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Immunocytochemical Localization of Blood Group ABH Antigens in the Human Parotid Gland

Dipartimento di Citomorfologia, Universita di Cagliari, via Porcell 2, 09124 Cagliari, Italy

M. Cossu

Dipartimento di Citomorfologia, Universita di Cagliari, via Porcell 2, 09124 Cagliari, Italy

The aim of this study was to determine whether the parotid gland, like other salivary glands, produces blood group antigens. A post-embedding immunogold staining (IGS) method for ABH antigens was applied to osmicated and non-osmicated, routinely prepared specimens of normal human parotid glands. A and B antigens were not detected, whereas H antigen was found in acini and ducts of glands from A, B, and O subjects. In osmicated samples, the only sites of H labeling were secretory granules of a few acinar cells and those of most intercalated duct cells. In both cell types, only the pale matrix of the granules was stained. In non-osmicated samples, secretory granules showed the same distribution pattern of H, although gold particles were more abundant, and basolateral membranes of acinar and striated duct cells and the apical vesicles of striated duct cells were also labeled. Our results suggest that small amounts of H antigen are secreted by the parotid gland.

Key Words: Electron Microscopy • Immunohistochemistry • Parotid Gland

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Journal of Dental Research, Vol. 73, No. 2, 556-560 (1994)
DOI: 10.1177/00220345940730021101


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Lantini, M.S. Articles by Cossu, M. Search for Related Content PubMed PubMed Citation Articles by Lantini, M.S. Articles by Cossu, M. Social Bookmarking                         What's this?