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Journal of Dental Research
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Identification of Bone-type Alkaline Phosphatase mRNA from Human Periodontal Ligament Cells

M. Goseki

Department of Biochemistry

S. Oida

Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113

K. Takeda

First Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113

Y. Ogata

Department of Periodontology, Nihon University School of Dentistry at Matsudo, Sakaecho-Nishi 2-870-1, Matsudo, Chiba 271, Japan

T. Iimura

Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113

Y. Maruoka

Second Department of Oral and Maxillofacial Surgery, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113

S. Sasaki

Department of Biochemistry, School of Dentistry, Tokyo Medical and Dental University, 5-45, Yushima 1-Chome, Bunkyo-ku, Tokyo 113

Tissue-nonspecific-type alkaline phosphatase is found in the bone, liver, kidney, and other tissues, and its gene consists of 12 exons with the coding sequence beginning in the second exon. Recently, an alternative noncoding first exon was identified in the liver message which differed from that of the previously known osteoblast-derived cDNA sequence. Although these two mRNAs produce an identical protein, they have different promoter regions. The periodontal ligament tissue expresses a high level of alkaline phosphatase activity. To identify its mRNA type, we isolated a full-length cDNA for alkaline phosphatase from a cultured human periodontal ligament cell expression library, using bone-derived tissue-nonspecific alkaline phosphatase cDNA as a hybridization probe. The size of this clone was 2.5 kb, and its 5' and 3' untranslated sequences were identical to those of the human tissue-nonspecific type isolated from osteoblastic cells but not to those of the liver type. In addition, the same fragments as in bone-derived tissue-nonspecific-type cDNA were detected by the treatment of the cDNA clone with restriction enzymes Hinc II and Pst I. The results suggest that expression of the same alkaline phosphatase isozyme in human periodontal ligament cells may be regulated by the same transcriptional mechanism as in bone.

Key Words: alkaline phosphatase • periodontal ligament • gene expression • messenger RNA.

REFERENCES

  • Chomczynski P., Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 162:156-159.[Medline] [Order article via Infotrieve]
  • Denhardt DT (1966). A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641-646.[CrossRef][Medline] [Order article via Infotrieve]
  • Goseki M., Oida S., Nifuji A., Sasaki S. (1990). Properties of alkaline phosphatase of the human dental pulp. J Dent Res 69:909-912.
  • Henthom PS, Rasucha M., Kadesch T., Weiss MJ, Harris H. (1988). Sequence and characterization of the human intestinal alkaline phosphatase gene. J Biol Chem 263:12011-12019.[Abstract/Free Full Text]
  • Henthorn PS, Raducha M., Fedde KN, Lafferty MA, Whyte MP (1992). Different missense mutations at the tissue-nonspecific alkaline phosphatase gene locus in autosomal recessively inherited forms of mild and severe hypophosphatasia. Proc Natl Acad Sci USA 89:9924-9928.[Abstract/Free Full Text]
  • Kawase T., Sato S., Miake K., Saito S. (1988). Alkaline phosphatase of human periodontal ligament fibroblast-like cells. Adv Dent Res 2:234-239.[Abstract/Free Full Text]
  • Knoll BJ, Rothblum KN, Longley M. (1988). Nucleotide sequence of the human placental alkaline phosphatase gene. J Biol Chem 263:12020-12027.[Abstract/Free Full Text]
  • Matsuura S., Kishi F., Kajii T. (1990). Characterization of 5'flanking region of the liver/bone/kidney alkaline phosphatase gene: two kinds of mRNA from a single gene. Biochem Biophys Res Commun 168:993-1000.[CrossRef][Medline] [Order article via Infotrieve]
  • Ogata Y., Yokota Y., Niisato N., Furuyama S., Sugiya H. (1994). Presence of endogenous chemotactic factors for periodontal ligament cells in cementum and bone. Arch Oral Biol 39:529-533.[Medline] [Order article via Infotrieve]
  • Oida S., Goseki M., Sasaki S. (1985). Purification and subunit structure of alkaline phosphatase from bovine enamel organ. Arch Oral Biol 30:193-196.[Medline] [Order article via Infotrieve]
  • Sanger F., Nicklen S., Coulson AR (1977). DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467.[Abstract/Free Full Text]
  • Sato S., Kawase T., Kubota T., Saito S. (1990). Isolation and partial characterization of bovine periodontal ligament alkaline phosphatase. Bull Kanagawa Dent Coll 18:43-53.[Medline] [Order article via Infotrieve]
  • Somerman MJ, Archer SY, Imm GR, Foster RA (1988). A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 67:66-70.
  • Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C., Raducha M., Harris H. (1986). Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase. Proc Natl Acad Sci USA 83:7182-7186.[Abstract/Free Full Text]
  • Weiss MJ, Ray K., Henthorn PS, Lamb B., Kadesch T., Harris H. (1988a). Structure of the human liver/bone/kidney alkaline phosphatase gene. J Biol Chem 263:12002-12010.[Abstract/Free Full Text]
  • Weiss MJ, Cole DEC, Ray K., Whyte MP, Lafferty MA, Mulivor RA, et al. (1988b). A missense mutation in the human liver/bone/kidney alkaline phosphatase gene causing a lethal form of hypophosphatasia. Proc Natl Acad Sci USA 85:7666-7669.[Abstract/Free Full Text]
  • Whyte MP (1989). Hypophosphatasia. In: Bone and mineral research. Vol. 6. Peck WA, editor. New York: Elsevier Science Publishers, pp. 175-218.

Journal of Dental Research, Vol. 74, No. 1, 319-322 (1995)
DOI: 10.1177/00220345950740010601
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