Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

CiteULike is a free service for managing and discovering scholarly references - click here to get started.

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Nakashima, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakashima, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Induction of Dentin Formation on Canine Amputated Pulp by Recombinant Human Bone Morphogenetic Proteins (BMP)-2 and -4

M. Nakashima

Department of Conservative Dentistry, Faculty of Dentistry, Kyushu University, 61, Fukuoka 812, Japan

Dental pulp cells have the potential to differentiate into odontoblasts. The molecular mechanisms underlying differentiation are not clear. Demineralized dentin matrix is osteoinductive and contains bone morphogenetic protein (BMP) activity. BMPs have been implicated in embryonic odontogenic differentiation and hence may play a role in the differentiation of adult pulp cells into odontoblasts during pulpal healing. This study examined the hypothesis that BMPs induce dentin formation on amputated canine pulp. Recombinant human BMP-2 and BMP-4 were capped with inactivated dentin matrix on amputated pulp. At two months, the amputated pulp was filled with tubular dentin in the lower part and osteodentin in the upper part. The amount of dentin formed was markedly diminished when dentin matrix alone was implanted. These findings imply that recombinant human BMP-2 and BMP-4 induce differentiation of adult pulp cells into odontoblasts. Thus, BMPs may have a role in dentistry as a bioactive pulp-capping agent to induce dentin formation.

Key Words: Bone Morphogenetic Proteins-2 and -4 • Dentin • Dental Pulp • Odontoblasts • Differentiation • Dental Pulp Capping • Pulpotomy

