Reddy, M. J. Levine, S. J. Fisher, and G. C. Hansson
The salivary mucin MG1 (MUC5B) carries a repertoire of unique oligosaccharides that is large and diverse
Glycobiology,
January 1, 2002;
12(1):
1 - 14.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Bosch, E. J. C. de Geus, T. J. M. Ligtenberg, K. Nazmi, E. C. I. Veerman, J. Hoogstraten, and A. V. N. Amerongen
Salivary MUC5B-Mediated Adherence (Ex Vivo) of Helicobacter pylori During Acute Stress
Psychosom Med,
January 1, 2000;
62(1):
40 - 49.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.D. Rudney, K.L. Hickey, and Z. Ji
Cumulative Correlations of Lysozyme, Lactoferrin, Peroxidase, S-IgA, Amylase, and Total Protein Concentrations with Adherence of Oral Viridans Streptococci to Microplates Coated with Human Saliva
Journal of Dental Research,
March 1, 1999;
78(3):
759 - 768.
[Abstract]
[PDF]
|
|
|
|
|
|
|
F. Namavar, M. Sparrius, E. C. I. Veerman, B. J. Appelmelk, and C. M. J. E. Vandenbroucke-Grauls
Neutrophil-Activating Protein Mediates Adhesion of Helicobacter pylori to Sulfated Carbohydrates on High-Molecular-Weight Salivary Mucin
Infect. Immun.,
February 1, 1998;
66(2):
444 - 447.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C.P.M. Schenkels, E. C.I. Veerman, and A. V. Nieuw Amerongen
Biochemical Composition of Human Saliva in Relation To Other Mucosal Fluids
Critical Reviews in Oral Biology & Medicine,
January 1, 1995;
6(2):
161 - 175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.D. Rudney
Does Variability in Salivary Protein Concentrations Influence Oral Microbial Ecology and Oral Health?
Critical Reviews in Oral Biology & Medicine,
January 1, 1995;
6(4):
343 - 367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
