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Effect of Centrifugal Force on Growth of Mouse Osteoblastic MC3T3-E1 Cells in vitro

Department of Orthodontics

O. Nakamura

Department of Orthodontics

Y. Duan

Department of Orthodontics, Qindu Stomatological College, Xian, Shaanxi, China

Y. Hiraki

Biochemistry, Osaka University Faculty of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565, Japan

M. Sakuda

Department of Orthodontics

The effect of biomechanical force on growth of skeletal tissue was studied in monolayer cultures of mouse osteoblastic MC3T3-E1 cells which were centrifuged at 320 g for 15 min to 72 h in a CO₂ incubator. Centrifugation of the cells for 30 min in low concentrations (0.3 or 1%) of fetal bovine serum (FBS) caused a two-fold increase of [³H]thymidine incorporation at 20 h from the start of centrifugation. However, centrifugation under 10% FBS caused no increase in [3H]thymidine incorporation into DNA. Under 0.3% FBS, [3H]thymidine incorporation increased in a manner dependent on the period of centrifugation and reached a maximum when the cells were centrifuged for 3 h. Stimulation of DNA synthesis by centrifugation was abolished in the presence of H-7, an inhibitor of protein kinase C. Moreover, conditioned medium collected from the centrifuged cultures increased [³H]thymidine incorporation by two-fold over the basal when added to a quiescent culture of MC3T3-E1 cells. These results suggest that centrifugal force stimulates growth of osteoblastic cells through autocrine secretion of some diffusible growth-promoting activity. On the other hand, centrifugation of the cells inhibited induction by FBS of alkaline phosphatase activity and calcium-uptake, two indices of the differentiated phenotype of osteoblasts.

REFERENCES

• Bessay OA, Lowry OH, Brock MJ (1946). A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J Biol Chem 164:321-329.[Free Full Text]
• Binderman I., Shimshoni Z., Somjen D. (1984). Biochemical pathways involved in the translation of physical stimulus into biological message. Calcif Tissue Int 36(Suppl):82S-85S.[CrossRef][Medline] [Order article via Infotrieve]
• Burger EH, Veldhuijzen JP, Klein Nulend J., van Loon Jjwa (1989). Osteoclastic invasion and mineral resorption of fetal mouse long bone rudiments are inhibited by culture under intermittent compressive force. Connect Tissue Res 20:131-141.[CrossRef][Medline] [Order article via Infotrieve]
• Duke JC ( 1983). Suppression of morphogenesis in embryonic mouse limbs exposed in vitro to excess gravity. Teratology 27:427-436.[CrossRef][Medline] [Order article via Infotrieve]
• Globus RK, Bikle DD, Morey-Holton E. (1986). The temporal response of bone to unloading. Endocrinol 118:733-742.[Abstract/Free Full Text]
• Hakeda Y., Nakatani Y., Kurihara N., Ikeda E., Maeda N., Kumegawa M. (1985). Prostaglandin E2 stimulates collagen and non-collagen protein synthesis and prolyl hydroxylase activity in osteoblastic clone MC3T3-E1 cells. Biochem Biophys Res Commun 126:340-345.[CrossRef][Medline] [Order article via Infotrieve]
• Hasegawa S., Sato S., Saito S., Suzuki Y., Brunette DM (1985). Mechanical stretching increases the number of cultured bone cells synthesizing DNA and alters their pattern of protein synthesis. Calcif Tissue Int 37:431-436.[Medline] [Order article via Infotrieve]
• Inoue H., Hiasa K., Samma Y., Nakamura 0, Sakuda M., Iwamoto M., et al. (1990). Stimulation of proteoglycan and DNA syntheses in chondrocytes by centrifugation. J Dent Res 69:1560-1563.[Abstract/Free Full Text]
• Klein-Nulend J., Veldhuijzen JP, de Jong M., Burger EH (1987). Increased bone formation and decreased bone resorption in fetal mouse calvaria as a result of intermittent compressive force in vitro. Bone Mineral 2:441-448.[Medline] [Order article via Infotrieve]
• Klein-Nulend J., Veldhuijzen JP, van Strien ME, de Jong M., Burger EH (1990). Inhibition of osteoclastic bone resorption by mechanical stimulation in uitro. Arthritis Rheum 33:66-72.[CrossRef][Medline] [Order article via Infotrieve]
• Kodama H., Amagai Y., Sudo H., Kasai S., Yamamoto S. (1981). Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jpn J Oral Biol 23:899-901.
• Komori A., Suzuki H. (1991). Effect of centrifugal force on human osteoblast-like cells. J Jpn Orthod Soc 50:448-457.
• Morey EM, Baylink DJ (1979). Inhibition of bone formation during space flight. Science 201:1138-1141.[CrossRef]
• Murray DW, Ruston N. (1990). The effect of strain on bone cell prostaglandin E, release: a new experimental method. Calcif Tissue Int 47:35-39.[Medline] [Order article via Infotrieve]
• Palmoski M., Perricone E., Brandt KD (1979). Development and reversal of a proteoglycan aggregation defect in normal canine knee cartilage after immobilization. Arthritis Rheum 22:509-517.
• Palmoski MJ, Colyer RA, Brandt KD (1980). Joint motion in the absence of normal loading does not maintain normal articular cartilage. Arthritis Rheum 23:325-334.[Medline] [Order article via Infotrieve]
• Radin EL, Ehrlich MG, Chernack R., Abernethy P., Paul IL, Rose RM ( 1978). Effect of repetitive impulsive loading on the knee joints of rabbit. Clin Orthop Rel Res 131:288-293.[Medline] [Order article via Infotrieve]
• Rodan GA, Bourret LA, Cutler LS (1977). Membrane changes during cartilage maturation. J Cell Biol 72:493-501.[Abstract/Free Full Text]
• Rodan GA, Mensi T., Harvey A. (1975). A quantitative method for the application of compressive forces to bone in tissue culture. Calcif Tissue Res 18:125-131.[CrossRef][Medline] [Order article via Infotrieve]
• Tilton FE, Degioanni Jjc, Schneider VS (1980). Long-term follow-up of skylab bone demineralization. Aviat Space Environ Med 51:1209-1213.[Medline] [Order article via Infotrieve]
• Yeh C-K., Rodan GA (1984). Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons. Calcif Tissue Int 36(Suppl):67S-71S.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Dental Research, Vol. 72, No. 9, 1351-1355 (1993)
DOI: 10.1177/00220345930720091601


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Inoue, H. Articles by Sakuda, M. Search for Related Content PubMed PubMed Citation Articles by Inoue, H. Articles by Sakuda, M. Social Bookmarking                         What's this?