REFERENCES

  • Baume LJ (1980). The biology of pulp and dentine. In: Monograph in oral science. Myers HM, editor. Basel: S. Karger, pp. 159-182.
  • Beque-Kirn C., Smith AJ, Ruch JV, Wozney JM Purchio A. Hartmann D., Lesot H. (1992). Effects of dentin proteins, transforming growth factor β1 (TGF-β) and bone morphogenecic protein 2 (BMP2) on the differentiation of odontoblast in vitro. Int J Dev Biol 36:491-503.[Medline] [Order article via Infotrieve]
  • Berman DS, Massler M. (1958). Experimental pulpotomies in rat molars. J Dent Res 37:229-242.
  • Bessho K., Tagawa T. Murata M. (1990), purification of rabbit bone morphogenetic protein derived from bone, dentin, and wound tissue after tooth extraction. J Oral Maxillofac Surg 48:162-169.[Medline] [Order article via Infotrieve]
  • Bessho K., Tanaka N., Matsumoto J., Tagawa T., Murata M. (1991). Human dentin-matrix-derived bone morphogenetic protein. J Dent Res 70:171-175.
  • Beust TB (1931). Physiologic changes in the dentin. J Dent Res 11:267-275.
  • Butler WT, Mikulski A., Urist MR (1977). Non-collagenous proteins of a rat dentin matrix possessing bone morphogenetic activity. J Dent Res 56:228-232.
  • Celeste AJ, lannazzi JA, Taylor RC, Hewick RM, Rosen V., Wang EA, Wozney JM (1990). Identification of trausforming growth factor β family members present in bone-inductive protein purified from bovine bone. Proc Natl Acad Sci USA 87:9843-9847.[Abstract/Free Full Text]
  • Conover MA, Urist MR (1982). Dentin matrix bone morphogenetic protein (abstract). In: Proceedings of the 1st International Conference on Chemistry and Biology of Mineralized Connective Tissue, Northwestern University. New York: Elsevier-North Holland, pp. 597-606.
  • Cox CF, Keall CL, Keall HJ, Ostro E., Bergenholtz G. (1987). Biocompatibility of surface-sealed dental materials against exposed pulps. J Prosthet Dent 57:1-8.[Medline] [Order article via Infotrieve]
  • Cox CF, White KC, Ramus DL, Farmer JB, Snuggs HM (1992). Reparative dentin: factors affecting its deposition. Quintessence Int 23:237-270.[Medline] [Order article via Infotrieve]
  • Fitzgerald M. (1979). Cellular mechanics of dentinal bridge repair using 3H-thymidine. J Dent Res 38:2198-2206.
  • Hammonds RG Jr, Schwall R., Dudley A., Berkemeier L., Lal C. Lee J., et al. (1991). Bone-inducing activity of mature BMP-2b produced from a hybrid BMP-2a/2b precursor. Mol Endocrinol 5:149-155.[Abstract/Free Full Text]
  • Katz RW, Reddi AH (1988). Dissociative extraction and partial purification of osteogenin, a bone inductive protein, from rat tooth matrix by heparin affinity chromatography. Biochem Biophys Res Commun 157:1233-1237.
  • Kawai T., Urist MR (1989). Bovine tooth-derived bone morphogenetic protein. J Dent Res 68:1069-1074.
  • Lyons KM, Pelton RW, Hogan BLM (1990). Organogenesis and pattern formation in the mouse: RNA distribution patterns suggest a role for bone morphogenetic protein-2A (BMP-2A). Development 109:833-844.[Abstract/Free Full Text]
  • Mera K. ( 1988). Studies on bone morphogenetic protein (BMP) derived from bovine demineralized dentin matrix. JJpn Stomatol Soc 37:389-399.
  • Muthukumaran N., Ma S., Reddi AH (1988). Dose-dependence of and threshold for optimal bone induction by collagenous bone matrix and osteogenin-enriched fraction. Collagen Rel Res 8:433-441.
  • Nakashima M. (1989). Dentin induction by implants of autolyzed antigen-extracted allogeneic (AAA) dentin on amputated pulps of dogs. Endod Dent Traumatol 5:279-286.[CrossRef][Medline] [Order article via Infotrieve]
  • Nakashima M. (1990a). An ultrastructural study of the differentiation of mesenchymal cells in implants of allogeneic dentine matrix on the amputated dental pulp of the dog. Arch Oral Biol 35:277-281.[Medline] [Order article via Infotrieve]
  • Nakashima M. (1990b). The induction of reparative dentine in the amputated dental pulp of the dog by bone morphogenetic protein. Arch Oral Biol 35:493-497.[Medline] [Order article via Infotrieve]
  • Nakashima M. (1992). The effects of growth factors on DNA synthesis, proteoglycan synthesis and alkaline phosphatase activity in bovine dental pulp cells. Arch Oral Biol 37:231-236.[CrossRef][Medline] [Order article via Infotrieve]
  • Nakashima M., Nagasawa H., Yamada Y., Reddi AH (1994). Regulatory role of transforming growth factor-β, bone morphogenetic protein-2, and protein-4 on gene expression of extracellular matrix proteins and differentiation of dental pulp cells. Dev Biol 162:18-28.[CrossRef][Medline] [Order article via Infotrieve]
  • Ozkaynak E., Rueger DC, Drier EA, Corbett C., Ridge RJ, Sampath TK, et al. (1990). OP-I cDNA encodes an osteogenic protein in the TGF-β family. EMBOJ 9:2085-2093.[Medline] [Order article via Infotrieve]
  • Ozkaynak E., Schnegelsberg PNJ, Jin DF, Clifford GM, Warren FD, Drier EA, et al. (1992). Osteogenic protein-2. A new member of the transforming growth factor-β superfamily expressed early in embryogenesis. J Biol Chem 267:25220-25227.[Abstract/Free Full Text]
  • Reddi AH (1992). Regulation of cartilage and bone differentiation by bone morphogenetic proteins. Curr Opin Cell Biol 4:850-855.[CrossRef][Medline] [Order article via Infotrieve]
  • Rizzino A. (1988). Transforming growth f actor-p: multiple effects on cell differentiation and extracellular matrices. Dev Biol 130:411-422.[CrossRef][Medline] [Order article via Infotrieve]
  • Ruch JV ( 1990). Odontoblasts: developmental aspects. In: Dynamic aspects of the dental pulp. Iuoki R, Kudo T, Olgart L, editors. London: Chapman and Hall, pp. 29-50.
  • Rutherford RB, Wahle J., Tucker M., Rueger D., Charette M. (1993). Induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-I. Arch Oral Biol 38:571-576.[CrossRef][Medline] [Order article via Infotrieve]
  • Schroder U., Granath LE (1971). Early reaction of intact human teeth to calcium hydroxide following experimental pulpotomy and its significance to the develoment of hard tissue barrier. Odontol Revy 22:379-395.[Medline] [Order article via Infotrieve]
  • Schroder U., Sundstrom B. (1974). Transmission electron microscopy of tissue changes following experimental pulpotomy of intact human teeth and capping with calcium hydroxide. Odontol Revy 25:57-67.[Medline] [Order article via Infotrieve]
  • Sluka H., Lehmann R., Flores de Jacoby L. (1979). Verwendung von organischer Knochenmatrix als Material fur die direkte Uberkappung der Pulpa. Dtsch Zahnädrztl Z 34:467-469.
  • Somerman MJ, Nathanson MA, Sauk JJ, Manson B. (1987). Human dentin matrix induces cartilage formation in vitro by mesenchymal cells derived from embryonic muscle. J Dent Res 66:1551-1558.
  • Tziafas D., Kolokuris I. (1990). Inductive influences of demineralized dentin and bone matrix on pulp cells: an approach of secondary dentinogenesis. J Dent Res 69:75-81.
  • Tziafas D., Kolokuris I., Alvanou A., Kaidoglou K. (1992). Short-term dentinogenic response of dog dental pulp tissue after its induction by demineralized or native dentine, or predentine. Arch Oral Biol 37:119-128.[Medline] [Order article via Infotrieve]
  • Vainio S., Karavanova I., Jowett A., Thesleff I. (1993). Identification of BMP-4 as a signal mediating secondary induction between epithelial and mesenchymal tissues during early tooth development. Cell 75:45-58.[CrossRef][Medline] [Order article via Infotrieve]
  • Veis A. ( 1985). The role of dental pulp-thoughts on the session on pulp repair processes. J Dent Res 64:552-554.
  • Wang EA, Rosen V., D'Alessandro JS, Bauduy M., Cordes P., Harada T., et al. (1990). Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci USA 87:2220-2224.[Abstract/Free Full Text]
  • Wozney JM, Rosen V., Celeste AJ, Mitsock LM, Whitters MJ, Kriz RW, et al. (1988). Novel regulators of bone formation: molecular clones and activities. Science 242:1528-1534.[Abstract/Free Full Text]
  • Yamamura T. (1985). Differentiation of pulp cell and inductive influences of various matrices with reference to pulp wound healing. J Dent Res 64:530-540.[Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 73, No. 9, 1515-1522 (1994)
DOI: 10.1177/00220345940730090601
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 CROBMHome page
M. Goldberg and A. J. Smith
CELLS AND EXTRACELLULAR MATRICES OF DENTIN AND PULP: A BIOLOGICAL BASIS FOR REPAIR AND TISSUE ENGINEERING
Critical Reviews in Oral Biology & Medicine, January 1, 2004; 15(1): 13 - 27.
[Abstract] [Full Text] [PDF]

