Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Dental Research
This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Nagai, M.
Right arrow Articles by Ota, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagai, M.
Right arrow Articles by Ota, M.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Electromagnetic Fields
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Pulsating Electromagnetic Field Stimulates mRNA Expression of Bone Morphogenetic Protein-2 and -4

M. Nagai

Department of Biochemistry, Iwate Medical University School of Dentistry, Morioka, Iwate 020, Japan

M. Ota

Department of Biochemistry, Iwate Medical University School of Dentistry, Morioka, Iwate 020, Japan

The effects of a pulsating electromagnetic field on mRNA expression of bone morphogenetic protein-2 and -4 in chick embryonic calvaria were examined. From the onset of embryogenesis (Day 0), chick embryos were incubated in a continuously generated pulsating electromagnetic field with a peak of 3.5 milli-Tesla (mean: 2 milli-Tesla) and vibration at 15 Hz. Control chicks were incubated in a normal magnetic field. Northern-blot analysis showed that the mRNAs of bone morphogenetic protein-2 and -4 were expressed in the calvaria. Quantitative analysis of the mRNA expressions was done by means of slot-blot hybridization. The magnetic field enhanced the expressions of both mRNAs. The enhancements were more pronounced in younger chick embryos (Day 15 > Day 17), and no significant change was observed in the 19-day-old embryos. These results indicate that osteo-inductive effects of the magnetic field were mediated at least in part by bone morphogenetic protein-2 and -4.

Key Words: Bone and Bones • Extracellular Matrix Proteins • Bone Morphogenetic Protein • Electromagnetic Field.

