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Transforming Growth Factor-beta-1 Reduces Alkaline Phosphatase mRNA and Activity and Stimulates Cell Proliferation in Cultures of Human Pulp Cells

Departments of Endodontology and Periodontology, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

H. Shiba

Departments of Endodontology and Periodontology, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

K. Nakanishi

Departments of Endodontology and Periodontology, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

T. Ogawa

Departments of Endodontology and Periodontology, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

H. Okamoto

Departments of Endodontology and Periodontology, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

K. Nakashima

Department of Biochemistry, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

M. Noshiro

Department of Biochemistry, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

Y. Kato

Department of Biochemistry, Hiroshima University School of Dentistry, 2-3, Kasumi 1-Chome, Minamiku, Hiroshima 734, Japan

Transforming growth factor-beta-1 (TGF-beta-1) is a potent modulator of proliferation and differentiation in various tissues, and may be involved in the control of dental development and repair. This study was carried out to investigate the effects of TGF-beta-1 on alkaline phosphatase (ALPase) activity and mRNA level, and on DNA content in cultures of human pulp cells. Four lines of pulp cells (P1-P4), isolated from the upper wisdom teeth of four patients, were maintained separately in monolayer cultures in the presence of 10% fetal bovine serum. TGF-beta-1, at 0.1 ng/mL, increased ALPase activity and DNA content in P1 cultures, but not in P2-P4 cultures. In all cultures, TGF-beta-1, at 5 ng/mL, decreased ALPase activity to a very low level, and increased DNA content. Northern analysis showed that human pulp cells synthesized a single species of 2.6-kb liver/bone/kidney-type ALPase, and that TGF-beta-1, at 5 ng/mL, decreased the level of the ALPase mRNA. These results suggest that TGF-beta-1 is a mitogen for human pulp cells, and that it regulates the activity of the universal-type ALPase at the pre-translational level.

Key Words: TGF-beta • Alkaline Phosphatase • Pulp Cells • mRNA.

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Journal of Dental Research, Vol. 73, No. 9, 1509-1514 (1994)
DOI: 10.1177/00220345940730090501


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