This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ni Eidhin, D. Articles by Mouton, C. Search for Related Content PubMed PubMed Citation Articles by Ni Eidhin, D. Articles by Mouton, C. Social Bookmarking                         What's this?

The Lipopolysaccharide of Porphyromonas gingivalis is not Antigenically Cross-reactive with that of Other Species

Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada G1K 7P4

C. Mouton

Groupe de Recherche en Ecologie Buccale, Faculte de Medecine Dentaire, Universite Laval, Quebec, Canada G1K 7P4

Large numbers of Porphyromonas, Prevotella, and Bacteroides strains were screened by 3 monoclonal antibodies (MAbs) and 8 rabbit antisera raised against Porphyromonas gingivalis, in order to detect any possible recognition of non-P. gi ngivalis surf ace antigens by these immunoreagents. All three MAbs, which were LPS-specific, extensively recognized LPS from 10 P. gingivalis strains in immunoblotting, whereas they recognized none of the 34 non-P. gingivalis strains. Rabbit antisera were similarly specific for P. gingivalis cells in immunofluorescence and with LPS in grid-blotting, but several of them recognized LPS from one Prevotella melaninogenica and 5 Prevotella intermedia strains in Western blotting. Since several pre-immune sera and an irrelevant serum raised to a Streptococcus species recognized up to 5 of these preparations, we exclude that the reactions were due to antigens shared by P. gingivalis and Prevotella. Rather, we consider that they were false-positive reactions due to natural antibodies, stimulated in a non-specific manner upon immunization with P. gingivalis, in animals whose immune systems were sensitized to Prevotella species before immunization.

