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Immunohistochemical Studies on the Distributions and Age-related Changes of Types I and III Collagen in the Oral Mucosa of Mice

Second Department of Anatomy, Department of Preventive Dentistry, Faculty of Dentistry, Kyushu University, Fukuoka, Japan

Y. Ohsaki

Second Department of Anatomy

K. Nagata

Second Department of Anatomy

K. Kurisu

First Department of Anatomy, Osaka University Faculty of Dentistry, Suita, Osaka, Japan

The distribution of types I and III collagen in mouse oral mucosa and the age-related changes over 3 days to 2 years of age were examined by immuno-fluorescence and -electron microscopy, with use of affinity-purified polyclonal antibodies. Immunofluorescence microscopy revealed that types I and III collagen existed in all tissues and at all ages examined. The staining intensity for type I collagen was stronger and increased more markedly in the lamina propria of the hard palate (HPlp) and gingiva (G), compared with that in the submucosa of the hard palate (HPsm) and the buccal mucosa (BM). The staining intensity for type III collagen was strong and increased markedly with age in all connective tissues examined. Examination of immunogold-labeled tissues demonstrated that most of the collagen fibrils were labeled for both type I and type III collagen, which suggests that they were hybrid fibrils containing both types of collagen. The quantitative evaluation of the labeling densities of gold particles revealed that the labeling density of type III collagen in BM and HPsm was higher and increased more rapidly during growth than in HPIp and G, while the labeling density of type I collagen was higher in HPIp and G. The fibril diameters in HPIp were larger and increased more rapidly during growth than in BM and HPsm. These studies are the first to demonstrate the distribution of types I and III collagen and their age-related changes in mouse oral mucosa. The results support previous suggestions that type I collagen may contribute to the stability of the connective tissue organization and that type III collagen may play an important role in the flexibility of the connective tissue of oral mucosa as well as in the regulation of the collagen fibril diameters in the tissue.

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Journal of Dental Research, Vol. 72, No. 9, 1336-1343 (1993)
DOI: 10.1177/00220345930720091401


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This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Xu, L.X. Articles by Kurisu, K. Search for Related Content PubMed PubMed Citation Articles by Xu, L.X. Articles by Kurisu, K. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB GOLD Social Bookmarking                         What's this?