REFERENCES

  • Alonso S., Minty A., Boulet Y., Buckingham M. (1989). Comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates. J Mol Evol 23:11-22.
  • Ashihara T., Kagawa K., Kamachi M., Inoue S., Ohashi T., Takeoka 0 (1979). 3H-thymidine autoradiographic studies of the cell proliferation and differentiation in the electrically stimulated osteogenesis. In: Electrical properties of bone and cartilage. Brighton CT, Black J, Pollack SR, editors. New York: Grune and Stratton, pp. 401-426.
  • Bassett Cal, Pawluk RJ, Pilla AA (1974a). Augmentation of bone repair by inductively-coupled electromagnetic fields. Science 184:575-577.[Abstract/Free Full Text]
  • Bassett Cal, Pawluk RJ, Pilla AA (1974b). Acceleration of bone repair by electromagnetic fields (a surgically non-invasive method). Ann NY Acad Sci 238:242-262.[Medline] [Order article via Infotrieve]
  • Bassett Cal, Pilla AA, Pawluk RJ (1977). A non-operative salvage of surgically-resistant pseudarthroses and non-unions by pulsing electromagnetic fields. A preliminary report. Clin Ort hop 124:128-143.
  • Bassett Cal, Chokshi HR, Hernandez E., Pawluk RJ, Strop M. (1979). The effect of pulsing electromagnetic fields on cellular calcium and calcification of nonunions. In: Electrical properties of bone and cartilage. Brighton CT, Black J, Pollack SR, editors. New York: Grune and Stratton, pp. 427-441.
  • Bassett Cal, Mitchell SN, Gaston SR (1981). Treatment of ununited tibial diaphyseal fractures with pulsing electromagnetic fields. J Bone Joint SurgAm 63:511-523.[Abstract/Free Full Text]
  • Brighton CT, Friedenberg ZB, Black J., Esterhai JL, Mitchell JE, Montique F. (1981). Electrically induced osteogenesis: relationship between charge, current density, and the amount of bone formed: introduction of a new cathode concept. Clin Orthop 161:122-132.
  • Brighton CT, Unger AS, Stambough JL (1984). In vitro growth of bovine articular cartilage chondrocytes in various capacitively coupled electrical fields. J Orthop Res 2:15-22.[CrossRef][Medline] [Order article via Infotrieve]
  • Chen TL, Bates RL, Dudley A., Hammonds RG, Amento EP (1991). Bone morphogenetic protein-2B stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: comparison with TGF-1. J Bone Mineral Res 6:1387-1393.[Medline] [Order article via Infotrieve]
  • Chomczynski P., Sacchi N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal Biochem 162:156-159.[Medline] [Order article via Infotrieve]
  • Colacicco G., Pilla AA (1984). Electromagnetic modulation of biological processes: influence of culture media and significance of methodology in the Ca-uptake by embryonal chick tibia in vitro. Calcif Tissue Int 36:167-174.[Medline] [Order article via Infotrieve]
  • Connolly JF, Hahn H., Jardon OM (1977). The electrical enhancement of periosteal proliferation in normal and delayed fracture healing. Clin Orthop 124:97-105.
  • Cossarizza A., Monti D., Bersani F., Paganelli R., Montagnani G., Cadossi R., et al. (1989). Extremely low frequency pulsed electromagnetic fields increase interleukin-2(IL-2) utilization and IL-2 receptor expression in mitogen-stimulated human lymphocytes from old subjects. FEBSLett 248:141-144.[Medline] [Order article via Infotrieve]
  • Cossarizza A., Angioni S., Petraglia F., Genazzani AR, Monti D., Capri M., et al. (1993). Exposure to low frequency pulsed electromagnetic fields increases interleukin-1 and interleukin-6 production by human peripheral blood mononuclear cells. Exp Cell Res 204:385-387.[CrossRef][Medline] [Order article via Infotrieve]
  • Edwall D., Prisell PT, Levinovitz A., Jennische E., Norstedt G. (1992). Expression of insulin-like growth factor 1 messenger ribonucleic acid in regenerating bone after fracture: influence of indomethacin. J Bone Miner Res 7:207-213.[Medline] [Order article via Infotrieve]
  • Fitzsimmons RJ, Farley J., Adey WR, Baylink DJ (1986). Embryonic bone matrix formation is increased after exposure to a low-amplitude capacitively coupled electric field, in vitro. Biochem Biophys Acta 882:51-56.[Medline] [Order article via Infotrieve]
  • Goodman R., Henderson AS (1988). Exposure of salivary gland cells to low-frequency electromagnetic fields alters polypeptide synthesis. Proc Natl Acad Sci USA 85:3928-3932.[Abstract/Free Full Text]
  • Hiraki Y., Inoue H., Shigeno C., Sanma Y., Bentz H., Rosen DM, et al. (1991). Bone morphogenetic proteins (BMP-2 and BMP-3) promote growth and expression of the differentiated phenotype of rabbit chondrocytes and osteoblastic MC3T3-El cells in vitro. J Bone Miner Res 6:1373-1385.[Medline] [Order article via Infotrieve]
  • Hulth A. (1989). Basic science and pathology: current concepts of fracture healing. Clin Orthop 249(Sect III):265-284.[Medline] [Order article via Infotrieve]
  • Hulth A., Johnell 0, Henricson A. (1988). The implantation of demineralized fracture matrix yields more new bone formation than does intact matrix. Clin Orthop 234:235-239.[Medline] [Order article via Infotrieve]
  • Jingushi S., Heydemann A., Kana SK, Macey LR, Bolander ME (1990). Acidic fibroblast growth factor injection stimulates cartilage enlargement and inhibits cartilage gene expression in rat fracture healing. Orthop Res 8:364-371.
  • Joyce ME, Jingushi S., Bolander ME (1990). Transforming growth factor β in the regulation of bone repair. Orthop Clin North Am 21:199-209.[Medline] [Order article via Infotrieve]
  • Katagiri T., Yamaguchi A., Ikeda T., Yoshiki S., Wozney JM, Rosen V., et al. (1990). The non-osteogenic mouse pluripotent cell line, C3H10T1/2, is induced to differentiate into osteoblastic cells by recombinant bone morphogenetic protein-2. Biochem Biophys Res Commun 172:295-299.[CrossRef][Medline] [Order article via Infotrieve]
  • Luyten FP, Cunningham NS, Vukicevic S., Paralkar V., Ripamonti U., Reddi AH (1992). Advances in osteogenin and related bone morphogenetic proteins in bone induction and repair. Acta Orthop Belg 58(Suppl 1):263-267.
  • Lyons KM, Pelton RW, Hogan Blm (1990). Organogenesis and pattern formation in the mouse: RNA distribution patterns suggest a role for bone morphogenetic protein-2A (BMP-2A). Development 109:833-844.[Abstract/Free Full Text]
  • Nishimatsu S., Suzuki A., Shoda A., Murakami K., Ueno N. ( 1992). Genes for bone morphogenetic proteins are differentially transcribed in early amphibian embryos. Biochem Biophys Res Commun 186:1487-1495.[CrossRef][Medline] [Order article via Infotrieve]
  • Norton LA, Rovetti LA (1988). Calcium incorporation in cultured chondroblasts perturbed by an electromagnetic field. J Orthop Res 6:559-566.[Medline] [Order article via Infotrieve]
  • Norton LA, Bourret LA, Majeska RJ, Rodan GA (1979). Adherence and DNA synthesis changes in hard tissue cell culture produced by electric perturbation. In: Electrical properties of bone and cartilage. Brighton CT, Black J, Pollack SR, editors. New York: Grune and Stratton, pp. 443-454.
  • Norton LA, Shteyer A., Rodan GA (1980). Electromagnetic field effects on DNA synthesis in bone cells. J Electrochem Soc 127:129C.[CrossRef]
  • Ohta S., Yamamuro T., Lee K., Okuma H., Kasai R., Hiraki Y., et al. (1991). Fracture healing induces expression of the proto-oncogene c-fos in vivo: possible involvement of the Fos protein in osteoblastic differentiation. FEBSLett 284:42-45.[CrossRef][Medline] [Order article via Infotrieve]
  • Ozkaynk E., Rueger DC, Drier EA, Corbett C., Ridge RJ, Sampath TK, et al. (1990). OP-1 cDNA encodes an osteogenic protein in the TGF-p family. ENBO J 9:2085-2093.[Medline] [Order article via Infotrieve]
  • Rodan GA, Bourret LA, Norton LA (1978). DNA synthesis in cartilage cells is stimulated by oscillating electric fields. Science 199:690-692.[Abstract/Free Full Text]
  • Rosen V., Thies RS (1992). The BMP proteins in bone formation and repair. Trends Genet 8(3):97-102.[Medline] [Order article via Infotrieve]
  • Sakano S., Murata Y., Miura T., Iwata H., Sato K., Matsui N., et al. (1993). Collagen and alkaline phosphatase gene expression during bone morphogenetic protein (BMP)-induced cartilage and bone differentiation. Clin Orthop 292:337-344.
  • Takano-Yamamoto T., Kawakami M., Sakuda M. (1992). Effect of a pulsing electromagnetic field on demineralized bone-matrix-induced bone formation in a bony defect in the premaxilla of rats. J Dent Res 71:1920-1925.
  • Takuwa Y., Ohse C., Wang EA, Wozney JM, Yamashita K. (1991). Bone morphogenetic protein-2 stimulates alkaline phosphatase activity and collagen synthesis in cultured osteoblastic cells, MC3T3-El. Biochem Biophys Res Commun 174:96-101.[CrossRef][Medline] [Order article via Infotrieve]
  • Thies RS, Bauduy M., Ashton BA, Kurtzberg L., Wozney JM, Rosen V. (1992). Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells. Endocrinol 130:1318-1324.[Abstract/Free Full Text]
  • Vukicevic S., Luyten FP, Reddi AH (1989). Stimulation of the expression of osteogenic and chondrogenic phenotypes in vitro by osteogenin. Proc Natl Acad Sci USA 86:8793-8797.[Abstract/Free Full Text]
  • Vukicevic S., Luyten FP, Reddi AH (1990). Osteogenin inhibits proliferation and stimulates differentiation in mouse osteoblast-like cells (MC3T3-El). Biochem Biophys Res Commun 166:750-756.[CrossRef][Medline] [Order article via Infotrieve]
  • Wang EA, Rosen V., D'Alessandro JS, Bauduy M., Cordes P., Harada T., et al. (1990). Recombinant human bone morphogenetic protein induces bone formation. Proc Natl Acad Sci USA 87:2220-2224.[Abstract/Free Full Text]
  • Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C., Raducha M., Harris H. (1986). Isolation and characterization of a cDNA encoding a human liver/bone/kidney-type alkaline phosphatase. Proc Natl Acad Sci USA 83:7182-7186.[Abstract/Free Full Text]
  • Wozney JM (1992). The bone morphogenetic protein family and osteogenesis. Molec Reprod Dev 32:160-167.
  • Wozney JM, Rosen V., Celeste AJ, Mitsock Clm, Whitters MJ, Kriz RW, et al. (1988). Novel regulators of bone formation: molecular clones and activities. Science 242:1528-1534.[Abstract/Free Full Text]
  • Yamaguchi A., Katagiri T., Ikeda T., Wozney JM, Rosen V., Wang EA, et al. (1991). Recombinant human bone morphogenetic protein-2 stimulates osteoblastic maturation and inhibits myogenic differentiation in vitro. J Cell Biol 113:681-687.[Abstract/Free Full Text]
  • Zusman I., Yaffe P., Pinus H., Ornoy A. (1990). Effects of pulsing electromagnetic fields on the prenatal and postnatal development in mice and rats: in vivo and in vitro studies. Teratol 42:157-170.