Key Words: Antibodies • Bacterial • Monoclonal • LPS • Porphyromonas gingivalis

REFERENCES

• Ansorge W. (1985). Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate. J Biochem Biophys Meth 11:13-20.[CrossRef][Medline] [Order article via Infotrieve]
• Bogard WCJ, Dunn DL, Abernethy K., Kilgarif C., Kung PC ( 1987). Isolation and characterization of murine monoclonal antibodies specific for Gram-negative bacterial lipopolysaccharide: association of cross-genus reactivity with lipid A specificity. Infect Immun 55:899-908.[Abstract/Free Full Text]
• Bowden GHW, Nolette N. (1990). Study of the antigenic relationships between strains of Bacteroides intermedius, B. melaninogenicus, B. corporis, and B.denticola revealed by immunoblotting with rabbit antisera. Can J Microbiol 36:637-648.[Medline] [Order article via Infotrieve]
• Chandad F., Mouton C. (1990). Molecular size variation of the hemagglutinating adhesin HA-Ag2, a common antigen of Bacteroides gingivalis. Can J Microbiol 36:690-696.[Medline] [Order article via Infotrieve]
• Chen PB, Bochacki V., Reynolds HS, Beanan J., Tatakis DN, Zambon JJ, et al. (1986). The use of monoclonal antibodies to detect Bacteroides gingivalis in biological samples. Infect Immun 54:798-803.[Abstract/Free Full Text]
• Dahlen G., Mattsby-Baltzer I. (1983). Lipid A in anaerobic bacteria. Infect Immun 39:466-468.[Abstract/Free Full Text]
• Dale PA, McQuillen DP, Gulati S., Rice PA ( 1992). Human vaccination with Escherichia coli J5 mutant induces cross-reactive bactericidal antibody against Neisseriagonorrhoeae lipooligosaccharide. J Infect Dis 166:316-325.[Medline] [Order article via Infotrieve]
• Deslauriers M., Mouton C. (1990). Immunoreactivity in humans of Bacteroides gingivalis hemagglutinating adhesin HA-Ag2. Oral Microbiol Immunol 5:302-304.[Medline] [Order article via Infotrieve]
• Deslauriers M., Mouton C. (1991). Targetting the production of monoclonal antibodies to the hemagglutinating adhesin of Bacteroides gingivalis by injection of an immunoprecipitate from crossed immunoelectrophoresis. J Biol Buccale 19:155-160.[Medline] [Order article via Infotrieve]
• Deslauriers M., Ni Eidhin D., Lamonde L., Mouton C. (1990). SDS-PAGE analysis of protein and lipopolysaccharide of extracellular vesicules and Sarkosyl-insoluble membranes from Bacteroides gingivalis. Oral Microbiol Immunol 5:1-7.[Medline] [Order article via Infotrieve]
• Ebersole JL, Taubman MA, Smith DJ, Frey DE ( 1988). Serological classification of Bacteroides from the human oral cavity. J Periodont Res 23:22-27.[Medline] [Order article via Infotrieve]
• Gmur R., Guggenheim B. (1983). Antigenic heterogeneity of Bacteroides intermedius as recognized by monoclonal antibodies. Infect Immun 42:459-470.[Abstract/Free Full Text]
• Hamada S., Takada H., Ogawa T., Fujiwara T., Mihara J. (1990). Lipopolysaccharides of oral anaerobes associated with chronic inflammation: chemical and immunomodulating properties. Int Rev Immunol 6:247-261.[Medline] [Order article via Infotrieve]
• Hanazawa S., Saitoh K., Ohmori Y., Nishihara H., Fujiwara S., Kitano S. (1984). Production of monoclonal antibodies that recognize specific and cross-reactive antigens of Bacteroides gingivalis. Infect Immun 46:285-287.[Abstract/Free Full Text]
• Hitchcock PJ, Brown TM (1983). Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154:269-277.[Abstract/Free Full Text]
• Laemmli UK (1970). Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature ( London) 227:680-686.[CrossRef][Medline] [Order article via Infotrieve]
• Millar SJ, Chen PB, Hausmann E. (1987). Monoclonal antibody for identification of Bacteroides gingivalis lipopolysacchahde. J Clin Microbiol 25:2437-2439.[Abstract/Free Full Text]
• Mouton C., Hammond PG, Slots J., Reed MJ, Genco RJ (1981). Identification of Bacteroides gingivalis by fluorescent antibody staining. Ann Microbiol 132(B):69-83.
• Naito Y., Okuda K., Kato T., Takazoe I. (1985). Monoclonal antibodies against surface antigens of Bacteroides gingivalis. Infect Immun 50:231-235.[Abstract/Free Full Text]
• Nakazawa F., Zambon JJ, Reynolds HS, Genco RJ (1988). Serological studiesof oral Bacteroides intermedius. Infect Immun 56:1647-1651.[Abstract/Free Full Text]
• Ni Eidhin D., Mouton C. (1993). A rapid method for preparation of rough and smooth lipopolysaccharide from Bacteroides, Porphyromonas and Prevotella. FEMS Microbiol Lett 110:133-138.[CrossRef][Medline] [Order article via Infotrieve]
• Okuda K., Ohta K., Kato T., Takazoe I., SlotsJ. ( 1986). Antigenic characteristics and serological identification of 10 black-pigmented Bacteroides species. J Clin Microbiol 24:89-95.[Abstract/Free Full Text]
• Parent R., Mouton C., Lamonde L., Bouchard D. (1986). Human and animal serotypes of Bacteroides gingivalis defined by crossed immunoelectrophoresis. Infect Immun 51:909-918.[Abstract/Free Full Text]
• Reed MJ, Slots J., Mouton C., Genco RJ (1980). Antigenic studies of oral and non-oral black-pigmented Bacteroides strains. Infect Immun 29:564-574.[Abstract/Free Full Text]
• Seow WK, Bird PS, Seymour GJ, Thong YH (1989). Modulation of human neutrophil adherence by periodontopathic bacteria: Reversal by specific monoclonal antibodies. Int Arch Allergy Appl Immunol 90:24-30.[Medline] [Order article via Infotrieve]
• Shah HN, Collins MD (1990). Prevotella, a new genus to include Bacteroides melaninogenicus and related species formerly classified in the genus Bacteroides. Int J System Bacteriol 40:205-208.[Abstract/Free Full Text]
• Shah HN, Collins MD (1988). Proposal forreclassification of Bacteroides asaccharolyticus, Bacteroides gingivalis and Bacteroides endodontalis in a new genus, Porphyromonas. Int J System Bacteriol 38:128-131.[Abstract/Free Full Text]
• Shah HN, Collins MD (1989). Proposal to restrict the genus Bacteroides (Castellani and Chalmers) to Bacteroides fragilis and closely related species. Int J System Bacteriol 39:85-87.[Abstract/Free Full Text]
• Shepertycky MR, Bowden GHW (1991). Antigenic relationships between outer membranes of serogroups of Prevotella intermedius and their cross-reaction with other Prevotella and Porphyromonas (abstract). J Dent Res 70:501.
• Shepertycky MR, Bowden GHW (1990). Mouse antibody to Bacteroides intermedius and cross-reactivity to different Bacteroides (abstract). J Dent Res 69:139.
• Simonson LG, Merrell BR, Rouse RF, Shklair IL (1986). Production and characterization of monoclonal antibodies to Bacteroides gingivalis. J Dent Res 65:95-97.
• Sixou J-L. (1992). Bacteroides (Porphyromonas) gingivalis et autres pathogenes parodontaux dans la cavite buccale de l'enfant (PhD thesis). Universite de Rennes, France.
• Suzuki Y., Yoshimura F., Takahashi K., Tani H., Suzuki T. (1988). Detection of fimbriae and fimbrial antigens in the oral anaerobe Bacteroides gingivalis by negative staining and serological methods. J Gen Microbiol 134:2713-2720.[Abstract/Free Full Text]
• Wolff LF, Anderson L., Sandberg GP, Aeppli DM, Shelburne CE (1991). Fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque. J Clin Microbiol 29:1645-1651.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 73, No. 3, 661-670 (1994)
DOI: 10.1177/00220345940730031201


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K.-A. Nguyen, A. A. DeCarlo, M. Paramaesvaran, C. A. Collyer, D. B. Langley, and N. Hunter Humoral Responses to Porphyromonas gingivalis Gingipain Adhesin Domains in Subjects with Chronic Periodontitis Infect. Immun., March 1, 2004; 72(3): 1374 - 1382. [Abstract] [Full Text] [PDF]

				M. Bhatti, A. MacRobert, B. Henderson, P. Shepherd, J. Cridland, and M. Wilson Antibody-Targeted Lethal Photosensitization of Porphyromonas gingivalis Antimicrob. Agents Chemother., October 1, 2000; 44(10): 2615 - 2618. [Abstract] [Full Text]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Ni Eidhin, D. Articles by Mouton, C. Search for Related Content PubMed PubMed Citation Articles by Ni Eidhin, D. Articles by Mouton, C. Social Bookmarking                         What's this?