Home page
 ADRHome page
A.J. Smith, P.E. Murray, A.J. Sloan, J.B. Matthews, and S. Zhao
Trans-dentinal Stimulation of Tertiary Dentinogenesis
Advances in Dental Research, August 1, 2001; 15(1): 51 - 54.
[Abstract] [PDF]

Home page
 ADRHome page
I. About and T. A. Mitsiadis
Molecular Aspects of Tooth Pathogenesis and Repair: in vivo and in vitro Models
Advances in Dental Research, August 1, 2001; 15(1): 59 - 62.
[Abstract] [PDF]

Home page
 ADRHome page
Y. Nakamura, L. Hammarstrom, E. Lundberg, H. Ekdahl, K. Matsumoto, S. Gestrelius, and S.P. Lyngstadaas
Enamel Matrix Derivative Promotes Reparative Processes in the Dental Pulp
Advances in Dental Research, August 1, 2001; 15(1): 105 - 107.
[Abstract] [PDF]

Home page
 ADRHome page
H. Lovschall, O. Fejerskov, and A. Flyvbjerg
Pulp-capping with Recombinant Human Insulin- like Growth Factor I (rhIGF-I) in Rat Molars
Advances in Dental Research, August 1, 2001; 15(1): 108 - 112.
[Abstract] [PDF]

Home page
 CROBMHome page
A.J. Smith and H. Lesot
Induction and Regulation of Crown Dentinogenesis: Embryonic Events as a Template for Dental Tissue Repair?
Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(5): 425 - 437.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Li, P. M. Bartold, C. Z. Zhang, R. W. Clarkson, W. G. Young, and M. J. Waters
Growth Hormone and Insulin-Like Growth Factor I Induce Bone Morphogenetic Proteins 2 and 4: A Mediator Role in Bone and Tooth Formation?
Endocrinology, September 1, 1998; 139(9): 3855 - 3862.
[Abstract] [Full Text] [PDF]

Home page
 JDRHome page
M.N. Helder, H. Karg, T.J.M. Bervoets, S. Vukicevic, E.H. Burger, R.N. D'Souza, J.H.M. Woltgens, G. Karsenty, and A.L.J.J. Bronckers
Bone Morphogenetic Protein-7 (Osteogenic Protein-1, OP-1) and Tooth Development
Journal of Dental Research, April 1, 1998; 77(4): 545 - 554.
[Abstract] [PDF]

Home page
 JDRHome page
K. Yoshiba, N. Yoshiba, H. Nakamura, M. Iwaku, and H. Ozawa
Immunolocalization of Fibronectin during Reparative Dentinogenesis in Human Teeth after Pulp Capping with Calcium Hydroxide
Journal of Dental Research, August 1, 1996; 75(8): 1590 - 1597.
[Abstract] [PDF]

Home page
 JDRHome page
T. Zelles, K.R. Purushotham, S.P. Macauley, G.E. Oxford, and M.G. Humphreys-Beher
Concise Review: Saliva and Growth Factors: The Fountain of Youth Resides in Us All
Journal of Dental Research, December 1, 1995; 74(12): 1826 - 1832.
[Abstract] [PDF]

Home page
 CROBMHome page
B. Rutherford and M. Fitzgerald
A New Biological Approach To Vital Pulp Therapy
Critical Reviews in Oral Biology & Medicine, January 1, 1995; 6(3): 218 - 229.
[Abstract] [Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Nakashima, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nakashima, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?