Journal of Dental Research, Vol. 73, No. 10, 1601-1605 (1994)
DOI: 10.1177/00220345940730100401
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 JBJSHome page
Z. Wang, C. C. Clark, and C. T. Brighton
Up-Regulation of Bone Morphogenetic Proteins in Cultured Murine Bone Cells with Use of Specific Electric Fields
J. Bone Joint Surg. Am., May 1, 2006; 88(5): 1053 - 1065.
[Abstract] [Full Text] [PDF]

Home page
 J Am Acad Orthop SurgHome page
F. R. T. Nelson, C. T. Brighton, J. Ryaby, B. J. Simon, J. H. Nielson, D. G. Lorich, M. Bolander, and J. Seelig
Use of Physical Forces in Bone Healing
J. Am. Acad. Ortho. Surg., September 1, 2003; 11(5): 344 - 354.
[Abstract] [Full Text] [PDF]

Home page
 INT J LOW EXTREM WOUNDSHome page
S. A.W. Pickering and B. E. Scammell
Electromagnetic Fields for Bone Healing
International Journal of Lower Extremity Wounds, September 1, 2002; 1(3): 152 - 160.
[Abstract] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Nagai, M.
Right arrow Articles by Ota, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagai, M.
Right arrow Articles by Ota, M.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Medline Plus Health Information
*Electromagnetic Fields
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?