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Protein Analysis of Human von Ebner Saliva and a Method for its Collection from the Foliate Papillae

Division of Basic Sciences, New York University College of Dentistry, 345 E. 24th Street, New York, New York 10010

S. D'Abundo

Division of Basic Sciences, New York University College of Dentistry, 345 E. 24th Street, New York, New York 10010

R.B. Field

Georgetown University Medical Center, Department of Pediatrics, and Department of Veterans Affairs Medical Center, Oral Pathology Research Lab., Washington, DC

H. Schmale

University of Hamburg, Department of Cell Biology and Clinical Neurobiology, Hamburg, Germany

The lingual serous glands of von Ebner are located close to the foliate and circumvallate papillae. Saliva secreted by these glands provides the immediate environment of the taste buds, and it has been hypothesized that it modulates taste perception. The purpose of this study was to develop a technique for collection of unstimulated and stimulated saliva from human von Ebner glands. Saliva was collected under resting conditions and after application of various gustatory stimuli (sweet, sour, salt, and bitter) by insertion of periostrips into the folds of the foliate papillae of healthy human volunteers. Stimulated saliva was also collected in glass microcapillaries or micropipettes. The flow-rates of unstimulated von Ebner saliva were 2.3 ± 0.6 (S.E.) µL/min and 4.5 ± 1.2 (S.E.) µL/min with 1% citric acid stimulation. The protein content was 2.5 ± 0.5 (S.E.) mg/mL. The SDS gel electrophoretic profile of von Ebner saliva revealed two protein bands of Mr 18,000 that were identified on Western blots as von Ebner gland (VEG) proteins. Although lingual lipase activity was detected at very low levels by enzyme assay, this protein was not detected on Western blots. This collection technique should prove useful for analysis of specific functions associated with secretion from von Ebner glands.

REFERENCES

• Armand M., Borel P., Cara L., Senft M., Chautan M., Lafont H., Lairon D. ( 1990). Adaptation of lingual lipase to dietary fat in rats. J Nutr 120:1148-1156.[Abstract/Free Full Text]
• Beidler J. (1990). Protein composition of the von Ebner gland secretions (abstract). Chem Senses 15:552.
• Blaker M., Kock K., Ahlers C., Buck F., Schmale H. (1993). Molecular cloning of human von Ebner's gland protein, a member of the lipocalin superfamily highly expressed in lingual salivary glands. Biochim Biophys Acta (in press).
• DeNigris SJ, Hamosh M., Kasbekar DK, Lee TC, Hamosh P. (1988). Lingual and gastric lipase: species differences in the origin of prepancreatic digestive lipases and in the localization of gastric lipase. Biochim Biophys Acta 959:38-45.[Medline] [Order article via Infotrieve]
• Field RB, Scow RO ( 1983). Purification and characterization of rat lingual lipase. J Biol Chem 258:14563-14569.[Abstract/Free Full Text]
• Field RB, Hand AR ( 1987). Secretion of lingual lipase and amylase from rat lingual serous glands. Am J Physiol 253:G217-G225.[Medline] [Order article via Infotrieve]
• Field RB, Spielman AI, Hand AR ( 1989). Purification of lingual amylase from serous glands of rat tongue and characterization of rat lingual amylase and lingual lipase. J Dent Res 68:139-145.[Abstract/Free Full Text]
• Gurkan S., Bradley RM (1987). Autonomic control of von Ebner's lingual salivary glands and implications for taste sensation. Brain Res 419:287-293.[CrossRef][Medline] [Order article via Infotrieve]
• Gurkan S., Bradley RM (19881. Secretions of von Ebner's glands influence responses from taste buds in rat circumvallate papillae. Chem Senses 13:655-661.
• Hamosh M., Klaeveman HL, Wolf RO, Scow RO ( 1975). Pharyngeal lipase and digestion of dietary triglyceride in man. J Clin Invest 55:908-913.[CrossRef][Medline] [Order article via Infotrieve]
• Hamosh M., Burns WA (1977). Lipolytic activity of human lingual glands (Ebner). Lab Invest 37:603-608.[Medline] [Order article via Infotrieve]
• Kock K., Blaker M., Schmale H. (1992). Postnatal development of von Ebner's glands: accumulation of a protein of the lipocalin superfamily in taste papillae of rat tongue. Cell Tissue Res 267:313-320.[CrossRef][Medline] [Order article via Infotrieve]
• Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.[CrossRef][Medline] [Order article via Infotrieve]
• Moreau H., Laugier R., Gargouri Y., Ferrato F. Verger R. (1988a). Human preduodenal lipase is entirely of gastric fundic origin. Gastroenterology 95:1221-226.[Medline] [Order article via Infotrieve]
• Moreau H., Gargouri Y., Lecat D., Junien JJ, Verger R. (1988b). Screening of preduodenal lipases in several mammals. Biochim Biophys Acta 958:247-252.[Medline] [Order article via Infotrieve]
• Schmale H., Holtgreve-Grez H., Christiansen H. (1990). Possible role for salivary gland protein in taste reception indicated by homology to lipophilic-ligand carrier proteins. Nature 343:366-369.[CrossRef][Medline] [Order article via Infotrieve]
• Schmale H., Ahlers C., Blaker M., Kock K., Spielman AI (1993). Perireceptor events in taste. In: The molecular basis of smell and taste transduction. London (UK): The Ciba Foundation (in press).
• Spielman AI (1990). Interaction of saliva and taste. J Dent Res 69:838-843.[Abstract/Free Full Text]
• Towbin H., Staehelin T., Gordon J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Procedure and some applications. Proc Natl Acad Sci USA 76:4350-4354.[Abstract/Free Full Text]

Journal of Dental Research, Vol. 72, No. 9, 1331-1335 (1993)
DOI: 10.1177/00220345930720091301


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